1887

Abstract

Abstract

The name subsp. was recently proposed for strains belonging to the genus that were isolated from the gastric mucosa of ferrets because of the high levels of deoxyribonucleic acid relatedness (85 to 100%) of these strains to the type strain of Subsequent deoxyribonucleic acid relatedness studies done independently in three laboratories by three different methods indicate that the original results were in error. Strains isolated from ferrets in the United States, England, and Australia are members of a single species that is substantially different from (10 to 20% relatedness at 50°C and 3 to 10% relatedness at 65°C as determined by the hydroxyapatite method; less than 10% relatedness at 65°C as determined by the nylon membrane blot method; and 30 to 49% relatedness at 66 to 68°C as determined by the spectrophotometric method). The strains isolated from ferrets also differ from human C. strains in their ability to reduce nitrates, their negative leucine arylamidase reaction, their susceptibility to nalidixic acid, and their resistance to cephalothin. The strains from ferrets possess both polar and lateral flagella, whereas only polar flagella have been demonstrated in human strains. Thus, the strains isolated from ferrets are members of a species that is distinct from , and we propose elevating subsp. to species status as sp. nov.

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/content/journal/ijsem/10.1099/00207713-39-3-301
1989-07-01
2024-03-28
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References

  1. Blackall L. L., Hayward A. C., Sly L. I. 1985; Cellulolytic and dextranolytic gram-negative bacteria: revival of the genus Cellovibrio. J. Appl. Bacteriol. 59:81–97
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  3. Fox J. G., Edrise B. M., Cabot E. B., Beaucage C., Murphy J. C., Prostak K. S. 1986; Campylobacter-like organisms isolated from gastric mucosa in ferrets. Am. J. Vet. Res. 47:236–239
    [Google Scholar]
  4. Fox J. G., Taylor N. S., Edmonds P., Brenner D. J. 1988; Campylobacter pylori subsp. mustelae subsp. nov. isolated from the gastric mucosa of ferrets (Mustelae putorius furo), and an emended description of Campylobacter pylori. Int. J. Syst. Bacteriol. 38:367–370
    [Google Scholar]
  5. Gillis M. M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur. J. Biochem. 12:143–153
    [Google Scholar]
  6. Goodwin C. S., Blake P., Blincow E. 1986; The minimum inhibitory and bactericidal concentrations of antibiotics and anti-uker agents against Campylobacter pyloridis. J. Antimicrob. Chemother. 17:309–314
    [Google Scholar]
  7. Huss V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridisation from renaturation rates. Syst. Appl. Microbiol. 4:184–192
    [Google Scholar]
  8. Lambert T., Megraud F., Gerbaud G., Courvalin P. 1986; Susceptibility of Campylobacter pyloridis to 20 antimicrobial agents. Antimicrob. Agents Chemother. 30:510–511
    [Google Scholar]
  9. Majewski S. I. H., Goodwin C. S. 1988; Restriction endonuclease analysis of the genome of Campylobacter pylori with a rapid extraction method: evidence for considerable genomic variation. J. Infect. Dis. 157:465–471
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  11. Marshall B. J., Goodwin C. S. 1987; Revised nomenclature of Campylobacter pyloridis. Int. J. Syst. Bacteriol 37:68
    [Google Scholar]
  12. Marshall B. J., McGechie D. B., Rogers P. A., Glancy R. J. 1985; Pyloric Campylobacter infection and gastroduodenal disease. Med. J. Aust. 142:439–444
    [Google Scholar]
  13. Marshall B. J., Royce H., Annear D. I., Goodwin C. S., Pearman J. W., Warren J. R., Armstrong J. A. 1984; Original isolation of Campylobacter pyloridis from human gastric mucosa. Microbios Lett. 25:83–88
    [Google Scholar]
  14. Rathbone B. J., West A. P., Wyatt J. I., Johnson A. W., Tompkins D. S., Heatley R. V. 1986; Campylobacter pyloridis, urease, and gastric ulcers. Lancet i:400–401
    [Google Scholar]
  15. Sly L. I., Blackall L. L., Kraat P. C., Tao T.-S., Sangkhobol V. 1986; The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J. Microbiol. Methods 5:139–156
    [Google Scholar]
  16. Tompkins D. S., Wyatt J. I., Rathbone B. J., West A. P. 1988; The characterization and pathological significance of gastric campylobacter-like organisms in the ferret: a model for chronic gastritis?. Epidemiol. Infect. 101:269–278
    [Google Scholar]
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