RT Journal Article SR Electronic(1) A1 ZHAO, HONGXUE A1 YANG, DECHENG A1 WOESE, CARL R. A1 BRYANT, MARVIN P.YR 1990 T1 Assignment of Clostridium bryantii to Syntrophospora bryantii gen. nov., comb. nov. on the Basis of a 16S rRNA Sequence Analysis of Its Crotonate-Grown Pure Culture JF International Journal of Systematic and Evolutionary Microbiology, VO 40 IS 1 SP 40 OP 44 DO https://doi.org/10.1099/00207713-40-1-40 PB Microbiology Society, SN 1466-5034, AB The Clostridium bryantii-Methanospirillum hungatei syntrophic coculture, grown on caproate, was adapted to grow on crotonate. Then, C. bryantii was isolated in pure culture from crotonate bottle plates. A 16S rRNA sequence analysis of the pure subculture revealed that, as a member of the gram-positive phylum, it was not closely related to any of the Clostridium species with which it was compared or to any of the other clusters in the gram-positive phylum with which it was compared. However, it was closely related to another syntrophic fatty acid-degrading bacterium, Syntrophomonas wolfei. On the basis of its phylogeny, physiology, and cell wall ultrastructure, we propose assignment of C. bryantii to Syntrophospora bryantii gen. nov., nov. comb., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-40-1-40