1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 40, Issue 2

sp. nov., a Methylotrophic Methanogen from an Alkaline, Saline Aquifer Free

Abstract

WAL1 (= OGI 99 = DSM 5435) (T = type strain) was isolated from an anoxic aquifer (pH 10, with 100 g of dissolved solids per liter of pore water) 3 m deep near Alkali Lake, an alkaline, desert lake in south central Oregon. An examination of the subsurface sediments revealed no methanogens that were capable of growth on methanogenic substrates, such as H-CO, formate, or acetate, which is consistent with the results of other studies of hypersaline, sulfate-containing anoxic environments. Strain WAL1 grew on trimethylamine and grew slowly on methanol or dimethylsulfide, but did not catabolize H-CO, formate, or acetate. The cells were irregular coccoids (diameter, 1 to 1.5 μm), and cells growing in liquid media also formed clumps of 2 to 15 or more cells. The cells were mesophilic and required one or more vitamins present in yeast extract. Like the only previously described strain of alkaliphilic, methylotrophic methanogen ( WeN5), strain WAL1 grew most rapidly in medium of moderate salinity; strain WAL1 grew well in the presence of 0.1 to 1.4 M Na and grew most rapidly at an Na concentration of 0.35 M (specific growth rate, 0.1 h). Best growth occurred with about 50 mM Mg and at a pH of 8.4 to 9.0. K appeared to be required, with 13 to 130 mM K supporting most rapid growth. The guanine-plus-cytosine content of the DNA was 40.9 ± 0.1 mol%.

  • Published Online:
Copyright © 1990 International Union of Microbiological Societies
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-40-2-111
1990-04-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/40/2/ijsem-40-2-111.html?itemId=/content/journal/ijsem/10.1099/00207713-40-2-111&mimeType=html&fmt=ahah

References

  1. Abram J. W., Nedwell D. B. 1978; Inhibition of methanogenesis by sulfate-reducing bacteria competing for transferred hydrogen. Arch. Microbiol. 117:89–92
     [Google Scholar]
  2. Aldrich H. C., Beimborn D. B., Schönheit P. 1987; Creation of artifactual internal membranes during fixation of Methanobacterium thermoautotrophicum. Can. J. Microbiol. 33:844–849
     [Google Scholar]
  3. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: réévaluation of a unique biological group. Microbiol. Rev. 43:260–296
     [Google Scholar]
  4. Boone D. R., Mah R. A. 1987; Effects of calcium, magnesium, pH, and extent of growth on the morphology of Methanosarcina mazei S-6. Appl. Environ. Microbiol. 54:16991700
     [Google Scholar]
  5. Boone D. R., Whitman W. B. Minimal standards for the description of methanogenic bacteria. Int. J. Syst. Bacteriol. 38:212–219
     [Google Scholar]
  6. Boone D. R., Worakit S., Mathrani I. M., Mah R. A. 1986; Alkaliphilic methanogens from high-pH lake sediments. J. Syst. Appl. Microbiol. 7:230–234
     [Google Scholar]
  7. Doetsch R. N. 1981 Determinative methods of light microscopy. 21–23 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
     [Google Scholar]
  8. Hungate R. E. 1969 A roll tube method for cultivation of strict anaerobes. 117–132 Norris R., Ribbons D. W.ed Methods in microbiology 3B Academic Press, Inc.; New York:
     [Google Scholar]
  9. Kiene R. P., Oremland R. S., Catena A., Miller L. G., Capone D. G. 1986; Metabolism of reduced methylated sulfur compounds in anaerobic sediments and by a pure culture of an estuarine methanogen. Appl. Environ. Microbiol. 52:1037–1045
     [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
     [Google Scholar]
  11. Mathrani I. M., Boone D. R., Mah R. A., Fox G. E., Lau P. P. 1988; Methanohalobium zhilinae, gen. nov. sp. nov., an alkaliphilic, halophilic, methylotrophic methanogen. Int. J. Syst. Bacteriol. 38:139–142
     [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the guanine-plus-cytosine content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39:159–167
     [Google Scholar]
  13. Oremland R. S., Polcin S. 1982; Methanogenesis and sulfate reduction: competitive and noncompetitive substrates in estuarine sediments. Appl. Environ. Microbiol. 44:1270–1276
     [Google Scholar]
  14. Pankow J. F., Johnson R. L., Houck J. E., Brillante S. M., Bryan W. J. 1984; Migration of chlorophenolic compounds at the chemical waste disposal site at Alkali Lake, Oregon. I. Site description and ground-water flow. Ground Water 22:593–601
     [Google Scholar]
  15. Paterek J. R., Smith P. H. 1985; Isolation and characterization of a halophilic methanogen from Great Salt Lake. Appl. Environ. Microbiol. 50:877–881
     [Google Scholar]
  16. Paterek J. R., Smith P. H. 1988; Methanohalophilus mahii gen. nov., sp. nov., a methylotrophic halophilic methanogen. Int. J. Syst. Bacteriol. 38:122–123
     [Google Scholar]
  17. Powell G. E. 1983; Interpreting gas kinetics of batch cultures. Biotechnol. Lett. 5:437–440
     [Google Scholar]
  18. Sowers K. R., Ferry J. G. 1983; Isolation and characterization of a methylotrophic marine methanogen, Methanococcoides methylutens gen. nov., sp. nov. Appl. Environ. Microbiol. 45:684–690
     [Google Scholar]
  19. Winfrey M. R., Zeikus J. G. 1977; Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments. Appl. Environ. Microbiol. 33:275–281
     [Google Scholar]
  20. Yu I. K., Kawamura F. 1987; Halomethanococcus doii gen. nov., sp. nov.: an obligately halophilic methanogenic bacterium from solar salt ponds. J. Gen. Appl. Microbiol. 33:303–310
     [Google Scholar]
  21. Zhilina T. N., Zavarzin G. A. 1973; Trophic relationships between Methanosarcina and its associates. Mikrobiologiya 42:266–273
     [Google Scholar]
  22. Zhilina T. N., Zavarzin G. A. 1987; Methanohalobium evistigatus, n. gen., n. sp., the extremely halophilic methanogenic archaebacterium. Dokl. Akad. Nauk SSSR 293:464–468
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-40-2-111
Loading
Methanohalophilus oregonense sp. nov., a Methylotrophic Methanogen from an Alkaline, Saline Aquifer
Int J Syst Evol Microbiol 40, 111 (1990); https://doi.org/10.1099/00207713-40-2-111
/content/journal/ijsem/10.1099/00207713-40-2-111
/content/journal/ijsem/10.1099/00207713-40-2-111
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error