1887

Abstract

Mycoplasmas which were distinct from were isolated from the conjunctivas of Chinese hamsters. Two clones, which were derived from a single colony and were obtained on separate occasions, were examined in detail for morphology, growth, and biochemical characteristics. These clones were indistinguishable from each other and had the following properties: Guanine-plus-cytosine content of 29 mol%, requirement for sterol, facultatively anaerobic, and positive for glucose metabolism. They were serologically distinct from and all 94 other previously described and spp. One of them, strain 128 (= NCTC 11712), is designated the type strain of a new species, .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-41-1-21
1991-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/41/1/ijsem-41-1-21.html?itemId=/content/journal/ijsem/10.1099/00207713-41-1-21&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Whittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
    [Google Scholar]
  2. Barber T. L., Fabricant J. 1971; Identification of Mycoplasmatales: characterization procedures. Appl. Microbiol 21:600–605
    [Google Scholar]
  3. Boatman E. S. 1979 Morphology and ultrastructure of the Mycoplasmatales,. 63–102 Barile M. F., Razin S.ed The mycoplasmas 1 Academic Press, Inc.; New York:
    [Google Scholar]
  4. Clyde W. A. Jr. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol. 92:958–965
    [Google Scholar]
  5. Edward D. G. ff 1971; Determination of sterol requirement for Mycoplasmatales. J. Gen. Microbiol. 69:205–210
    [Google Scholar]
  6. Edward D. G. ff, Fitzgerald W. A. 1951; Cholesterol in the growth of organisms of the pleuropneumonia group. J. Gen. Microbiol. 5:576–586
    [Google Scholar]
  7. Fabricant J., Freundt E. A. 1967; Importance of extension and standardization of laboratory tests for the identification and classification of mycoplasma. Ann. N. Y. Acad. Sci. 143:50–58
    [Google Scholar]
  8. Fallon R. J., Whittlestone P. 1969 Isolation, cultivation and maintenance of mycoplasmas. 211–267 Norris J. R., Robbins D. W.ed Methods in microbiology 3B Academic Press, Inc.; New York:
    [Google Scholar]
  9. Freundt E. A., Andrews B. E., Erno H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium-polyanethol sulfonate and digitonin. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Grig. Reihe A 225:104–112
    [Google Scholar]
  10. Gross-Bellard M., Oudet P., Chambon P. 1973; Isolation of high molecular weight DNA from mammalian cells. Eur. J. Biochem. 36:32–38
    [Google Scholar]
  11. Hill A. C. 1971; Mycoplasma caviae, a new species. J. Gen. Microbiol. 65:109–113
    [Google Scholar]
  12. Hill A. C. 1974 Mycoplasmas of small animal hosts. 311–316 Bové J. M., Duplan J. F.ed Les mycoplasmes mycoplasmas Institut National de la Sante et de la Recherche Medicale; Paris:
    [Google Scholar]
  13. Hill A. C. 1977; The metabolic inhibition test for mycoplasmas based on phosphatase production. J. Hyg. 79:391–393
    [Google Scholar]
  14. Hill A. C. 1983; Mycoplasma cricetuli, a new species from the conjunctiva of Chinese hamsters. Int. J. Syst. Bacteriol. 33:113–117
    [Google Scholar]
  15. Klieneberger-Nobel E. 1962 Morphology of pleuropneumonialike organisms. 23–56 Klieneberger-Nobel E.ed Pleuropneumonia-like organisms (PPLO). Mycoplasmataceae Academic Press, Inc.; New York:
    [Google Scholar]
  16. Leach R. H. 1976; The inhibitory effect of arginine on growth of some mycoplasmas. J. Appl. Bacteriol. 41:259–264
    [Google Scholar]
  17. Manchee R. J., Taylor-Robinson D. 1968; Haemadsorption and haemagglutination by mycoplasmas. J. Gen. Microbiol. 50:465–478
    [Google Scholar]
  18. Morton H. E., Roberts R. J. 1967; Production of antimycoplasma (PPLO) antibodies in rabbits. Proc. Soc. Exp. Biol. Med. 125:538–543
    [Google Scholar]
  19. Mouches C., Bové J. M. 1983 Electrophoretic characterization of mycoplasma proteins. 241–255 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press, Inc.; New York:
    [Google Scholar]
  20. PoIak-Vogelzang A. Personal communication
  21. Polak-Vogelzang A. A., Hagenaars R., Nagel S. 1978; Evaluation of an indirect immunoperoxidase test for identification of Acholeplasma and Mycoplasma. J. Gen. Microbiol. 106:241–249
    [Google Scholar]
  22. Purcell R. H., Taylor-Robinson D., Wong D. C., Chanock R. M. 1966; A color test for the measurement of antibody to the non-acid-forming human mycoplasma species. Am. J. Epidemiol. 84:51–66
    [Google Scholar]
  23. Purcell R. H., Taylor-Robinson D., Wong D., Chanock R. M. 1966; Color test for the measurement of antibody to T-strain mycoplasmas. J. Bacteriol. 92:6–12
    [Google Scholar]
  24. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol. 102:306–310
    [Google Scholar]
  25. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol. 4:430–443
    [Google Scholar]
  26. Shepard M. C., Howard D. R. 1970; Identification of “T” mycoplasmas in primary agar cultures by means of a direct test for urease. Ann. N.Y. Acad. Sci. 174:809–819
    [Google Scholar]
  27. Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes. Int. J. Syst. Bacteriol. 29:172–180
    [Google Scholar]
  28. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; A color test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg. 64:91–104
    [Google Scholar]
  29. Taylor-Robinson D., Williams M. H., Haig D. A. 1968; The isolation and comparative biological and physical characteristics of T-mycoplasmas of cattle. J. Gen. Microbiol. 54:33–46
    [Google Scholar]
  30. Timms L. 1967; Isolation and identification of avian mycoplasmas. J. Med. Lab. Technol. 24:79–89
    [Google Scholar]
  31. Tully J. G. 1965; Biochemical, morphological and serological characterization of mycoplasma of murine origin. J. Infect. Dis. 115:171–185
    [Google Scholar]
  32. Tully J. G., Razin S. 1969; Characteristics of a new sterol-nonrequiring mycoplasma. J. Bacteriol. 98:970–978
    [Google Scholar]
  33. Venable J. H., Coggeshall R. 1965; A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25:407
    [Google Scholar]
  34. Williams C. O., Wittier R. G. 1971; Hydrolysis of esculin and phosphatase production by members of the order Mycoplasmatales which do not require sterol. Int. J. Syst. Bacteriol. 21:73–77
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-41-1-21
Loading
/content/journal/ijsem/10.1099/00207713-41-1-21
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error