1887

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Volume 41, Issue 1

sp. nov. and sp. nov., Two New Species from Harbor Seals ( L.) Free

Abstract

A total of 120 mycoplasma strains were recovered from 97 of 265 diseased seals investigated during the seal epidemic in the North Sea and in the Baltic Sea in 1988. Mycoplasmas were isolated from the respiratory tracts (including lungs), hearts, brains, and eyes of the seals. Thirty strains were filter cloned and investigated for their morphological, biochemical, and serological characteristics compared with the characteristics of previously described species. The results of an indirect immunofluorescence test, a growth inhibition test, and an immunobinding assay showed that these strains belong to two new species, for which the names and are proposed. (17 strains) did not ferment glucose or hydrolyze arginine but did reduce tetrazolium chloride and potassium tellurite and produced films and spots. (13 strains) metabolized arginine but not glucose and produced phosphatase but did not reduce tetrazolium chloride and potassium tellurite. Both species lysed sheep erythrocytes but did not absorb sheep or guinea pig erythrocytes. The type strain of is strain 852 (= ATCC 49639), and the type strain of is strain 1049 (= ATCC 49640).

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Copyright © 1991 International Union of Microbiological Societies
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1991-01-01
2024-04-25
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References

  1. Albers A. C., Fletcher R. D. 1982; Simple method for quantification of viable mycoplasmas. Appl. Environ. Microbiol. 43:958–960
     [Google Scholar]
  2. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
     [Google Scholar]
  3. Clyde W. A. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol. 92:958–965
     [Google Scholar]
  4. Del Giudice R. A., Robillard F., Carski T. R. 1967; Immunofluorescence identification of mycoplasma on agar by use of incident illumination. J. Bacteriol. 93:1205–1209
     [Google Scholar]
  5. Flossdorf J. 1983; A rapid method for the determination of the base composition of bacterial DNA. J. Microbiol. Methods 1:305–311
     [Google Scholar]
  6. Freundt E. A., Andrews B. E., Erno H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium-poly-anetholsulfonate and digitonin. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1. Orig. Reihe A 225:104–112
     [Google Scholar]
  7. Geraci J. R., St. Aubin D. J., Barker I. K., Hinshaw V. S., Webster R. G., Ruhnke H. L. 1984; Susceptibility of grey (Halichoerus grypus) and harp (Phoca groenlandica) seals to the influenza virus and mycoplasma of epizootic pneumonia of harbour seals (Phoca vitulina). Can. J. Fish. Aquat. Sci. 41:151–156
     [Google Scholar]
  8. Geraci J. R., St Aubin D. J., Barker I. K., Webster R. G., Hinshaw V. S., Bean W. J., Ruhnke H. L., Prescott J. H., Early G., Baker A. S., Madoff S., Schooley R. T. 1982; Mass mortality of harbour seals: pneumonia associated with influenza A virus. Science (Washington, D.C.) 215:1129–1131
     [Google Scholar]
  9. Kennedy S., Smyth J. A., Cush P. F., McCullough S. J., Allan G. M., McQuaid S. 1988; Viral distemper now found in porpoises. Nature (London) 336:21
     [Google Scholar]
  10. Kennedy S., Smyth J. A., McCullough S. J., Allan G. M., McNeilly F. 1988; Confirmation of cause of recent seal deaths. Nature (London) 335:804
     [Google Scholar]
  11. Kirchhoff H., Beyene P., Fischer M., Flossdorf J., Heitmann J., Khattab B., Lopatta D., Rosengarten R., Seidel G., Yousef C. 1987; Mycoplasma mobile sp. nov., a new species from fish. Int. J. Syst. Bacteriol. 37:192–197
     [Google Scholar]
  12. Kirchhoff H., Binder A., Liess B., Friedhoff K. T., Pohlenz J., Stede M., Willhaus T. 1989; Isolation of mycoplasmas from diseased seals. Vet. Rec. 124:513–514
     [Google Scholar]
  13. Kirchhoff H., Rosengarten R. 1984; Isolation of a motile mycoplasma from fish. J. Gen. Microbiol. 130:2439–2445
     [Google Scholar]
  14. Kotani H., McGarrity G. J. 1986; Identification of mycoplasma colonies by immunobinding. J. Clin. Microbiol. 23:783–785
     [Google Scholar]
  15. Madoff S., Schooley R. T., Ruhnke H. C., Del Giudice R. A., Barker J. K., Geraci J., Baker A. S. 1982; Mycoplasmal pneumonia in phocid (harbour) seals. Rev. Infect. Dis. 4:1982
     [Google Scholar]
  16. Mahy B. W. J., Barrett T., Evans S., Anderson E. C., Bostock C. J. 1988; Characterization of a seal morbillivirus. Nature (London) 336:115
     [Google Scholar]
  17. McCourty C. 1988; Seal epidemic still spreading. Nature (London) 334:553
     [Google Scholar]
  18. Morton H. E., Roberts R. J. 1966; Production of antimycoplasma (PPLO) antibodies in rabbits. Proc. Soc. Exp. Biol. Med. 125:538–542
     [Google Scholar]
  19. Osterhaus A. D. M. E. 1988; Seal death. Nature (London) 334:301–302
     [Google Scholar]
  20. Osterhaus A. D. M. E., Groen J., De P., Vries F., Uytde Haag G. C. M., Klingeborn B., Zarnke R. 1988; Canine distemper virus in seals. Nature (London) 335:403–404
     [Google Scholar]
  21. Osterhaus A. D. M. E., Vedder E. J. 1988; Identification of a virus causing seal death. Nature (London) 335:20
     [Google Scholar]
  22. Sobeslavsky O., Prescott B., Chanock R. M. 1968; Absorption of Mycoplasma pneumoniae to neuraminic acid receptors of various cells and possible role in virulence. J. Bacteriol. 96:695–705
     [Google Scholar]
  23. Stadtländer C., Hartmann D., Binder A., Kirchhoff H. 1989; Investigations of seal mycoplasmas for their cytotoxic potential on tracheal organ cultures of SPF and gnotobiotic rats. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Grig. 272:216–224
     [Google Scholar]
  24. Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for description of new species of the class Mollicutes. Int. J. Syst. Bacteriol. 29:172–180
     [Google Scholar]
  25. Subcommittee on the Taxonomy of Mollicutes 1988; Minutes of the interim meeting, 25 and 28 August 1986, Birmingham, Alabama. Int. J. Syst. Bacteriol. 38:226–230
     [Google Scholar]
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Mycoplasma phocarhinis sp. nov. and Mycoplasma phocacerebrale sp. nov., Two New Species from Harbor Seals (Phoca vitulina L.)
Int J Syst Evol Microbiol 41, 39 (1991); https://doi.org/10.1099/00207713-41-1-39
/content/journal/ijsem/10.1099/00207713-41-1-39
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