Differentiation of Xanthomonas campestris pv. Citri Strains by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis of Proteins, Fatty Acid Analysis, and DNA-DNA Hybridization Vauterin, L. and Yang, P. and Hoste, B. and Vancanneyt, M. and Civerolo, E. L. and Swings, J. and Kersters, K.,, 41, 535-542 (1991), doi = https://doi.org/10.1099/00207713-41-4-535, publicationName = Microbiology Society, issn = 1466-5026, abstract= A total of 61 strains, including members of all five currently described pathogenicity groups of Xanthomonas campestris pv. citri (groups A, B, C, D, and E) and representing a broad geographical diversity, were compared by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins, gas chromatographic analysis of fatty acid methyl esters, and DNA-DNA hybridization. We found that all of the pathogenicity groups were related to each other at levels of DNA binding of more than 60%, indicating that they all belong to one species. Our results do not confirm a previous reclassification of X. campestris pathogens isolated from citrus in two separate species (Gabriel et al., Int. J. Syst. Bacteriol. 39:14-22, 1989). Pathogenicity groups A and E could be clearly delineated by the three methods used, and group A was the most homogeneous group. The delineation of pathogenicity groups B, C, and D was not clear on the basis of the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins and gas chromatography of fatty acid methyl esters, although these groups constituted a third subgroup on the basis of the DNA homology results., language=, type=