1887

Abstract

Helical organisms with novel ultrastructural characteristics were isolated from the intestinal mucosa of rats and mice. These bacteria were characterized by the presence of 9 to 11 periplasmic fibers which appeared as concentric helical ridges on the surface of each cell. The cells were motile with a rapid corkscrewlike motion and had bipolar tufts of 10 to 14 sheathed flagella. The bacteria were microaerophilic, nutritionally fastidious, and physiologically similar to species and but could be differentiated from these organisms by their unique cellular ultrastructure. Using 16S rRNA sequencing, we found that strain ST1 (T = type strain) was related to previously described species, “,” and . The closest relatives of strain ST1 were and “” (average similarity value, 96%). On the basis of phylogenetic data, strain ST1 (= ATCC 49282) represents a new species of the genus , for which we propose the name .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-42-1-27
1992-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/42/1/ijs-42-1-27.html?itemId=/content/journal/ijsem/10.1099/00207713-42-1-27&mimeType=html&fmt=ahah

References

  1. Archer J. R., Romero S., Ritchie A. E., Hamacher M. E., Stenier B. M., Bryner J. H., Schell R. F. 1988; Characterization of an unclassified microaerophilic bacterium associated with gastroenteritis. J. Clin. Microbiol. 26:101–105
    [Google Scholar]
  2. Blackall L. L., Hayward A. C., Sly L. I. 1985; Cellulolytic and dextranolytic gram-negative bacteria: revival of the genus Cellvibrio. J. Appl. Bacteriol. 59:81–97
    [Google Scholar]
  3. Brock F. M., Murray R. G. E. 1988; The ultrastructure and ATPase nature of polar membrane in Campylobacter jejeuni. Can. J. Microbiol. 34:594–604
    [Google Scholar]
  4. Bryner J. H. 1988 Flexispira rappini, gen. nov., sp. nov. A motile, urease-producing rod similar to Campylobacter pyloridis. 440 Kaijser B., Falsen E.ed Campylobacter IV Gotema, Kungalv; Sweden:
    [Google Scholar]
  5. Bryner J. H., Ritchie A. E., Pollet L., Kirkbride A., Collins J. E. 1987; Experimental infection and abortion of pregnant guinea pigs with a unique spirillum-like bacterium isolated from aborted ovine fetuses. Am. J. Vet. Res. 48:91–95
    [Google Scholar]
  6. Buck G. E., Gourley W. K., Lee, Subramanyam W. K., Latimer J. M., DiNuzzo A. R. 1986; Relation of Campylobacter pyloridis to gastritis and peptic ulcer. J. Infect. Dis. 15:664–669
    [Google Scholar]
  7. Coulton J. W., Murray R. G. E. 1978; Cell envelope association of Aquaspirillum serpens flagella. J. Bacteriol. 135:1037
    [Google Scholar]
  8. Davis C. P., Mulcahy D., Takeuchi A., Savage D. C. 1972; Location and description of spiral-shaped microorganisms in the normal rat cecum. Infect. Immun. 6:184–192
    [Google Scholar]
  9. Dent J. C., McNulty C. A. M., Uff J. C., Wilkinson S. P., Gear M. W. L. 1987; Spiral organisms in the gastric antrum. Lancet i:96
    [Google Scholar]
  10. Doenges J. L. 1939; Spirochetes in the gastric glands of macacus rhesus and of man without related disease. Arch. Pathol. 25:469–477
    [Google Scholar]
  11. Erlandsen S. L., Chase D. G. 1972; Paneth cell function: phagocytosis and intracellular digestion of intestinal microorganisms. II. Spiral microorganism. J. Ultrastruct. Res. 41:319–333
    [Google Scholar]
  12. Ferrero R. L., Lee A. 1991; The importance of urease in acid protection for the gastric-colonising bacteria, Helicobacter pylori and Helicobacter felis sp. nov. Microbiol. Ecol. Health Dis. 4:121–134
    [Google Scholar]
  13. Ferris F. G., Beveridge T. J., Marceau-Day M. L., Larson A. D. 1984; Structure and cell envelope associations of flagella basal complexes of Vibrio cholerae and Campylobacter fetus. Can. J. Microbiol. 30:322–333
    [Google Scholar]
  14. Fox J. G., Chilvers T., Goodwin C. S., Taylor N. S., Edmonds P., Sly L. I., Brenner D. J. 1989; Campylobacter mustelae, a new species resulting from the elevation of Campylobacter pylori subsp. mustelae to species status. Int. J. Syst. Bacteriol. 39:301–303
    [Google Scholar]
  15. Fox J. G., Edrise B. M., Cabot E. B., Beaucage C., Murphy J. C., Prostak K. S. 1986; Campylobacter-\i\ae organisms isolated from the gastric mucosa of ferrets. Am. J. Vet. Res. 47:236–239
    [Google Scholar]
  16. Fox J. G., Paster B. P., Lee A., Dewhirst F. E., Taylor N. S. 1990; Genus and species probes for identification of Helicobacter sp. from man and animals. Third Workshop of the European Helicobacter pylori Study Group. Rev. Esp. Inf. Dig. (Suppl. 1) 78:30–31
    [Google Scholar]
  17. Goodwin C. S., Armstrong J. A., Chilvers T., Peters M., Collins M. D., Sly L., McConnell W., Harper W. E. S. 1989; Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter pylori gen. nov. and Helicobacter mustelae comb, nov., respectively. Int. J. Syst. Bacteriol. 39:397–405
    [Google Scholar]
  18. Goodwin C. S., Armstrong J. A., Marshall B. J. 1986; Campylobacterpyloridis, gastritis and peptic ulceration. J. Clin. Pathol. 39:353–365
    [Google Scholar]
  19. Goodwin C. S., McCulloch R. K., Armstrong J. A., Wee S. H. 1985; Unusual cellular fatty acids and distinctive ultrastructure in a new spiral bacterium (Campylobacter pyloridis) from the human gastric mucosa. J. Med. Microbiol. 19:257–267
    [Google Scholar]
  20. Heneine N., Lee A. Unpublished data.
  21. Henry G. A., Long P. H., Burns J. L., Charbonneau D. L. 1987; Gastric spirillosis in beagles. Am. J. Vet. Res. 48:831–836
    [Google Scholar]
  22. Kirkbride C. A., Gates C. E., Collins J. E., Ritchie A. E. 1985; Ovine abortion associated with an anaerobic bacterium. J. Am. Vet. Med. Assoc. 186:789–791
    [Google Scholar]
  23. Kupper J., Wildhaber I., Gao Z., Baeverlin E. 1989; Basal-body-associated disks are additional structural elements of the flagellum apparatus isolated from Wolinella succinogenes. J. Bacteriol. 171:2803–2810
    [Google Scholar]
  24. Lee A. 1984; Neglected niches. The microbial ecology of the gastrointestinal tract. Adv. Microb. Ecol. 8:115–162
    [Google Scholar]
  25. Lee A., Hazell S. L., O’Rourke J. L., Kouprach S. 1988; Isolation of a spiral shaped bacterium from the cat stomach. Infect. Immun. 56:2843–2850
    [Google Scholar]
  26. Lim R. K. S. 1920; A parasitic spiral organism in the stomach of the cat. Parasitology 12:108–113
    [Google Scholar]
  27. Lockard V. G., Boler R. K. 1970; Ultrastructure of a spiraled microorganism in the gastric mucosa of dogs. Am. J. Vet. Res. 31:1453–1462
    [Google Scholar]
  28. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  29. Marshall B. J. 1986; Campylobacter pyloridis and gastritis. J. Infect. Dis. 153:650–657
    [Google Scholar]
  30. Marshall B. J., McGechie D. B., Rogers P. A., Glancy R. J. 1985; Pyloric Campylobacter infection and gastroduodenal disease. Med. J. Aust. 142:439–444
    [Google Scholar]
  31. Marshall B. J., Royce H., Annear D. I., Goodwin C. S., Pearman J. W., Warren J. R., Armstrong J. A. 1984; Original isolation of Campylobacter pyloridis from human gastric mucosa. Microbios Lett. 25:83–88
    [Google Scholar]
  32. O’Rourke J. L., Lee A., Fox J. G. An ultrastructural study of Helicobacter mustelae: evidence of a specific association with gastric mucosa. J. Med. Microbiol. in press
    [Google Scholar]
  33. Paster B. J., Dewhirst F. E. 1988; Phylogeny of Campylobacters, wolinellas, Bacteroides gracilis, and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int. J. Syst. Bacteriol. 38:56–62
    [Google Scholar]
  34. Paster B. P., Lee A., Fox J. G., Dewhirst F. E., Tordoff L. A., Fraser J. G., O’Rourke J. L., Taylor N. S., Ferrero R. 1991; Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae, and related bacteria. Int. J. Syst Bacteriol. 41:31–38
    [Google Scholar]
  35. Phillips M. W., Lee A. 1983; Isolation and characterization of a spiral bacterium from the crypts of rodent gastrointestinal tracts. Appl. Environ. Microbiol. 45:675–683
    [Google Scholar]
  36. Queiroz D. M. M., Contigli C., Comibra R. S., Nogueira A. M. M. F., Mendes E. N., Rocha G. A., Silva R. J. A. 1990; Mice stomach naturally colonised by a spiral bacterium. Third Workshop of the European Helicobacter pylori Study Group. Rev. Esp. Inf. Dig. (Suppl. 1) 78:52–53
    [Google Scholar]
  37. Romaniuk P. J., Zoltowska B., Trust T. J., Lane D. J., Olsen G. J., Pace N. R., Stahl D. A. 1987; Campylobacter pylori, the spiral bacterium associated with human gastritis, is not a true Campylobacter sp. J. Bacteriol. 169:2137–2141
    [Google Scholar]
  38. Romero S., Archer J. R., Hamacher M. E., Bologna S. M., Schell R. F. 1988; Case report of an unclassified microaerophilic bacterium associated with gastroenteritis. J. Clin. Microbiol. 26:142–143
    [Google Scholar]
  39. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  40. Savage D. C., McAllister J. S., Davies C. P. 1971; Anaerobic bacteria on the mucosal epithelium of the murine large bowel. Infect. Immun. 4:492–502
    [Google Scholar]
  41. Seymour C., Kim M., Paster B. J., Dewhirst F. E., Fox J. G. 1991 Helicobacter, Campylobacter, Yersinia, Budvicia, and Giardia from mammal and bird feces. 324 Abstr. 91st Gen. Meet. Am. Soc. Microbiol 1991 American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  42. Sly L. L, Blackall L. L., Kraat P. C., Tao T.-S., Sangkhobol V. 1986; The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J. Microbiol. Methods 5:139–156
    [Google Scholar]
  43. Swan M. A. 1985; Electron microscopic observations of structures associated with the flagella of Spirillum volutans. J. Bacteriol. 161:1137–1145
    [Google Scholar]
  44. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. 1991; Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int. J. Syst Bacteriol. 41:88–103
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-42-1-27
Loading
/content/journal/ijsem/10.1099/00207713-42-1-27
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error