1887

Abstract

In 1979 and 1980, more than 400 harbor seals () along the New England coast of the United States died of epizootic pneumonia that was attributed to an influenza virus. Six mycoplasma isolates that were recovered from the respiratory tracts of affected seals were investigated and were found to be serologically identical and distinct from previously described species. These isolates required serum for growth, did not possess a cell wall, and did not hydrolyze urea. Arginine was hydrolyzed, glucose was not fermented, film and spots were observed on horse serum agar, phosphatase was produced, tetrazolium was not reduced, and serum and casein were not digested. The guanine-plus-cytosine content of the DNA was 27.8 mol%. We propose the name for these isolates. The type strain of is strain 105 (= ATCC 33657).

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1992-04-01
2024-04-27
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References

  1. Alubtto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–38
    [Google Scholar]
  2. Barker I. K., Geraci J. R. D. St, Aubin L. Ruhnke, Gagnon A. N., Lang G., Baker A. S. 1980; Pathology of epizootic pneumonia in harbour seals (Phoca vitulina) associated with influenza A virus and Mycoplasma infection. Abstr. 26Wildl. Dis. Assoc. Annu. Meet.Baton Rouge, La
    [Google Scholar]
  3. Clyde W. A. 1964; Mycoplasma species identification based on growth inhibition by specific antisera. J. Immunol. 92:958–965
    [Google Scholar]
  4. Deines L., Bullivant S. 1968; Morphology and reproductive processes of the L-forms of bacteria. II. Comparative study of L-forms and Mycoplasma with the electron microscope. J. Bacteriol. 95:672–687
    [Google Scholar]
  5. Del Giudice R. A., Robillard F., Carski T. R. 1967; Immunofluorescence identification of mycoplasma on agar by use of incident illumination. J. Bacteriol. 93:1205–1209
    [Google Scholar]
  6. Flossdorf J. 1983; A rapid method for the determination of base composition of bacterial DNA. J. Microbiol. Methods 1:305–311
    [Google Scholar]
  7. Freundt E. A., Andrews B. E., Emo H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium-poly-anetholsulfonate and digitonin. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 225:104–112
    [Google Scholar]
  8. Geraci J. R., Barker I. K., Aubin D. J. St, Webster R. G. 1981; Epizootic pneumonia of harbour seals (Phoca vitulina),. 186–187 Wildl. Dis. Proc. 4th Int. Conf. Wildl. Dis. Assoc
    [Google Scholar]
  9. Geraci J. R., Aubin D. J. St, Barker I. K., Hinshaw V. S., Webster R. G., Ruhnke H. L. 1984; Susceptibility of grey (Halichoerus grypus) and harp (Phoca groenlandica) seals to the influenza virus and Mycoplasma of epizootic pneumonia of harbor seals (Phoca vitulina’).. Can. J. Fish. Aquat. Sci. 41:151–156
    [Google Scholar]
  10. Geraci J. R., Aubin D. J. St, Barker I. K., Webster R. G., Hinshaw V. S., Bean W. J., Ruhnke H. L., Prescott J. H., Early G., Baker A. S., Madoff S., Schooley R. T. 1982; Mass mortality of harbor seals: pneumonia associated with influenza A virus. Science 215:1129–1131
    [Google Scholar]
  11. Giebel J., Meier J., Binder A.J., Flossdorf J., Poveda J. B., Schmidt R., Kirchhoff H. 1991; Mycoplasma phocarhinis sp. nov. and Mycoplasma phocacerebrale sp. nov. two new species from harbor seals (Phoca vitulina L.). Int. J. Syst. Bacteriol. 41:39–44
    [Google Scholar]
  12. Hayflick L. 1965; Tissue cultures and mycoplasmas. Tex. Rep. Biol. Med. 23:285–303
    [Google Scholar]
  13. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes. 1979; Proposal of minimal standards for the description of new species of the class Mollicutes.. Int. J. Syst. Bacteriol. 29:172–180
    [Google Scholar]
  14. Madoff S., Schooley R. T., Ruhnke H. L., Del Giudice R. A., Barker I. K., Geraci J., Baker A. S. 1982; Mycoplasmal pneumonia in phocid (harbor) seals. Rev. Infect. Dis. 4:S241
    [Google Scholar]
  15. Rosendal S., Black F. T. 1972; Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies. Acta Pathol. Microbiol. Scand. Sect. B 80:615–622
    [Google Scholar]
  16. Ruhnke H. L., Doig P. A., MacKay A. L., Gagnon A., Kierstead M. 1978; Isolation of ureaplasma from bovine granular vulvitis. Can. J. Comp. Med. 42:151–155
    [Google Scholar]
  17. Ruhnke H. L., Palmer N. C., Doig P. A., Miller R. B. 1984; Bovine abortion and neonatal death associated with Ureaplasma diversum.. Theriogenology 21:295–301
    [Google Scholar]
  18. Smith A. W., Prato C. M., Gilmartin W. G., Brown R. J., Keyes M. C. 1974; A preliminary report on potentially pathogenic microbiological agents recently isolated from pinnipeds. J. Wildl. Dis. 10:54–59
    [Google Scholar]
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