1887

Abstract

On the basis of the idea that DNA sequences encoding cell surface-exposed regions of outer membrane proteins are genus or species specific, two oligonucleotide probes which were based on the PhoE protein of were evaluated. In slot blot hybridizations and in polymerase chain reactions, no cross-hybridizations were observed with non- strains. When the probes were tested on 75 different K-antigen reference strains, 16 strains were not recognized although they did produce PhoE protein under phosphate starvation. To determine whether these 16 strains belong to (a) different species, the reference strains were also tested for the ability to produce indole and to grow at 10°C and their whole-cell fatty acid patterns were analyzed by gas chromatography. A strong correlation was observed among (i) reaction with the probes, (ii) the inability to produce indole, (iii) the inability to grow at 10°C, and (iv) the presence of the hydroxylated fatty acid C, From these results we conclude that the two oligonucleotides are specific for the species Furthermore, analysis of fatty acid patterns appears to be a useful tool to distinguish from other species.

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1992-04-01
2024-03-28
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References

  1. Bagley S. T., Seidler R. J., Brenner D. J. 1981; Klebsiella planticola sp. nov.: a new species of Enterobacteriaceae found primarily in nonclinical environments. Curr. Microbiol. 6:105–109
    [Google Scholar]
  2. Bascomb S., Lapage S. P., Willcox W. R., Curtis M. A. 1971; Numerical classification of the tribe Klebsielleae. J. Gen. Microbiol. 66:279–295
    [Google Scholar]
  3. Frobisher M. 1957 Fundamentals of microbiology, 6th.242 The W. B. Saunders Co.; Philadelphia:
    [Google Scholar]
  4. Hofstra H., Dankert J. 1980; Major outer membrane proteins: common antigens in Enterobacteriaceae species. J. Gen. Microbiol. 119:123–131
    [Google Scholar]
  5. Izard D., Gavini F., Leclerc H. 1980; Polynucleotide sequence relatedness and genome size among Enterobacter intermedium sp. nov. and the species Enterobacter cloacae and Klebsiella pneumoniae.. Zentralbl. Bakteriol Abt. 1 Orig. C 1:51–60
    [Google Scholar]
  6. Lechevalier M. P. 1977; Lipids in bacterial taxonomy—a taxonomist’s view. Crit. Rev. Microbiol. 5:109–210
    [Google Scholar]
  7. Levinthal C., Signer E. R., Fetherolf K. 1962; Reaction and hybridization of reduced alkaline phosphatase. Proc. Natl. Acad. Sci. USA 48:1230–1237
    [Google Scholar]
  8. Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. 1975; Electrophoretic resolution of the “major outer membrane protein” of Escherichia coli K-12 into four bands. FEBS Lett. 58:254–258
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratoiy; Cold Spring Harbor, N.Y:
    [Google Scholar]
  10. Miller I., Berger T. 1985 Hewlett-Packard application note. 228–241 Hewlett-Packard Co.; Palo Alto, Calif:
    [Google Scholar]
  11. Mori M., Ohta M., Agata N., Kido N., Arakawa Y., Ito H., Komatsu T., Kato N. 1989; Identification of species and capsular types of Klebsiella clinical isolates, with special reference to Klebsiella planticola. Microbiol. Immunol. 33:887–895
    [Google Scholar]
  12. Orskov I. 1984 Genus V. Klebsiella Trevisan 1885, 105AL. 461–465 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  13. Ørskov I. Personal communication
  14. Ørskov I., Ørskov F. 1984; Serotyping of Klebsiella.. Methods Microbiol. 14:143–164
    [Google Scholar]
  15. Overbeeke N., Lugtenberg B. 1980; Expression of outer membrane protein e of Escherichia coli K-12 by phosphate limitation. FEBS Lett. 112:229–232
    [Google Scholar]
  16. Sato T., Yura T. 1979; Chromosomal localization and expression of the structural gene for major outer membrane protein la of Escherichia coli K-12 and of the homologous gene of Salmonella typhimurium.. J. Bacteriol. 139:468–477
    [Google Scholar]
  17. Spierings G., Hofstra H., Huis in’t Veld J., Hoekstra W., Tommassen J. 1989; Development of enterobacterium-specific oligonucleotide probes based on the surface-exposed regions of outer membrane proteins. Appl. Environ. Microbiol. 55:3250–3252
    [Google Scholar]
  18. Tommassen J. 1988 Biogenesis and topology of outer membrane proteins in Escherichia coli,. 351–373 Op den Kamp J. A. F.ed Membrane biogenesis. NATO ASI series H16 Springer-Verlag KG; Berlin:
    [Google Scholar]
  19. Tommassen J., Lugtenberg B. 1980; Outer membrane protein e of Escherichia coli K-12 is co-regulated with alkaline phosphatase. J. Bacteriol. 143:151–157
    [Google Scholar]
  20. Tommassen J., Overduin P., Lugtenberg B., Bergmans H. 1982; Cloning of phoE, the structural gene for the Escherichia coli phosphate limitation-inducible outer membrane pore protein. J. Bacteriol. 149:668–672
    [Google Scholar]
  21. Tommassen J., van Tol H., Lugtenberg B. 1983; The ultimate localization of an outer membrane protein of Escherichia coli K-12 is not determined by the signal sequence. EMBO J. 2:1275–1279
    [Google Scholar]
  22. Van der Ley P., Bekkers A., van Meersbergen J., Tommassen J. 1987; A comparative study on the phoE genes of three enterobacterial species. Implications for structure-function relationships in a pore-forming protein of the outer membrane. Eur. J. Biochem. 164:469–475
    [Google Scholar]
  23. Van der Ley P., Struyve M., Tommassen J. 1986; Topology of outer membrane pore protein phoE of Escherichia coli.. J. Biol. Chern. 261:12222–12225
    [Google Scholar]
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