1887

Abstract

DNA reassociation was used to determine levels of relatedness among four thermophilic strains that are able to use formate and between these organisms and two representative strains of , strain ▵H (= DSM 1053 = ATCC 29096) (T = type strain) and strain Marburg (= DSM 2133). Three homology groups were delineated, and these groups coincided with the clusters identified by antigenic fingerprinting. The first group, which had levels of cross hybridization that ranged from 73 to 99%, included ▵H, Z-245, sp. strain THF, and sp. strain FTF. The second and third groups were each represented by only one strain, sp. strain CB-12 and Marburg, respectively (cross-hybridization levels, 13 to 30 and 29 to 33%, respectively). Our results indicate that the name should be rejected as it is a synonym of . The taxonomic positions of strains Marburg and CB-12 need further investigation.

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1992-07-01
2024-04-26
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References

  1. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: réévaluation of a unique biological group. Microbiol. Rev. 43:260–296
    [Google Scholar]
  2. Benstead J., Archer D. B., Lloyd D. 1991; Formate utilization by members of the genus Methanobacterium. Arch. Microbiol. 156:34–37
    [Google Scholar]
  3. Bezrukova L. V., Obraztsova A. Y., Zhilina T. N. 1989; Immunological studies on a group of methane-producing bacterial strains. Microbiology (Engl. Transi. Mikrobiologiya) 58:77–83
    [Google Scholar]
  4. Brandis A., Thauer R. K., Stetter K. O. 1981; Relatedness of strains AH and Marburg of Methanobacterium thermoautotrophicum. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe C 1:311–317
    [Google Scholar]
  5. Brandis-Heep A. 1981; Spezies-Characterizierung von Methanobacterium thermoautotrophicum (Marburg) und Desulfovibrio vulgaris (Marburg). Ph.D. thesis Philipps-Universität Marburg/Lahn; Marburg, Germany:
    [Google Scholar]
  6. Chumakov K. M., Zhilina T. N., Zvyagintseva I. S., Tarasov A. T., Zavarzin G. A. 1987; 5S RNA in archaebacteria. Zh. Obshch. Biol. 48:167–191 In Russian.
    [Google Scholar]
  7. Ferragut C., Leclerc H. 1976; Etude comparative des méthodes de détermination du Tm de l’ADN bactérien. Ann. Microbiol. (Paris) 127A:223–235
    [Google Scholar]
  8. Fuchs G., Stupperich E., Thauer R. K. 1978; Acetate assimilation and the synthesis of alanine, aspartate and glutamate in Methanobacterium thermoautotrophicum. Arch. Microbiol. 117:61–66
    [Google Scholar]
  9. International Journal of Systematic Bacteriology 1986; Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List no. 21. Int. J. Syst. Bacteriol. 36:489
    [Google Scholar]
  10. Johnson J. L. 1981 Genetic characterization. 450–472 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  11. Macario A. J. L., Conway de Macario E. 1983; Antigenic fingerprinting of methanogenic bacteria with polyclonal antibodies probes. Syst. Appl. Microbiol. 4:451–458
    [Google Scholar]
  12. Macario A. J. L., Conway de Macario E. 1985; Antibodies for methanogenic biotechnology. Trends Biotechnol. 3:204–208
    [Google Scholar]
  13. Macario A. J. L., Conway de Macario E. 1985 Monoclonal antibodies of predefined molecular specificity for identification and classification of methanogens and for probing their ecological niches. 213–247 Macario A. J. L., Conway de Macario E.ed Monoclonal antibodies against bacteria 2 Academic Press, Inc.; Orlando, Fla:
    [Google Scholar]
  14. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y:
    [Google Scholar]
  15. Mitchell R. M., Loeblich L. A., Klotz L. C., Loeblich A. R. III 1979; DNA organization in Methanobacterium thermoautotrophicum. Science 204:1082–1084
    [Google Scholar]
  16. Ndlling J., de Vos W. M. Unpublished data.
  17. Ndlling J., Frijlink M., de Vos W. M. 1991; Isolation and characterization of plasmids from different strains of Methanobacterium thermoformicicum. J. Gen. Microbiol. 137:1981–1986
    [Google Scholar]
  18. Ndlling J., Hahn D., Schleifer K. H., de Vos W. M. Unpublished data.
  19. Priest F. G., Goodfellow M., Shute L. A., Berkeley R. C. W. 1987; Bacillus amyloliquefaciens sp. nov., nom. rev. Int. J. Syst. Bacteriol. 37:69–71
    [Google Scholar]
  20. Touzel J. P., Petroff D., Maestrojuan G. M., Prensier G., Albagnac G. 1988; Isolation and characterization of a thermophilic Methanobacterium able to use formate, the strain FTF. Arch. Microbiol. 149:291–296
    [Google Scholar]
  21. Yamamoto K., Tachibana A., Dhavises G., Tanaka T., Takiguchi M., Oi S. 1989; Characterization of a thermophilic formate-utilizing methanogen, Methanobacterium thermoformicicum strain SF-4. Agric. Biol. Chern. 53:533–534
    [Google Scholar]
  22. Zeikus J. G. 1983; Metabolism of one-carbon compounds by chemotrophic anaerobes. Adv. Microb. Physiol. 38:215–297
    [Google Scholar]
  23. Zeikus J. G., Wolfe R. S. 1972; Methanobacterium thermoautotrophicus sp. n., an anaerobic, autotrophic, extreme thermophile. J. Bacteriol. 109:707–713
    [Google Scholar]
  24. Zhao Y., Zhang H., Boone D., Mah R. A. 1986; Isolation and characterization of a fast-growing, thermophilic Methanobacterium species. Appl. Environ. Microbiol. 52:1227–1229
    [Google Scholar]
  25. Zhilina T. N., Ilarionov S. A. 1984; Isolation and comparative characteristics of methanogenic bacteria assimilating formate with the description of Methanobacterium thermoformicicum sp. nov. Mikrobiologiya 53:785–790
    [Google Scholar]
  26. Zhilina T. N., Ilarionov S. A. 1985; Characteristics of formate-assimilating methane bacteria and description of Methanobacterium thermoformicicum sp. nov. Microbiology (Engl. Transl. Mikrobiologiya) 53:647–651
    [Google Scholar]
  27. Zinder S. H., Koch M. 1984; Nonaceticlastic methanogenesis from acetate: acetate oxidation by a thermophilic syntrophic coculture. Arch. Microbiol. 138:263–272
    [Google Scholar]
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