- International Journal of Systematic and Evolutionary Microbiology
- Volume 42, Issue 3
f Characterization of Three Thermophilic Strains of Methanothrix (“Methanosaeta”) thermophila sp. nov. and Rejection of Methanothrix (“Methanosaeta”) thermoacetophila
- Authors: Yoichi Kamagata*, Hiroko Kawasaki, Hiroshi Oyaizu, Kazunori Nakamura, Eiichi Mikami, Ginro Endo, Yosuke Koga, Kazuhide Yamasato
- *Corresponding author.
- Int J Syst Evol Microbiol, July 1992 42: 463-468, doi: 10.1099/00207713-42-3-463
- Subject: Original Papers Relating To Systematic Bacteriology
- Published Online:
Three thermophilic Methanothrix (“Methanosaeta”) strains, strains PT T (= DSM 6194T) (T = type strain), CALS-1 (= DSM 3870), and Z-517 (= DSM 4774), were characterized chemotaxonomically and compared with five mesophilic strains, Methanothrix soehngenii (“Methanosaeta concilii”) GP6 (= DSM 3671), Opfikon (= DSM 2139), FE (= DSM 3013), UA, and PM. These methanogens were exclusively acetotrophic and had a characteristic sheathed structure. The DNA base compositions of the strains which we studied ranged from 50.3 to 54.3 mol% guanine plus cytosine. The thermophilic strains often had phase-refractive gas vesicles inside their cells. Denaturing electrophoresis of proteins showed that the mesophilic and thermophilic Methanothrix strains formed two distinct groups and that there were differences in protein patterns between the groups. The difference between the thermophiles and mesophiles was also verified by comparing partial 16S rRNA sequences (ca. 30 base differences in ca. 540 bases). On the basis of our results, we propose the name Methanothrix thermophila for the three thermophilic strains. The type strain of M. thermophila is strain PT (= DSM 6194). We also propose that the name Methanothrix thermoacetophila (“Methanosaeta thermoacetophila”), which was given to strain Z-517 (type strain), should be rejected because of its description, which was based on an enrichment culture, was inadequate.
Copyright © 1992 International Union of Microbiological Societies | Published by the Microbiology Society
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