1887

Abstract

The protein patterns of whole cells of and were studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein patterns of the 16 strains of and studied, including the type strains of these two species, resembled each other, except that there were 71-, 41-, 34-, and 26-kDa proteins in the pattern and 74-, 44-, 36-, and 25-kDa proteins in the pattern. This observation indicates that there is some phenotypic heterogeneity in the genus In addition, the protein patterns of serotype reference strains representing serotypes 1 through 23 and type N were compared. The protein patterns of serotype 1a, 1b, 2, 4, 5, 6, 8, 9, 11, 12, 15, 16, 19, and 21 and type N strains were similar to the pattern of the type strain of (strain ATCC 19414). Conversely, the protein patterns of serotype 3, 7, 10, 14, and 20 strains were very similar to the pattern of the type strain of (strain ATCC 43339). An atypical pattern was observed in serotype 13, 17, 18, 22, and 23 strains. These results suggest that this method may be used as an aid in studying the taxonomy of these bacteria.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-43-1-111
1993-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/43/1/ijs-43-1-111.html?itemId=/content/journal/ijsem/10.1099/00207713-43-1-111&mimeType=html&fmt=ahah

References

  1. Galan J.E., Timoney J. F. 1990; Cloning and expression in Escherichia coli of a protective antigen of Erysipelothrix rhusiopathiae. Infect. Immun. 58:3116–3121
    [Google Scholar]
  2. Jones D. 1986; Genus Erysipelothrix. p. 1245–1249 In Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 2 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  3. Kalf G., White T. G. 1963; The antigenic components of Erysipelothrix rhusiopathiae. II. Purification and chemical characterization of a type-specific antigen. Arch. Biochem. Biophys 102:39–47
    [Google Scholar]
  4. Lachmann P. G., Deicher H. 1986; Solubilization and characterization of surface antigenic components of Erysipelothrix rhusiopathiae T28. Infect. Immun. 52:818–822
    [Google Scholar]
  5. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
    [Google Scholar]
  6. Ndrrung V., Munch B., Errebo Larsen H. 1987; Occurrence, isolation and serotyping of Erysipelothrix rhusiopathiae in cattle and pig slurry. Acta Vet. scand. 28:9–14
    [Google Scholar]
  7. Razin S., Rottem S. 1967; Identification of Mycoplasma and other microorganisms by polyacrylamide gel electrophoresis of cell proteins. J. Bacteriol. 94:1807–1810
    [Google Scholar]
  8. Takahashi T., Fujisawa T., Benno Y., Tamura Y., Sawada T., Suzuki S., Muramatsu M., Mitsuoka T. 1987; Erysipelothrix tonsillarum sp. nov. isolated from tonsils of apparently healthy pigs. Int. J. Syst. Bacteriol. 37:166–168
    [Google Scholar]
  9. Takahashi T., Fujisawa T., Tamura Y., Suzuki S., Muramatsu M., Sawada T., Benno Y., Mitsuoka T. 1992; DNA relatedness among Erysipelothrix rhusiopathiae strains representing all twenty-three serovars and Erysipelothrix tonsillarum.. Int. J. Syst. Bacteriol. 42:469–473
    [Google Scholar]
  10. Takahashi T., Sawada T., Muramatsu M., Tamura Y., Fujisawa T., Benno Y., Mitsuoka T. 1987; Serotype, antimicrobial susceptibility, and pathogenicity of Erysipelothrix rhusiopathiae isolates from tonsils of apparently healthyslaughter pigs. J. Clin. Microbiol. 25:536–539
    [Google Scholar]
  11. Takahashi T., Sawada T., Takagi M., Seto K., Kanzaki M., Maruyama T. 1984; Serotypes of Erysipelothrix rhusiopathiae strains isolated from slaughter pigs affected with chronic erysipelas. Jpn. J. Vet. sci. 46:149–153
    [Google Scholar]
  12. Towbin H. T., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and applications. Proc. Natl. Acad. sci. USA 76:4350–4354
    [Google Scholar]
  13. White T. G., Mirikitani F. K. 1975; Some biological and physical-chemical properties of Erysipelothrix rhusiopathiae.. Cornell Vet. 66:152–163
    [Google Scholar]
  14. Wood R. L. 1984; Swine erysipelas: a review of prevalence and research. J. Am. Vet. Med. Assoc. 184:944–948
    [Google Scholar]
  15. Wood R. L., Shuman R. D. 1975; Swine erysipelas. p. 565–620 In Dunne H. W., Leman A. D. (ed.) Diseases of swine, 4th ed.. Iowa State University Press; Ames:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-43-1-111
Loading
/content/journal/ijsem/10.1099/00207713-43-1-111
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error