1887

Abstract

Four strains of a novel species were isolated from the stomachs of cheetahs ( ) with gastritis. These isolates were phenotypically similar to The isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37°C, but not at 25 or 42°C, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. The isolates did not ferment glucose, mannitol, inositol, sorbitol, rhamnose, sucrose, melibiose, amygdalin, or arabinose; hydrolyze hippurate or indoxyl acetate; or reduce nitrate. They did not produce HS from triple sugar iron agar, and they did not grow in the presence of 1.0% glycine or 1.5% NaCl. They were resistant to nalidixic acid and sensitive to cephalothin and metronidazole. Cells were typically 0.3 by 2.0 μm and possessed tufts of two to five sheathed, monopolar flagella. The G+C content of strain 90-119 was 30 mol%. Cluster analysis of densitometry scans of polyacrylamide protein gels revealed more than 70% similarity of the cheetah isolates to , less than 60% similarity to and less than 50% similarity to Complete 16S rRNA sequences were determined for two of the cheetah isolates. Phylogenetic analysis was performed by comparing the cheetah sequences to those of 19 reference strains, inluding (two strains), subsp. , a sp. (pig isolate), , and The 16S rRNA sequences for 13 of the 19 reference species have not previously been reported. Phylogenetic analysis demonstrated that the cheetah isolates were most closely related to (97.4% similarity), (96.1% similarity), and (93.4% similarity). On the basis of these findings, we propose that these isolates represents a novel species of which we designate The type strain is 90-119 (CCUG 29263, ATCC 51101).

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1993-01-01
2024-03-29
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References

  1. Archer J. R., Romero S., Ritchie A. E., Hamacher M. E., Steiner B. M., Bryner J. H., Scheel R. F. 1988; Characterization of an unclassified microaerophilic bacterium associated with gastroenteritis. J. Clin. Microbiol 26:101–105
    [Google Scholar]
  2. Bronsdon M. A., Goodwin C. S., Sly L. I., Chilvers T., Schoenknecht F. D. 1991; Helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (Macaca nemestrina ). Int. J. Syst. Bacteriol 41:148–153
    [Google Scholar]
  3. Brosius J., Palmer M. L, Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc. Natl. Acad. Sci. USA 75:4801–4808
    [Google Scholar]
  4. Bryner J. H. 1988; Flexispira rappini. gen. nov., sp. nov. A motile, urease-producing rod similar to Campylobacter pyloridis. p. 440–442 In Kaijser B., Falsen E. (ed.) Proceedings of the Fourth International Workshop on Campylobacter Infections, Goteborg, Sweden Goterna, Kungalv, Sweden:
    [Google Scholar]
  5. Dewhirst F. E., Paster B. J. 1992 Unpublished data
  6. Eaton K. A., Radin M. J., Kramer L., Wack R., Sherding R., Krakowka S., Morgan D. R. 1991; Gastric spiral bacilli in captive cheetahs. Scand. J. Gastroenterol 26: (Suppl. 181) 38–42
    [Google Scholar]
  7. Edwards P. R., Ewing W. H. 1972; Identification of Enterobacteriaceae. , 3rd ed. Burgess Publishing Co; Minneapo-lis:
    [Google Scholar]
  8. Fennell C. L., Totten P. A., Quinn T. C., Patton D. L., Holmes K. K., Stamm W. E. 1984; Characterization of campylobacter-like organisms isolated from homosexual. men. J. Infect. Dis 149:58–66
    [Google Scholar]
  9. Fox J. G., Cabot E. B., Taylor N. S., Laraway R. 1988; Gastric colonization by Campylobacter pylori subsp mustelae in ferrets. Infect. Immun 56:2994–2996
    [Google Scholar]
  10. Fox J. G., Chilvers T., Goodwin C. S., Taylor N. S., Edmonds P., Sly L. I., Brenner D. J. 1989; Campylobacter mustelae, a new species resulting from the elevation of Campylobacter pylori subsp. mustelae to species status. Int. J. Syst. Bacteriol 39:301–303
    [Google Scholar]
  11. Fox J. G., Correa P., Taylor N. S., Lee A., Otto G., Murphy J. C., Rose R. 1990; Helicobacter mustelae associated gastritis in ferrets: an animal model of Helicobacter pylori gastritis in humans. Gastroenterology 99:352–361
    [Google Scholar]
  12. Fox J. G., Lee A., Otto G., Taylor N. S., Murphy J. C. 1991; Helicobacter felis gastritis in gnotobiotic rats: an animal model of Helicobacter pylori gastritis. Infect. Immun 59:785–791
    [Google Scholar]
  13. Fox J. G., Paster B. J., Dewhirst F. E., Taylor N. S., Yan L.-L., Macuch P. J., Chmura L. M. 1992; Helicobacter mustelae isolation from feces of ferrets: evidence to support fecal-oral transmission of a gastric helicobacter. Infect. Immun 60:606–611
    [Google Scholar]
  14. Goodwin C. S., Armstrong J. A., Chilvers T., Peters M., Collins M. D., Sly L., McConnell W., Harper W. E. S. 1989; Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter pylori and Helicobacter mustelae comb. nov., respectively. Int. J. Syst. Bacteriol 39:397–405
    [Google Scholar]
  15. Hodge D. S., Borczyk A., Wat L.-L. 1990; Evaluation of the indoxyl acetate hydrolysis test for differentiation of Campylobacters. J. Clin. Microbiol 28:1482–1483
    [Google Scholar]
  16. Hunble M. W., King A., Phillips I. 1977; API ZYM: a simple rapid system for the detection of bacterial enzymes. J. Clin. Pathol 30:275–277
    [Google Scholar]
  17. Hwang M.-N., Ederer G. M. 1975; Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. J. Clin. Microbiol 1:114–115
    [Google Scholar]
  18. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. p. 21–132 In Munro H. N. (ed.) Mammalian protein metabolism vol 3 Academic Press, Inc; New York:
    [Google Scholar]
  19. Krakowka S., Morgan D. R., Kraft W. G., Leunk R. D. 1987; Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. Infect. Immun 55:2789–2796
    [Google Scholar]
  20. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc. Natl. Acad. Sci. USA 82:6955–6959
    [Google Scholar]
  21. Lee A., Fox J. G., Otto G., Murphy J. C. 1990; A small animal model of human Helicobacter pylori active chronic gastritis. Gastroenterology 99:1315–1323
    [Google Scholar]
  22. Lee A., Hazell S. L., O’Rourke J., Kouprach S. 1988; Isolation of a spiral-shaped bacterium from the cat stomach. Infect. Immun 56:2843–2850
    [Google Scholar]
  23. Lee A., Krakowka S., Fox J. G., Otto G., Murphy J. C. 1992; Helicobacter felis as a cause of lymphoreticular hyperplasia in the dog stomach. Vet. Pathol in press
    [Google Scholar]
  24. Lee A., Phillips M. W., O’Rourke J. L., Paster B. J., Dewhirst F. E., Fraser G. J., Fox J. G., Sly L. I., Romaniuk P. J., Trust T. J., Kouprach S. 1992; Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. Int. J. Syst. Bacteriol 42:27–36
    [Google Scholar]
  25. Marshall B. J., Warren J. R. 1984; Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet i:1311–1314
    [Google Scholar]
  26. Owen R. J. M., On S. L. W., Hill L. R., Pearson A. D., Morgan D.R. 1989; Strain variation in Campylobacter pylori detected by numerical analysis of one dimensional electro-phoretic protein patterns. Antonie Leeuwenhoek 55:253–268
    [Google Scholar]
  27. Pace B., Matthews E. A., Johnson K. D., Cantor C. R., Pace N. R. 1982; Conserved 5S rRNA complement to tRNA is not required for protein synthesis. Proc. Natl. Acad. Sci. USA 79:36–40
    [Google Scholar]
  28. Paster B. J., Dewhirst F. E. 1988; Phylogeny of Campylobacters, wolinellas, Bacteroides gracilis, and Bacteroides ure- olyticus by 16S ribosomal ribonucleic acid sequencing. Int. J. Syst. Bacteriol 38:56–62
    [Google Scholar]
  29. Paster B. J., Dewhirst F. E., Seymour C., Fraser G. J., Fox J. G. 1991; Helicobacter species isolated from bird and swine feces. Microbiol. Ecol. Health Dis 4: (Special issue):S 107
    [Google Scholar]
  30. Paster B. J., Lee A., Fox J. G., Dewhirst F. E., Tordoff L. A., Fraser G. J., O’Rourke J. L., Taylor N. S., Ferrero R. 1991; Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae, and related bacteria. Int. J. Syst. Bacteriol 41:31–38
    [Google Scholar]
  31. Radin J. M., Eaton K. A., Krakowka S., Morgan D. R., Lee A., Otto G., Fox J. G. 1990; Helicobacter pylori infection in gnotobiotic beagle dogs. Infect. Immun 58:2606–2612
    [Google Scholar]
  32. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4:406–425
    [Google Scholar]
  33. Smibert R. M. 1984; Genus Campylobacter. p. 111–118 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 Williams & Wilkins; Baltimore:
    [Google Scholar]
  34. Tanner A. C. R., Socransky S. S. 1984; Genus Wolinella. p. 646–650 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 Williams & Wilkins; Baltimore:
    [Google Scholar]
  35. Totten P. A., Fennell C. L., Tenover F. C., Wezenberg J. M., Perine P. L., Stamm W. E., Holmes K. K. 1985; Campylobacter cinaedi (sp. nov.) and Campylobacter fennelliae (sp. nov.): two new Campylobacter species associated with enteric disease in homosexual. men. J. Infect. Dis 151:131–139
    [Google Scholar]
  36. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. 1991; Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int. J. Syst. Bacteriol 41:88–103
    [Google Scholar]
  37. Westley J. W., Anderson P. J., Close V. A., Halpern B., Lederberg E.M. 1967; Aminopeptidase profiles of various bacteria. Appl. Microbiol 15:822–825
    [Google Scholar]
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