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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 43, Issue 2

sp. nov. from the Vespid Wasp (Hymenoptera: Vespidae) Free

Abstract

Abstract

Spiroplasma strain MQ-1 (T = type strain) from the hemolymph of the vespid wasp differed serologically from other spiroplasma species, groups, and subgroups. Cells of strain MQ-1 were helical and motile and possessed a single cytoplasmic membrane, with no evidence of a cell wall. The organism grew in conventional mycoplasma medium, in serum fraction, SM-1, MID, and SP-4 liquid media, and on SP-4 solid medium in either aerobic or anaerobic environments. The optimum temperature for growth was 32°C, but multiplication occurred over a wide temperature range (10 to 37°C). The doubling time at 32°C in MID medium was 1.9 h. Strain MQ-1 catabolized glucose but hydrolyzed neither arginine nor urea. Previous work showed that strain MQ-1 has a unique methylase, previously known only in eucaryotes. Also, strain MQ-1 induces production of tumor necrosis factor in bone marrow macrophages. The guanine-plus-cytosine content of the DNA was 28 ± 1 moI%. The genome size of strain MQ-1 was 940 kb (627 MDa); a similar strain, MQ-8, had a genome size of 985 kb (657 MDa). Strain MQ-1 and its allies have the smallest genomes known in the genus Strain MQ-1 (= ATCC 33825) is designated the type strain of a new species, .

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Copyright 1993, International Union of Microbiological Societies
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1993-04-01
2024-04-19
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References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
     [Google Scholar]
  2. Bové J. M., Mouches C., Carle-Junca P., Degorce-Dumas J. R., Tully J. G., Whitcomb R. F. 1983; Spiroplasmas of group I. The Spiroplasma citri cluster. Yale J. Biol. Med. 56:573–582
     [Google Scholar]
  3. Carle P., Saillard C., Bové J. M. 1983; DNA extraction and purification. Methods Mycoplasmol. 1:295–299
     [Google Scholar]
  4. Carle P., Saillard C., Bové J. M. 1983; Determination of guanine plus cytosine content of DNA. Methods Mycoplasmol. 1:301–308
     [Google Scholar]
  5. Carle P., Tully J. G., Whitcomb R. F., Bové J. M. 1989; Size of the spiroplasmal genome and guanine plus cytosine content of spiroplasmal DNA. Zentralbl. Bakteriol. Suppl. 20:889–892
     [Google Scholar]
  6. Charbonneau D. 1984 Ph.D. thesis Cornell University; Ithaca, N.Y.:
     [Google Scholar]
  7. Clark T. B. 1982; Spiroplasmas: diversity of arthropod reservoirs and host-parasite relationships. Science 217:57–59
     [Google Scholar]
  8. Clark T. B., Whitcomb R. F., Tully J. G., Mouches C., Saillard C., Bové J. M., Wroblewski H., Carle P., Rose D. L., Williamson D. L. 1985; Spiroplasma melliferum sp. nov., a new species from the honeybee (Apis mellifera). Int. J. Syst. Bacteriol. 35:296–308
     [Google Scholar]
  9. Davis R. E., Lee I.-M., Worley J. F. 1981; Spiroplasma floricola, a new species isolated from surfaces of flowers of the tulip tree, Liriodendron tulipifera L. Int. J. Syst. Bacteriol. 31:456–464
     [Google Scholar]
  10. Edward D. G. F F. 1947; A selective medium for pleuropneumonia-like organisms. J. Gen. Microbiol. 1:238–243
     [Google Scholar]
  11. Freundt E. A. 1983; Film and spot production. Methods Mycoplasmol. 1:373–374
     [Google Scholar]
  12. Gardella R. S., Del Giudice R. A. 1983; Hemagglutination, hemadsorption, and hemolysis. Methods Mycoplasmol. 1:379384
     [Google Scholar]
  13. Grau O., Laigret F., Carle P., Tully J. G., Rose D. L., Bovl J. M. 1991; Identification of a plant-derived mollicute as a strain of an avian pathogen, Mycoplasma iowae, and its implications for mollicute taxonomy. Int. J. Syst. Bacteriol. 41:473–178
     [Google Scholar]
  14. Guo Y. H., Chen T. A., Whitcomb R. F., Rose D. L., Tully J. G., Williamson D. L., Ye X. D., Chen Y. X. 1990; Spiroplasma chinensis sp. nov. from flowers of Calystegia hederacea in China. Int. J. Syst. Bacteriol. 40:421–425
     [Google Scholar]
  15. Hackett K. J., Clark T. B. 1989; The ecology of spiroplasmas. 113–200 In Whitcomb R. F., Tully J. G. (ed.) The mycoplasmas vol. 5 Academic Press, Inc.; New York:
     [Google Scholar]
  16. International Committee on Systematic Bacteriology Subcommit tee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes. Int. J. Syst. Bacteriol. 29:172–180
     [Google Scholar]
  17. Konai M., Clark E. A., Whitcomb R. F. 1992; Temperature ranges and optima of spiroplasmas. IOM Letters 2:137
     [Google Scholar]
  18. Markham P. G., Clark T. B., Whitcomb R. F. 1983; Culture techniques for spiroplasmas from arthropods. Methods Mycoplasmol. 1:217–223
     [Google Scholar]
  19. Monches C., Bové J. M., Albisetti J., Clark T. B., Tully J. G. 1982; A spiroplasma of serogroup IV causes a May disease-like disorder of honeybees in southwestern France. Microb. Ecol. 8:387–399
     [Google Scholar]
  20. Mouches C., Bové J. M., Tully J. G., Rose D. L., McCoy R. E., Carle-Junca P., Garnier M., Saillard C. 1983; Spiroplasma apis, a new species from the honey bee (Apis mellifera). Ann. Microbiol. (Paris) 134A:383–397
     [Google Scholar]
  21. Nur I., Szyf M., Razin A., Glaser G., Rottem S., Razin S. 1985; Procaryotic and eucaryotic traits of DNA methylation in spiroplasmas (mycoplasmas). J. Bacteriol. 164:19–24
     [Google Scholar]
  22. Saglio P., L’Hospital M., Laflèche D., Dupont G., Bové J. M., Tully J. G., Freundt E. A. 1973; Spiroplasma citri gen. and sp. n.: a mycoplasma-like organism associated with “stubborn” disease of citrus. Int. J. Syst. Bacteriol. 23:191–204
     [Google Scholar]
  23. Sher T., Yamin A., Rottem M. S., Gallily R. 1990; In vitro induction of tumor necrosis factor a, tumor cytolysis, and blast transformation by Spiroplasma membranes. J. Natl. Cancer Inst. 82:1142–1145
     [Google Scholar]
  24. Skripal I. G. 1983; Revival of the name Spiroplasmataceae fam. nov., nom. rev., omitted from the 1980 Approved Lists of Bacterial Names. Int. J. Syst. Bacteriol. 33:408
     [Google Scholar]
  25. Tully J. G. 1983; Tests for digitonin sensitivity and sterol requirements. Methods Mycoplasmol. 1:355–362
     [Google Scholar]
  26. Tully J. G. 1983; Cloning and filtration techniques for myco-plasmas. Methods Mycoplasmol. 1:173–177
     [Google Scholar]
  27. Tully J. G. 1984; Family II. Acholeplasmataceae Edward and Freundt 1979. 775–781 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 Williams and Wilkins; Baltimore:
     [Google Scholar]
  28. Tully J. G., Rose D. L., Clark E., Carle P., Bové J. M., Henegar R. B., Whitcomb R. F., Colflesh D. E., Williamson D. L. 1987; Revised group classification of the genus Spiroplasma (class Mollicutes), with proposed new groups XII to XXIII. Int. J. Syst. Bacteriol. 37:357–364
     [Google Scholar]
  29. Tully J. G., Rose D. L., Yunker C. E., Cory J., Whitcomb R. F., Williamson D. L. 1981; Helical mycoplasmas (spiroplasmas) from Ixodes ticks. Science 212:1043–1045
     [Google Scholar]
  30. Tully J. G., Whitcomb R. F., Rose D. L., Bové J. M. 1982; Spiroplasma mirum, a new species from the rabbit tick (Haemaphysalis leporispalustris). Int. J. Syst. Bacteriol. 32:92–100
     [Google Scholar]
  31. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Etten J. Y., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J. Bacteriol. 171:6455–6467
     [Google Scholar]
  32. Whitcomb R. F. 1983; Culture media for spiroplasmas. Methods Mycoplasmol. 1:147–158
     [Google Scholar]
  33. Whitcomb R. F., Tully J. G., McCawley P., Rose D. L. 1982; Application of the growth inhibition test to Spiroplasma taxonomy. Int. J. Syst. Bacteriol. 32:387–394
     [Google Scholar]
  34. Williamson D. L., Poulson D. F. 1979; Sex ratio organisms (spiroplasmas) of Drosophila. 175–208 In Whitcomb R. F., Tully J. G. (ed.) The mycoplasmas vol. 3 Academic Press, Inc.; New York:
     [Google Scholar]
  35. Williamson D. L., Tully J. G., Whitcomb R. F. 1979; Serological relationships of spiroplasmas as shown by combined deformation and metabolism inhibition tests. Int. J. Syst. Bacteriol. 29:345–351
     [Google Scholar]
  36. Williamson D. L., Tully J. G., Whitcomb R. F. 1989; The genus Spiroplasma. 71–111 In Whitcomb R. F., Tully J. G. (ed.) The mycoplasmas vol. 5 Academic Press, Inc.; New York:
     [Google Scholar]
  37. Williamson D. L., Whitcomb R. F., Tully J. G. 1978; The spiroplasma deformation test. Int. J. Syst. Bacteriol. Curr. Microbiol. 1:203–207
     [Google Scholar]
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Spiroplasma monobiae sp. nov. from the Vespid Wasp Monobia quadridens (Hymenoptera: Vespidae)
Int J Syst Evol Microbiol 43, 256 (1993); https://doi.org/10.1099/00207713-43-2-256
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