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Volume 43, Issue 2

Serologic and Genomic Relatedness of Group VIII and Group XVII Spiroplasmas and Subdivision of Spiroplasma Group VIII into Subgroups Free

Abstract

Spiroplasmas are currently classified in a group system. Criteria for separation of the twenty-four currently designated groups include serologic relatedness, polyacrylamide gel electrophoretic patterns of proteins, guanine-plus-cytosine base ratios, and, in some cases, DNA-DNA homology. The analysis of DNA-DNA homology and serologic data from a large array of strains recently discovered in dipteran insects reveals that group VIII strain EA-1 from a syrphid fly, strain TAAS-1 from a horse fly, and group XVII strain DF-1 from a deer fly belong to a large complex (supergroup) of strains with various degrees of interrelatedness. Strains DF-1 and EA-1 share DNA-DNA homology of 33 to 48% (high-stringency conditions), while strain TAAS-1 shares 42 to 67% homology with DF-1 and EA-1. The strains had temperature optima of 30 to 37°C, but the temperature minima and maxima reflected the geographic region of strain origin. These three strains also share G+C values of about 30 mol%, utilize arginine, and tend to grow in culture to very high titers (10 cells per ml). The helical cells of these strains are smaller than those of other spiroplasmas and readily pass through filter pores of 220 nm. These data support the taxonomic placement of the biotypes represented by strains EA-1, DF-1, and TAAS-1 into one supergroup, group VIII, with subgroups designated as VIII-1, VIII-2, and VIII-3, respectively. It is proposed that group XVII remain vacant.

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Copyright © 1993 International Union of Microbiological Societies
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1993-04-01
2024-05-09
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References

  1. Abalain-Colloc M.-L., Williamson D. L., Carle P., Abalain J. H., Bonnet F., Tully J. G., Konai M., Whitcomb R. F., Bové J. M., Chastel C. 1993; Division of group XVI spiroplasmas into subgroups. Int. J. Syst. Bacteriol. 43:342–346
     [Google Scholar]
  2. Alluotto B. B., Wittler R. G., Williams C. O., Faber J. E. 1970; Standardized bactcriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
     [Google Scholar]
  3. Bové J. M., Mouches C., Carle-Junca P., Degorce-Dumas J. R., Tully J. G., Whitcomb R. F. 1983; Spiroplasmas of group I: the Spiroplasma citri cluster. Yale J. Biol. Med. 56:573–582
     [Google Scholar]
  4. Carle P., Saillard C., Bové J. M. 1983; Determination of guanine-plus-cytosine content of DNA. Methods Mycoplasmol. 1:301–308
     [Google Scholar]
  5. Carle P., Tully J. G., Whitcomb R. F., Bové J. M. 1990; Size of the spiroplasmal genome and guanine plus cytosine content of spiroplasmal DNA. Zentralbl. Bakteriol. 20: (Suppl.) 889–892
     [Google Scholar]
  6. Chastel C, Gilot B., Le Goff F., Devau B., Kerdraraon G., Humphrey-Smith I., Gruffaz R., Simitzix-Le Flohic A.-M. 1990; New developments in the ecology of mosquito spiroplasmas. Zentralbl. Bakteriol. 20: (Suppl.) 455–460
     [Google Scholar]
  7. Chastel C, Gilot B., Le Goff F., Gruffaz R., Abalain-Colloc M.-L. 1985; Isolement de spiroplasmes en France (Savoie, Alpes du Nord) à partir de moustiques du genre Aedes. CR. Acad. Sci. 300:261–266
     [Google Scholar]
  8. Clark T. B. 1982; Spiroplasmas: diversity of arthropod reservoirs and host-parasite relationships. Science 217:57–59
     [Google Scholar]
  9. Clark T. B., Peterson B. V., Whitcomb R. F., Henegar R. B., Hackett K. J., Tully J. G. 1984; Spiroplasmas in the Tabanidae. Isr. J. Med. Sci. 20:1002–1005
     [Google Scholar]
  10. Degorce-Dumas J. R., Ricard B., Bové J. M. 1983; Hybridization between mycoplasma DNAs. Methods Mycoplasmol. 1:319–326
     [Google Scholar]
  11. French F. E. Unpublished data
     [Google Scholar]
  12. French F. E., Whitcomb R. F. Unpublished data
     [Google Scholar]
  13. French F. E., Whitcomb R. F., Tully J. G., Hackett K. J., Clark E. A., Henegar R. B., Wagner A. G., Rose D. L. 1990; Tabanid spiroplasmas of the Southeast USA: new groups, and correlation with host life history strategy. Zentralbl. Bakteriol. 20: (Suppl.) 919–921
     [Google Scholar]
  14. Guo Y. H., Chen T. A., Whitcomb R. F., Rose D. L., Tully J. G., Williamson D. L., Ye X. D., Chen Y. X. 1990; Spiroplasma chinense sp. nov. from flowers of Calystegia hederacea in China. Int. J. Syst. Bacteriol. 40:421–425
     [Google Scholar]
  15. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes 1984; Minutes of the interim meeting, 30 August to 6 September 1982, Tokyo, Japan. Int. J. Syst. Bacteriol. 34:361–365
     [Google Scholar]
  16. Johnson J. L. 1984; Nucleic acids in bacterial classification. 8–11 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 Williams & Wilkins; Baltimore:
     [Google Scholar]
  17. Junca P., Saillard C., Tully J., Garcia-Jurado O., Degorce-Dumas J. R., Mouches C., Vignault J. C., Vogel R., McCoy R., Whitcomb R., Williamson D., Bové J. M. 1980; Caractérisation du spiroplasma isoles d’insectes et de fleurs de France continentale, de Corse et du Maroc: proposition pour une classification. C.R. Acad. Sci. Ser. D 290:1209–1211
     [Google Scholar]
  18. Konai M., Clark E. A., Whitcomb R. F. 1992 Temperature ranges and optima of spiroplasmas. 137 In Proceedings of the 9th International Congress of the International Organization for MycoplasmologyAmes, Iowa2 to 7 August 1992
     [Google Scholar]
  19. Le Goff F., Humphrey-Smith I., Leclercq M., Chastel C. 1991; Spiroplasmas from European Tabanidae. Med. Vet. Entomol. 5:143û144
     [Google Scholar]
  20. Rodwell A. W., Whitcomb R. F. 1983; Methods for direct and indirect measurement of mycoplasma growth. Methods Mycoplasmol. 1:185–196
     [Google Scholar]
  21. Shaikh A. A., Johnson W. E. Jr., Stevens C., Tang A. Y. 1987; The isolation of spiroplasmas from mosquitoes in Macon County, Alabama. J. Am. Mosq. Control Assoc. 3:289–295
     [Google Scholar]
  22. Tully J. G., Rose D. L., Clark E., Carle P., Bové J. M., Henegar R. B., Whitcomb R. F., Colflesh D. E., Williamson D. L. 1987; Revised group classification of the genus Spiroplasma (class Mollicutes), with proposed new groups XII to XXIII. Int. J. Syst. Bacteriol. 37:357–364
     [Google Scholar]
  23. Whitcomb R. F. 1983; Culture media for spiroplasmas. Methods Mycoplasmol. 1:147–158
     [Google Scholar]
  24. Whitcomb R. F., Bové J. M., Chen T. A., Tully J. G., Williamson D. L. 1987; Proposed criteria for an interim serogroup classification for members of the genus Spiroplasma (class Mollicutes). Int. J. Syst. Bacteriol. 37:82–84
     [Google Scholar]
  25. Whitcomb R. F., French F. E. Unpublished data
     [Google Scholar]
  26. Whitcomb R. F., Williamson D. L., Bové J. M., Tully J. G., Hackett K. J., Gasparich G. E., Abalain-Colloc M.-L. Unpublished data
     [Google Scholar]
  27. Williamson D. L., Tully J. G., Whitcomb R. F. 1979; Serological relationships of spiroplasmas as shown by combined deformation and metabolism inhibition tests. Int. J. Syst. Bacteriol. 29:345–351
     [Google Scholar]
  28. Williamson D. L., Tully J. G., Whitcomb R. F. 1989; The genus Spiroplasma. 71–109 In Tully J. G., Whitcomb R. F. (ed.) The mycoplasmas Academic Press, Inc.; New York:
     [Google Scholar]
  29. Williamson D. L., Whitcomb R. F. 1983; Special serological tests for spiroplasma identification. Methods Mycoplasmol. 2:249–259
     [Google Scholar]
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Serologic and Genomic Relatedness of Group VIII and Group XVII Spiroplasmas and Subdivision of Spiroplasma Group VIII into Subgroups
Int J Syst Evol Microbiol 43, 338 (1993); https://doi.org/10.1099/00207713-43-2-338
/content/journal/ijsem/10.1099/00207713-43-2-338
/content/journal/ijsem/10.1099/00207713-43-2-338
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