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Volume 43, Issue 4

Revised Taxonomy of the Methanotrophs: Description of gen. nov., Emendation of , Validation of and Species, and a Proposal that the Family Includes Only the Group I Methanotrophs Free

Abstract

Numerical taxonomic, DNA-DNA hybridization, and phospholipid fatty acid composition analyses were performed on an extensive range of methanotrophic strains, including reference strains and environmental isolates obtained from sites throughout eastern Australia. When the results of these studies were related to the results of a study based on genomic physicochemical properties, they clarified group I and II methanotroph genus and species interrelationships. The group I methanotrophs were found to be made up of three broadly phenotypically and genotypically homologous clusters of species. The first group I methanotroph cluster included the carotenoid-containing species , and These species represent the true members of the genus The second group I methanotroph cluster was made up of two subclusters of strains. One subcluster included species not capable of producing resting cells and consisted of the species “,” and The other subcluster included species capable of forming desiccation-resistant cysts and included , marine -like strains, and Strains designated “” and were found to be synonyms of , while was a synonym of It is proposed that these subclusters represent a new genus, gen. nov. The species in the new genus are type species comb. nov., sp. nov., sp. nov., nom. rev., sp. nov., comb. nov., and comb. nov. The remaining group I methanotrophs included the moderately thermophilic species and and a group of unnamed strains closely related to It is proposed that these species represent the true members of the genus The group II methanotrophs consisted of two closely related groups. The first group included budding, exospore-producing strains, while the second group included nonmotile, cyst-forming strains. These groups represent the genera and , which are revived here. The genus gen. nov., nom. rev. includes the species sp. nov., nom. rev. and sp. nov., nom. rev., while the genus gen. nov., nom. rev. includes the species sp. nov., nom. rev. and sp. nov., nom. rev.

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1993-10-01
2024-04-25
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Revised Taxonomy of the Methanotrophs: Description of Methylobacter gen. nov., Emendation of Methylococcus, Validation of Methylosinus and Methylocystis Species, and a Proposal that the Family Methylococcaceae Includes Only the Group I Methanotrophs
Int J Syst Evol Microbiol 43, 735 (1993); https://doi.org/10.1099/00207713-43-4-735
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