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Volume 44, Issue 1

sp. nov., an Anaerobic and Thermotolerant Facultative Alkaliphile Free

Abstract

An anaerobic and thermophilic alkaliphile, strain JW/YL23-2 (T = type strain), was isolated from sewage sludge obtained from a sewage plant in Atlanta, Ga. At pH 10.1 and 50°C, the doubling time of this strain was 19 min. Strain JW/YL23-2, a motile rod-shaped bacterium with 2 to 12 peritrichous flagella, exhibited a negative Gram stain reaction but was gram-type positive as judged by the polymyxin B test. No heat-stable (85°C, 15 min) endospores were detected. At 50°C, growth occurred at pH values ranging from 7.0 to 11.0; the optimum pH was 9.6 to 10.1. The temperature range for growth ranged from 27 to 57.5°C; the optimum temperature was 48 to 51°C (pH 10.1). Dissimilatory sulfate reduction was not detected. The organism utilized glucose, fructose, sucrose, maltose, cellobiose, and Casamino Acids. The DNA G+C content was 32 mol% (as determined by chemical analysis). A16S rRNA sequence analysis revealed a 2% inferred evolutionary distance to . However, the cell wall type of strain JW/YL23-2 was A4β (-Orn--Asp), while that of was (-diaminopimelic acid direct). On the basis of the alkaline pH values and high temperatures for optimal growth, the inability to form spores, and other characteristics different from C. characteristics, strain JW/YL-23-2 was placed in a new species, ; JW/YL23-2 (= DSM 7309) is the type strain of this new species.

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Copyright 1994, International Union of Microbiological Societies
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1994-01-01
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Clostridium thermoalcaliphilum sp. nov., an Anaerobic and Thermotolerant Facultative Alkaliphile
Int J Syst Evol Microbiol 44, 111 (1994); https://doi.org/10.1099/00207713-44-1-111
/content/journal/ijsem/10.1099/00207713-44-1-111
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