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Volume 44, Issue 1

Classification of by Restriction Endonuclease Analysis of Chromosomal DNA Free

Abstract

A total of 14 field strains originally labeled , 4 field strains of , and 12 type strains, as well as four additional reference strains, were classified by performing a restriction endonuclease analysis of chromosomal DNAs digested with 718, I, and RI. The patterns were analyzed by using (i) Jaccard coefficients and the unweighted pair group algorithm with arithmetic averages and (ii) principal-component analysis (PCA) and soft independent modeling of class analogy (SIMCA). Grouping by using the Jaccard coefficients and the unweighted pair group algorithm with arithmetic averages resulted in six clusters, defined at a similarity level of 69%. Cluster 1 comprised 10 field strains of sp. strain DSM 20056, and DSM 20016 (T = type strain) and could be divided into four subclusters. Cluster 2 included two field strains. Cluster 3 included one field strain labeled and one field strain labeled . Cluster 4 could be divided into three subclusters and included two field strains labeled , one reference strain and the type strain of , and four additional type strains. Cluster 5 contained two strains. Cluster 6 included two type strains. The two numerical methods gave the same general results, but the PCA-SIMCA method resulted in a more complete view of the relationships. The SIMCA analysis grouped DSM 20016, strain DSM 20056, and, with one exception, all of the field strains as a “class.” DSM 20075 and subsp. DSM 20008 were the type strains most closely related to the SIMCA class. The strains isolated from rats could be separated from the strains isolated from humans and pigs by the PCA. DNA-DNA-hybridization showed that strains classified as on the basis of the results of the restriction enzyme analysis also exhibited >70% DNA-DNA-homology to the type strain of . The phenotype of (ability to ferment carbohydrates) coincided in most cases with the genotype; this seemed not to be the case for strains.

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Copyright © 1994 International Union of Microbiological Societies
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1994-01-01
2024-04-19
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References

  1. Ahrné S. Personal communication
    [Google Scholar]
  2. Ahrné S., Molin G. 1991; Spontaneous mutations changing the raffinose metabolism of Lactobacillus plantarum. Antonie van Leeuweenhoek 60 87 93
    [Google Scholar]
  3. Axelsson L. 1990 Lactobacillus reuteri, a member of the gut bacterial flora Ph.D. thesis. Swedish University of Agricultural Sciences Department of Microbiology Report 44.; SLU/Repo, Uppsala, Sweden:
    [Google Scholar]
  4. Axelsson L., Lindgren S. 1987; Characterization and DNA homology of Lactobacillus strains isolated from pig intestine. J. Appl. Bacteriol. 62 433 440
    [Google Scholar]
  5. Axelsson L. T., Chung T. C., Dobrogosz W. J., Lindgren S. 1989; Production of broad spectrum antimicrobial substance by Lactobacillus reuteri. Microb. Ecol. Health Dis. 2 131 136
    [Google Scholar]
  6. Borch E., Molin G. 1988; Numerical taxonomy of psychroctrophic lactic acid bacteria from prepacked meat and meat products. Antonie van Leeuwenhoek 54 301 323
    [Google Scholar]
  7. Chassy B. M., Gibson E. M., Giuffrida A. 1976; Evidence for extrachromosomal elements in Lactobacillus. J. Bacteriol. 127 1576 1578
    [Google Scholar]
  8. Chung T. C., Axelsson L., Lindgren S. E., Dobrogosz W. J. 1989; In vitro studies on reuterin synthesis by Lactobacillus reuteri. Microb. Ecol. Health Dis. 2 137 144
    [Google Scholar]
  9. Collins M. D., Rodrigues U., Ash C., Aguirre M., Farrow J. A. E., Martinez-Murcia A., Phillips B. A., Williams A. M., Wallbanks S. 1991; Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA. FEMS Microbiol. Lett. 7 5 12
    [Google Scholar]
  10. Efthymiou C., Hansen P. A. 1962; An antigenic analysis of Lactobacillus acidophilus. J. Infect. Dis. 110 258 267
    [Google Scholar]
  11. Fabia R., Ar’Rajab A., Johansson M.-L., Willén R., Andersson R., Molin G., Bengmark S. 1993; The effect of exogenous administration of Lactobacillus reuteri R2LC and oat fiber on acetic acid-induced colitis in the rat. Scand. J. Gastroenterol. 28 155 162
    [Google Scholar]
  12. Kandier O., Stetter K.-O., Köhl R. 1980; Lactobacillus reuteri sp. nov., a new species of heterofermentative lactobacilli. Zentralbl. Bakteriol. Microbiol. Hyg. Abt. 1 Orig. Reihe C 1 264 269
    [Google Scholar]
  13. Kandier O., Weiss N. 1986; Regular, non-sporing Grampositive rods. 1208 1234 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Bergey’s manual of systematic bacteriology 2 Williams & Wilkins; Baltimore:
    [Google Scholar]
  14. Molin G., Jeppsson B., Ahrné S., Johansson M.-L., Nobaek S., Ståhl M., Bengmark S. 1993; Numerical taxonomy of Lactobacillus spp. associated to healthy and diseased mucosa of the human intestines. J. Appl. Bacteriol. 74 314 323
    [Google Scholar]
  15. Molin G., Johansson M.-L., Ståhl M., Ahrné S., Andersson R., Jeppsson B., Bengmark S. 1992; Systematics of the Lactobacillus population on rat intestinal mucosa with special reference to Lactobacillus reuteri. Antonie van Leeuwenhoek 61 175 183
    [Google Scholar]
  16. Romersburg H. C. 1984 Cluster analysis for research Lifetime Learning Publications; Belmont, Calif:
    [Google Scholar]
  17. Sneath P. H. A. 1978; Classification of microorganisms. 9/1 9/13 Norris J. R., Richmond M. H. Essays in microbiology Wiley; London:
    [Google Scholar]
  18. Ståhl M., Molin G., Persson A., Ahrné S., Ståhl S. 1990; Restriction endonuclease patterns and multi-variate analysis as a classification tool for Lactobacillus spp. Int. J. Syst. Bacteriol. 40 189 193
    [Google Scholar]
  19. Vescovo M., Dellaglio F., Bottazzi V., Sarra P. G. 1979; Deoxyribonucleic acid homology among Lactobacillus species of the subgenus Betabacterium Orla-Jensen. Microbiologica 2 317 330
    [Google Scholar]
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Classification of Lactobacillus reuteri by Restriction Endonuclease Analysis of Chromosomal DNA
Int J Syst Evol Microbiol 44, 9 (1994); https://doi.org/10.1099/00207713-44-1-9
/content/journal/ijsem/10.1099/00207713-44-1-9
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