1887

Abstract

We cloned the 5S rRNA gene and determined its organization in the four genes encoding rRNAs in a ribosomal DNA repeat unit of , the teleomorph of . The 5S rRNA gene contained 118 nucleotides and was located 1 kb upstream from the 18S rRNA gene within the 8.6-kb fragment of the ribosomal DNA repeat unit. The sequence of the 5S rRNA gene from was more similar to the sequence of the 5S rRNA gene from than to the sequences of the 5S rRNA genes from species. The arrangement of the rRNA genes in closely resembles the arrangement of the rRNA genes in mushrooms such as , and in that the 5S rRNA-coding region not only is located within the repeat unit that encodes the other rRNAs but also is transcribed in the same direction as the other rRNA genes. This is the first description of the arrangement of rRNA genes in a species belonging to the Heterobasidiomycetes.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-44-2-209
1994-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/44/2/ijs-44-2-209.html?itemId=/content/journal/ijsem/10.1099/00207713-44-2-209&mimeType=html&fmt=ahah

References

  1. Blanz P. A., Gottschalk M. 1984; A comparison of 5S ribosomal RNA nucleotide sequences from smut fungi. Syst. Appl. Microbiol. 5518–526
    [Google Scholar]
  2. Blanz P. A., Gottschalk M. 1986; Systematic position of Septobasidium, Graphiola and other Basidiomycetes as deduced on the basis of their 5S ribosomal RNA nucleotide sequences. Syst. Appl. Microbiol. 8121–127
    [Google Scholar]
  3. Blanz P. A., Unseld M. 1987; Ribosomal RNA as a taxonomic tool in mycology. Stud. Mycol. 30247–258
    [Google Scholar]
  4. Boekhout T., Fonseca A., Sampaio J. P., Golubev W. I. 1993; Classification of heterobasidiomycetous yeasts: characteristics and affiliation of genera to higher taxa of Heterobasidiomycetes. Can. J. Microbiol. 39276–290
    [Google Scholar]
  5. Borsuk P. A., Nagiec M. M., Stepien P. P., Bartnik E. 1982; Organization of the ribosomal RNA gene cluster in Aspergillus nidulans. Gene 17147–152
    [Google Scholar]
  6. Buckner B., Novotny C. P., Ullrich R. C. 1988; Organization of the ribosomal RNA genes of Schizophyllum commune. Curr. Genet. 13417–424
    [Google Scholar]
  7. Casadevall A. 1993 Personal communication
    [Google Scholar]
  8. Cassidy J. R., Moor D., Lu B. C., Pukkila P. J. 1984; Unusual organization and lack of recombination in the ribosomal RNA genes of Coprinus cinereus. Curr. Genet. 8607–613
    [Google Scholar]
  9. Cassidy J. R., Pukkila P. J. 1987; Inversion of 5S ribosomal RNA genes within the genus Coprinus. Curr. Genet. 1233–36
    [Google Scholar]
  10. Cihlar R. L., Syphered P. S. 1980; The organization of the ribosomal RNA genes in the fungus Mucor racemosus. Nucleic Acids Res. 8793–804
    [Google Scholar]
  11. Duchesne L. C., Anderson J. B. 1990; Location and direction of transcription of the 5S rRNA gene in Armillaria. Mycol. Res. 94266–269
    [Google Scholar]
  12. Free S. J., Rice P. W., Metzenberg R. L. 1979; Arrangement of the genes coding for ribosomal ribonucleic acids in Neurospora crassa. J. Bacteriol. 1371219–1226
    [Google Scholar]
  13. Garber R. C., Turgeon B. G., Selker E. U., Yoder O. C. 1988; Organization of ribosomal RNA genes in the fungus Cochliobolus heterostrophus. Curr. Genet. 14573–582
    [Google Scholar]
  14. Gottschalk M., Blanz P. A. 1984; Highly conserved 5S ribosomal RNA sequences in four rust fungi and atypical 5S rRNA secondary structure in Microstroma juglandis. Nucleic Acids Res. 123951–3958
    [Google Scholar]
  15. Howlett B. J., Brownlee A. G., Guest D. I., Adcock G. J., McFadden G. I. 1992; The 5S ribosomal RNA gene is linked to large and small subunit ribosomal rRNA genes in oomycetes, Phytophthora vignae, P. cinnamomi, P. megasperma f. sp. glycinea and Saprolegnia ferax. Curr. Genet. 22455–461
    [Google Scholar]
  16. Kwon-Chung K. J. 1987; Filobasidiaceae—a taxonomic survey. Stud. Mycol. 3075–85
    [Google Scholar]
  17. Long E. O., David I. B. 1980; Repeated genes in eukaryotes. Annu. Rev. Biochem. 49727–764
    [Google Scholar]
  18. Mitchell T. G., White T. J., Taylor J. W. 1992; Comparison of 5.8S ribosomal DNA sequences among the basidiomycetous yeast genera Cystofilobasidium, Filobasidium and Filobasidiella. J. Med. Vet. Mycol. 30207–218
    [Google Scholar]
  19. Nath K., Bollon A. P. 1977; Organization of the yeast ribosomal RNA gene cluster via cloning and restriction analysis. J. Biol. Chem. 2526562–6571
    [Google Scholar]
  20. Perry R. P. 1976; Processing of RNA. Annu. Rev. Biochem. 45605–629
    [Google Scholar]
  21. Restrepo B. I., Barbour A. G. 1989; Cloning of 18S and 25S rDNAs from the pathogenic fungus Cryptococcus neoformans. J. Bacteriol. 1715596–5600
    [Google Scholar]
  22. Rozek C. E., Timberlake W. E. 1979; Restriction endonuclease mapping by crossed contact hybridization: the ribosomal RNA genes of Achlya ambisexualis. Nucleic Acids Res. 81567–1578
    [Google Scholar]
  23. Selker E. U., Yanofsky C., Driftmier K., Metzenberg R. L., Alzner-DeWeerd B., Raj Bhandary U. L. 1981; Dispersed 5S RNA genes in N. crassa: structure, expression and evolution. Cell 24819–828
    [Google Scholar]
  24. Tabata S. 1980; Structure of the 5-S ribosomal RNA gene and its adjacent regions in Torulopsis utilis. Eur. J. Biochem. 110107–114
    [Google Scholar]
  25. Tabata S. 1981; Nucleotide sequences of the 5S ribosomal RNA genes and their adjacent regions in Schizosaccharomyces pombe. Nucleic Acids Res. 96429–6437
    [Google Scholar]
  26. Valenzuela P., Bell G. I., Venegas A., Swell E. L., Masiarz F. R., Digennaro L. J., Weinberg J., Rutter W. J. 1977; Ribosomal RNA gene of Saccharomyces cerevisiae. J. Biol. Chem. 2528126–8135
    [Google Scholar]
  27. Van Heerikhuizen H., Ykema A., Klootwijk J., Gaillardin C., Balias C., Fournier P. 1985; Heterogeneity in the ribosomal RNA genes of the yeast Yarrowia lipolytica: cloning and analysis of two size classes of repeats. Gene 39213–222
    [Google Scholar]
  28. Varma A., Kwon-Chung K. J. 1991; A rapid method to extract DNA from Cryptococcus neoformans. J. Clin. Microbiol. 29810–812
    [Google Scholar]
  29. Varma A., Kwon-Chung K. J. 1992; A DNA probe for strain typing in Cryptococcus neoformans. J. Clin. Microbiol. 302960–2967
    [Google Scholar]
  30. Verbeet M. P., Klootwijk J., van Heerikhuizen H., Fontijn R., Vreugdenhil E., Planta R. J. 1983; Molecular cloning of the rDNA of Saccharomyces rosei and comparison of its transcription initiation region with that of Saccharomyces carlsbergensis. Gene 2353–63
    [Google Scholar]
  31. Verbeet M. P., van Heerikhuizen H., Klootwijk J., Fontijn R. D., Planta R. J. 1984; Evolution of yeast ribosomal DNA: molecular cloning of the rDNA units of Kluyveromyces lactis and Hansenula wingei and their comparison with the rDNA units of other Saccharomycetoideae. Mol. Gen. Genet. 195116–125
    [Google Scholar]
  32. Vilgalys R., Gonzales D. 1990; Organization of ribosomal DNA in the basidiomycete Thanatephorus praticola. Curr. Genet. 18277–280
    [Google Scholar]
  33. Walker W. F. 1984; 5S rRNA sequences from Atractiellales, and basidiomycetous yeasts and fungi imperfecti. Syst. Appl. Microbiol. 5448–456
    [Google Scholar]
  34. Walker W. F., Doolittle W. F. 1982; Redividing the basidiomycetes on the basis of 5S rRNA sequences. Nature (London) 299723–724
    [Google Scholar]
  35. Walker W. F., Doolittle W. F. 1983; 5S rRNA sequences from eight basidiomycetes and fungi imperfecti. Nucleic Acids Res. 117625–7630
    [Google Scholar]
  36. Weinmann R., Roeder R. G. 1974; Role of DNA-dependent RNA polymerase III in the transcription of tRNA and 5S RNA genes. Proc. Natl. Acad. Sci. USA 711790–1794
    [Google Scholar]
  37. Wiekes B. L., Moor T. D. E., Kwon-Chung K. J. Comparison of the electrophoretic karyotypes and chromosomal location of ten genes in the two varieties of Cryptococcus neoformans. Microbiology in press
    [Google Scholar]
  38. Wolters J., Erdmann U. A. 1988; Compilation of 5S rRNA gene sequences. Nucleic Acids Res. 16Srl–r70
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-44-2-209
Loading
/content/journal/ijsem/10.1099/00207713-44-2-209
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error