1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 44, Issue 3

sp. nov., a Moderately Halophilic, Anaerobic Bacterium from a Subterranean Brine Free

Abstract

Abstract

A strictly anaerobic, moderately halophilic, gram-negative bacterium was isolated from a highly saline oil field brine. The bacterium was a non-spore-forming, nonmotile rod, appearing singly, in pairs, or occasionally as long chains, and measured 0.3 to 0.4 by 2.6 to 4 μm. The bacterium had a specific requirement for NaCl and grew at NaCl concentrations of between 6 and 24%, with optimal growth at 9% NaCl. The isolate grew at temperatures of between 22 and 51°C and pH values of between 5.6 and 8.0. The doubling time in a complex medium containing 10% NaCl was 9 h. Growth was inhibited by chloramphenicol, tetracycline, and penicillin but not by cycloheximide or azide. Fermentable substrates were predominantly carbohydrates. The end products of glucose fermentation were acetate, ethanol, CO, and H. The major components of the cellular fatty acids were C, C, C, and C acids. The DNA base composition of the isolate was 34 mol% G+C. Oligonucleotide catalog and sequence analyses of the 16S rRNA showed that strain VS-752 was most closely related to GSL (ATCC 33744), the sole member of the genus We propose that strain VS-752 (ATCC 51327) be established as the type strain of a new species, , in the genus .

  • Published Online:
Copyright 1994 International Union of Microbiological Societies
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-44-3-565
1994-01-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/44/3/ijs-44-3-565.html?itemId=/content/journal/ijsem/10.1099/00207713-44-3-565&mimeType=html&fmt=ahah

References

  1. Balch W. E., Wolfe R. S. 1976; New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl. Environ. Microbiol. 32:781–791
     [Google Scholar]
  2. Belyaev S. S., Charackhchian I. A., Borzenkov I. A., Milyokhina E. I., Kuzetsova V. G. 1990 The activities of microbiological processes in stratal waters of oil fields. 425–443 In Fliermans C.B., Hazen T.C. (ed.) Proceedings of the 1st International Symposium on Microbiology of the Deep SubsurfaceWSRC Information ServicesAiken, S.C.
     [Google Scholar]
  3. Bhupathiraju V. K., Mclnerney M. J., Knapp R. M. 1993; Pretest studies for a microbially enhanced oil recovery field pilot in a hypersaline oil reservoir. Geomicrobiol. J. 11:19–34
     [Google Scholar]
  4. Bhupathiraju V. K., Sharma P. K., Mclnerney M. J., Knapp R. M., Fowler K., Jenkins W. 1991; Isolation and characterization of novel halophilic anaerobic bacteria from oil field brines. Dev. Petrol. Sci. 31:131–143
     [Google Scholar]
  5. Cato E.P., Stackebrandt E. 1989 Taxonomy and phylogeny1–26 In Minton N.P., Clarke D.J. (ed.) Clostridia. Plenum Press; New York:
     [Google Scholar]
  6. De Soete G. 1983; A least square algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626
     [Google Scholar]
  7. De Weerd K. A., Mandelco L., Tanner R. S., Woese C. R., Suflita J. M. 1990; Desulfomonile tiedjei gen. nov. and sp. nov., a novel anaerobic, dehalogenating, sulfate-reducing bacterium. Arch. Microbiol. 154:23–30
     [Google Scholar]
  8. Dussault H. P. 1955; An improved technique for staining red halophilic bacteria. J. Bacteriol. 70:484–485
     [Google Scholar]
  9. Gervertz D., Paterek J. R., Davey M. E., Wood W. A. 1991; Isolation and characterization of anaerobic halophilic bacteria from oil reservoir brines. Dev. Petrol. Sci. 31:115–129
     [Google Scholar]
  10. Jenneman G. E., Mclnerney M. J., Knapp R. M. 1986; Effect of nitrate on biogenic sulfide production. Appl. Environ. Microbiol. 51:1205–1211
     [Google Scholar]
  11. Johnson J. L., Francis B. S. 1975; Taxonomy of the clostridia: ribosomal ribonucleic acid homologies among species. J. Gen. Microbiol. 88:229–244
     [Google Scholar]
  12. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. 21–132 In Monro H.N. (ed.) Mammalian protein metabolism vol. 3 Academic Press; New York:
     [Google Scholar]
  13. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analysis. Proc. Natl. Acad. Sci. USA 82:6955–6959
     [Google Scholar]
  14. Liaw H. J., Mah R. A. 1992; Isolation and characterization of Haloanaerobacter chitinovorans gen. nov., sp. nov., a halophilic, anaerobic, chitinolytic bacterium from a solar saltern. Appl. Environ. Microbiol. 58:260–266
     [Google Scholar]
  15. Madigan M. T. 1992; The family Heliobacteriaceae. 1981–1992 In Balows A., Trüper H.G., Dworkin M., Harder W., Schleifer K.H. (ed.) The prokaryotes, 2nd ed.. vol. 2 Springer-Verlag; New York:
     [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208–218
     [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39:159–167
     [Google Scholar]
  18. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J. Clin. Microbiol. 16:584–586
     [Google Scholar]
  19. Moss C. W., Wallace P. L., Hollis D. G., Weaver R. E. 1988; Cultural and chemical characterization of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis. J. Clin. Microbiol. 26:484–492
     [Google Scholar]
  20. Ni S., Boone D. R. 1991; Isolation and characterization of a dimethyl sulfide-degrading methanogen, Methanolobus siciliae HI350, from an oil well, characterization of M. siciliae T4/MT, and emendation of M. siciliae. Int. J. Syst. Bacteriol. 41:410–416
     [Google Scholar]
  21. Oren A. 1983; Clostridium lortetii sp. nov. a halophilic obligatorily anaerobic bacterium producing endospores with attached gas vacuoles. Arch. Microbiol. 136:42–48
     [Google Scholar]
  22. Oren A, Paster B. J., Woese C. R. 1984; Haloanaerobiaceae: a new family of moderately halophilic, obligatory anaerobic bacteria. Syst. Appl. Microbiol. 5:71–80
     [Google Scholar]
  23. Oren A, Pohla H., Stackebrandt E. 1987; Transfer of Clostridium lortetii to a new genus Sporohalobacter gen. nov. as Sporohalobacter lortetii comb, nov., and description of Sporohalobacter marismortui sp. nov.. Syst. Appl. Microbiol. 9:239–246
     [Google Scholar]
  24. Oren A., Weisburg W. G., Kessel M., Woese C. R. 1984; Halobacteroides halobius gen. nov., sp. nov., a moderately halophilic anaerobic bacterium from the bottom sediments of the Dead Sea. Syst. Appl. Microbiol. 5:58–70
     [Google Scholar]
  25. Oyaizu H., Debrunner-Vossbrinck B., Mandelco L., Studier J. A., Woese C. R. 1987; The green non-sulfur bacteria: a deep branching in the eubacterial line of descent. Syst. Appl. Microbiol. 9:47–53
     [Google Scholar]
  26. Rainey F. A., Stackebrandt E. 1993; Phylogenetic analysis of the bacterial genus Thermobacteroides indicates an ancient origin of Thermobacteroides proteolyticus. Lett. Appl. Microbiol. 16:282–286
     [Google Scholar]
  27. Rengpipat S., Langworthy T. A., Zeikus J. G. 1988; Halobacteroides acetoethylicus sp. nov., a new obligately anaerobic halophile isolated from deep subsurface hypersaline environments. Syst. Appl. Microbiol. 11:28–35
     [Google Scholar]
  28. Sassar M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101 MIDI, Inc.; Newark, Del.:
     [Google Scholar]
  29. Shiba H. 1991; Anaerobic halophiles. 191–211 In Horikoshi K., Grant W. D. (ed.) Superbugs, microorganisms in extreme environments Japan Scientific Societies Press; Tokyo:
     [Google Scholar]
  30. Shiba H., Yamamoto H., Horikoshi K. 1989; Isolation of strictly anaerobic halophiles from the aerobic surface sediments of hypersaline environments in California and Nevada. FEMS Microbiol. Lett. 57:191–196
     [Google Scholar]
  31. Smibert R. M., Krieg N. R. 1981; General characterization. 409–443 In Gerhardt P., Murray R. G. E., Costilow R.N., Nester E.W., Wood W.A., Krieg N.R., Phillips G.B. (ed.) Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
     [Google Scholar]
  32. Tanner R. S. 1989; Monitoring sulfate-reducing bacteria: comparison of enumeration media. J. Microbiol. Methods 10:83–90
     [Google Scholar]
  33. Tanner R. S., Miller L. M., Yang D. 1993; Clostridium ljungdahlii sp. nov., an acetogenic species in clostridial rRNA homology group I. Int. J. Syst. Bacteriol. 43:232–236
     [Google Scholar]
  34. Ventosa A. 1988; Taxonomy of moderately halophilic heterotrophic eubacteria. 71–84 In Rodriguez-Valera F. (ed.) Halophilic bacteria vol. 1 CRC Press, Inc.; Boca Raton, Fla:
     [Google Scholar]
  35. Vreeland R. H., Hochstein L. I. (ed.) 1992 The biology of halophilic bacteria CRC Press, Inc.; Boca Raton, Fla:
     [Google Scholar]
  36. Zeikus J. G., Hegge P. W., Thompson T. E., Phelps T. J., Langworthy T. A. 1983; Isolation and description of Haloanaerobium praevalens gen. nov. sp. nov., an obligately anaerobic halophile common to Great Salt Lake sediments. Curr. Microbiol. 9:225–234
     [Google Scholar]
  37. Zhao H., Yang D., Woese C. R., Bryant M. P. 1993; Assignment of fatty acid-β-oxidizing syntrophic bacteria to Syntrophomonadaceae fam. nov. on the basis of 16S rRNA sequence analyses. Int. J. Syst. Bacteriol. 43:278–286
     [Google Scholar]
  38. Zhilina T. N., Kevbrin V. V., Lysenko A. M., Zavarzin G. A. 1991; Saccharolytic anaerobic bacteria in a halophilic cyariobacterial mat. Microbiology 60:101–107
     [Google Scholar]
  39. Zhilina T. N., Miroshinikova L. V., Osipov G. A., Zavarzin G. A. 1991; Halobacteroides lacunaris sp. nov., a new saccharolytic anaerobic extremely halophilic bacterium from a hypersaline Chokrack Lake. Microbiology 60:495–503
     [Google Scholar]
  40. Zhilina T. N., Zavarzin G. A., Bulygina E. S., Kevbrin V. V., Ósipov G. A., Chumakov K. M. 1992; Ecology, physiology and taxonomy studies on a new taxon of Haloanaerobiaceae, Haloincola saccharolytica gen. nov., sp. nov.. Syst. Appl. Microbiol. 15:275–284
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-44-3-565
Loading
Haloanaerobium salsugo sp. nov., a Moderately Halophilic, Anaerobic Bacterium from a Subterranean Brine
Int J Syst Evol Microbiol 44, 565 (1994); https://doi.org/10.1099/00207713-44-3-565
/content/journal/ijsem/10.1099/00207713-44-3-565
/content/journal/ijsem/10.1099/00207713-44-3-565
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error