%0 Journal Article %A Guillamón, José Manuel %A Barrio, Eladio %A Huerta, Tomás %A Querol, Amparo %T Rapid Characterization of Four Species of the Saccharomyces Sensu Stricto Complex According to Mitochondrial DNA Patterns %D 1994 %J International Journal of Systematic and Evolutionary Microbiology, %V 44 %N 4 %P 708-714 %@ 1466-5034 %R https://doi.org/10.1099/00207713-44-4-708 %I Microbiology Society, %X Abstract Several strains of the four sibling species of the genus Saccharomyces (S. bayanus, S. cerevisiae, S. paradoxus, and S. pastorianus) were characterized by using a rapid and simple method of restriction analysis of mitochondrial DNA. Patterns obtained with four-cutter endonucleases (such as AluI, DdeI, HinfI, and RsaI) made it possible to differentiate each species. 5. cerevisiae and S. paradoxus presented a greater number of large fragments than S. pastorianus and S. bayanus with all the assay enzymes. With AluI and DdeI, species-specific bands clearly permitted differentiation between S. pastorianus and S. bayanus. To test the resolution of this method, wild Saccharomyces strains were analyzed. The correct assignment of these strains to a known taxon by this rapid method was confirmed by means of electrophoretic karyotyping. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-44-4-708