RT Journal Article SR Electronic(1) A1 Guillamón, José Manuel A1 Barrio, Eladio A1 Huerta, Tomás A1 Querol, AmparoYR 1994 T1 Rapid Characterization of Four Species of the Saccharomyces Sensu Stricto Complex According to Mitochondrial DNA Patterns JF International Journal of Systematic and Evolutionary Microbiology, VO 44 IS 4 SP 708 OP 714 DO https://doi.org/10.1099/00207713-44-4-708 PB Microbiology Society, SN 1466-5034, AB Abstract Several strains of the four sibling species of the genus Saccharomyces (S. bayanus, S. cerevisiae, S. paradoxus, and S. pastorianus) were characterized by using a rapid and simple method of restriction analysis of mitochondrial DNA. Patterns obtained with four-cutter endonucleases (such as AluI, DdeI, HinfI, and RsaI) made it possible to differentiate each species. 5. cerevisiae and S. paradoxus presented a greater number of large fragments than S. pastorianus and S. bayanus with all the assay enzymes. With AluI and DdeI, species-specific bands clearly permitted differentiation between S. pastorianus and S. bayanus. To test the resolution of this method, wild Saccharomyces strains were analyzed. The correct assignment of these strains to a known taxon by this rapid method was confirmed by means of electrophoretic karyotyping., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-44-4-708