- International Journal of Systematic and Evolutionary Microbiology
- Volume 44, Issue 4
f Diversity of Borrelia burgdorfeii Sensu Lato Evidenced by Restriction Fragment Length Polymorphism of rrf (5S)-rrl (23S) Intergenic Spacer Amplicons
- Authors: DANIELE POSTIC*, MARC V. ASSOUS†, PATRICK A. D. GRIMONT, GUY BARANTON
- * Corresponding author. Mailing address: Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 83 37. Fax: 33 1 40 61 30 01. Electronic mail address: firstname.lastname@example.org.
- Int J Syst Evol Microbiol, October 1994 44: 743-752, doi: 10.1099/00207713-44-4-743
- Subject: Original Papers Relating To Systematic Bacteriology
- Published Online:
The organization of the ribosomal genes is unique in Borrelia burgdorferi in that the rrl (23S) and rrf (5S) genes are tandemly duplicated. We took advantage of this uniqueness to assess the restriction polymorphism of PCR products obtained with primers at the 3′ end of the first rrf gene and at the 5′ end of the second rrl gene. An amplicon that was 226 to 266 bp long was generated from 99 of 100 B. burgdorferi sensu lato strains. The nuclease MseI restriction polymorphism of the amplicons provided a useful tool for identifying B. burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii (formerly group VS461), and Borrelia japonica (formerly group F63B). Furthermore, it allowed us to recognize four new genomic groups, which were confirmed by DNA-DNA hybridization data. Two of these genomic groups comprised European strains, and the other two groups contained American strains. The American genomic groups involved vectors with enzootic cycles quite different from those of B. burgdorferi sensu stricto, which previously was the only Lyme disease Borrelia species known to occur in the United States. Our method could be used for rapid screening of strain collections and for epidemiological and medical purposes.
Present address: Faculté de Médecine Cochin-Port-Royal, 75674 Paris Cedex 14, France.
Copyright © 1994 International Union of Microbiological Societies | Published by the Microbiology Society
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