@article{mbs:/content/journal/ijsem/10.1099/00207713-45-1-116, author = "DYKSTERHOUSE, SHERYL E. and GRAY, JAMES P. and HERWIG, RUSSELL P. and LARA, J. CANO and STALEY, JAMES T.", title = "Cycloclasticus pugetii gen. nov., sp. nov., an Aromatic Hydrocarbon-Degrading Bacterium from Marine Sediments", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1995", volume = "45", number = "1", pages = "116-123", doi = "https://doi.org/10.1099/00207713-45-1-116", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-45-1-116", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Three heterotrophic bacterial strains were isolated from different locations in Puget Sound, Washington, by using biphenyl as the principal carbon source. These strains grow by using a limited number of organic compounds, including the aromatic hydrocarbons naphthalene, phenanthrene, anthracene, and toluene, as sole carbon sources. These aerobic, gram-negative rods are motile by means of single polar flagella. Their 16S rRNA sequences indicate that they are all members of the γ subdivision of the Proteobacteria. Their closest known relatives are the genera Methylobacter and Methylomonas (genera of methane-oxidizing bacteria), uncultured sulfur-oxidizing symbionts found in marine invertebrates, and clone FL5 containing 16S ribosomal DNA amplified from an environmental source. However, the Puget Sound bacteria do not use methane or methanol as a carbon source and do not oxidize reduced sulfur compounds. Furthermore, a 16S rRNA base similarity comparison revealed that these bacteria are sufficiently different from other bacteria to justify establishment of a new genus. On the basis of the information summarized above, we describe a new genus and species, Cycloclasticus pugetii, for these bacteria; strain PS-1 is the type strain of C. pugetii.", }