- International Journal of Systematic and Evolutionary Microbiology
- Volume 45, Issue 1
f Genomic Diversity and Phylogenetic Relationships among Lipid-Requiring Diphtheroids from Humans and Characterization of Corynebacterium macginleyi sp. nov.
- Authors: P. RIEGEL*, R. RUIMY, D. DE BRIEL, G. PRéVOST, F. JEHL, R. CHRISTEN, H. MONTEIL
- * Corresponding author. Mailing address: Institut de Bactériologie de la Faculté de Médecine, 3 rue Koeberlé, F-67000 Strasbourg, France. Phone: 220.127.116.11.70. Fax: 18.104.22.168.13.
- First Published Online: 01 January 1995, International Journal of Systematic and Evolutionary Microbiology 45: 128-133, doi: 10.1099/00207713-45-1-128
- Subject: Original Papers Relating To Systematic Bacteriology
- Issue Published:
DNA relatedness experiments were performed with 38 clinical isolates and 13 reference strains of coryneform taxa exhibiting a lipid requirement for optimal growth. Forty-five of these strains split into five genomic groups at the species level, whereas six other strains remained unclustered. Genomospecies II fits Corynebacterium accolens, but the other genomospecies were not genetically related to any of the defined Corynebacterium species. Phylogenetic analyses of genes coding for small-subunit rRNA sequences revealed that two genomospecies (I and III) and C. accolens form a tight cluster within the robust branch that groups all Corynebacterium species presently sequenced. Reference strains of biotypes C-1, C-2, and C-3 of “Corynebacterium pseudogenitalium” were found to fall into genomospecies I, as well as “Corynebacterium tuberculostearicum,” Centers for Disease Control and Prevention (CDC) coryneform group G-1, and CDC coryneform group G-2 reference strains. Biochemical tests allowed differentiation between genomospecies except between genomospecies IV and V and between six unclustered strains and genomospecies I. We propose a new classification for these lipid-requiring diphtheroids within the genus Corynebacterium with the delineation of some CDC coryneform group G-1 strains (genomospecies III) as a new species for which the name Corynebacterium macginleyi is proposed. The type strain is strain JCL-2 (CIP 104099), isolated from a human corneal ulcer.
Copyright © 1995 International Union of Microbiological Societies | Published by the Microbiology Society
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