1887

Abstract

Because of the very similar physiological properties and base sequences of the 16S rRNAs of , , and , some taxonomists question the validity of separating these organisms into distinct species. DNA relatedness studies based on spectrophotometrically measured renaturation rates were carried out to determine the taxonomic relationships of the three species. A study of 58 strains revealed that the levels of relatedness between . and . and between . and . ranged from 22 to 44% and from 59 to 69%, respectively. On the basis of the moderately high levels of DNA relatedness which we determined, . and . appeared to be genetically related but taxonomically distinct entities. The . group was genetically distantly related to the . group and represented a separate taxon. Furthermore, our data indicated that the . group consists of two genetically distinct groups, each of which represents a distinct species. In addition to rhizoidal colonial morphology and lack of motility, the . group could be distinguished from . by differences in fatty acid profiles and acetanilide-producing activities.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-45-1-46
1995-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/45/1/ijs-45-1-46.html?itemId=/content/journal/ijsem/10.1099/00207713-45-1-46&mimeType=html&fmt=ahah

References

  1. Ash C., Farrow J. A. E., Dorsch M., Stackebrandt E., Collins M. D. 1991; Comparative analysis of Bacillus anthracis Bacillus cereus and related species on the basis of reverse transcriptase sequencing of 16S rRNA. Int. J. Syst. Bacteriol 41:343–346
    [Google Scholar]
  2. Collins M. D., Williams A. M., Wallbanks S. 1990; The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov. FEMS Microbiol. Lett 70:255–262
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem 12:133–142
    [Google Scholar]
  4. Gordon R. E., Haynes W. C., Pang C. H.-N. 1973; The genus Bacillus. U. S. Dep. Agric. Agric. Handb 427:23–30116–119
    [Google Scholar]
  5. Haynes W. C., Wickerham L. J., Hesseltine C. W. 1955; Maintenance of cultures of industrially important microorganisms. Appl. Microbiol 3:361–368
    [Google Scholar]
  6. Kaneko T., Nozaki R., Aizawa K. 1978; Deoxyribonucleic acid related-ness between Bacillus anthracis Bacillus cereus and Bacillus thuringiensis. Microbiol. Immunol 22:639–641
    [Google Scholar]
  7. Maniatis T., Frisch E. F., Sambrook J. 1982; Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Cold Spring, N. Y.;
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  9. Nakamura L. K. 1994; DNA relatedness among Bacillus thuringiensis serovars. Int. J. Syst. Bacteriol 44:125–129
    [Google Scholar]
  10. Nakamura L. K., Swezey J. 1983; Taxonomy of Bacillus circulans Jordan 1890: base composition and reassociation of deoxyribonucleic acid. Int. J. Syst. Bacteriol 33:46–52
    [Google Scholar]
  11. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101 Microbial ID, Inc Newark: Del;
    [Google Scholar]
  12. Seki T., Chung C., Mikami H., Oshima Y. 1978; Deoxyribonucleic acid homology and taxonomy of the genus Bacillus. Int. J. Syst. Bacteriol 28:182–189
    [Google Scholar]
  13. Skerman V. B. D., McGowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol 30:225–420
    [Google Scholar]
  14. Smith N. R., Gordon R. E., Clark F. E. 1952; Aerobic sporeforming bacteria. Monograph no. 16 U. S. Department of Agriculture; Washington, D. C.:
    [Google Scholar]
  15. Sneath P. H. A., Sokal R. R. 1973 Numerical taxonomy W. H. Freeman and Co; San Francisco:
    [Google Scholar]
  16. Sommerville H. J., Jones M. L. 1972; DNA competition studies within the Bacillus cereus group of bacilli. J. Gen. Microbiol 73:257–265
    [Google Scholar]
  17. Williams A. M., Rodrigues U. M., Collins M. D. 1991; Intragenic relationships of Enterococcus as determined by reverse transcriptase sequencing of small-subunit rRNA. Res. Microbiol 142:67–74
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-45-1-46
Loading
/content/journal/ijsem/10.1099/00207713-45-1-46
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error