- International Journal of Systematic and Evolutionary Microbiology
- Volume 45, Issue 2
f Succiniclasticum ruminis gen. nov., sp. nov., a Ruminal Bacterium Converting Succinate to Propionate as the Sole Energy-Yielding Mechanism
- By N. O. van GYLSWYK
- First Published Online: 01 April 1995, International Journal of Systematic and Evolutionary Microbiology 45: 297-300, doi: 10.1099/00207713-45-2-297
- Subject: Original Papers Relating To Systematic Bacteriology
- Issue Published:
A gram-negative, anaerobic, nonmotile, non-spore-forming, rod-shaped bacterium that fermented succinate quantitatively to propionate was isolated from a high dilution of rumen ingesta obtained from a dairy cow fed a production diet containing grass silage as the main roughage source. This organism did not grow on any of the following energy sources: 12 carbohydrates, pyruvate, lactate, 7 dicarboxylic acids, aspartate, citrate, and trans-aconitate. Both rumen fluid and yeast extract were necessary for good growth on succinate. The organism was negative for the following characteristics: production of propionate from threonine, protein digestion, sulfide production, nitrate reduction, catalase activity, and urease activity. There was no growth at 22°C and reduced growth at 45°C compared with growth at 39°C. The DNA base composition was 52 mol% G+C. The complete 16S rRNA sequence (EMBL accession number, X81137) was obtained, and the phylogenetic relationships of the organism were determined. The most closely related genera were the genera Acidaminococcus and Phascolarctobacterium. The name proposed for this bacterium is Succiniclasticum ruminis gen. nov., sp. nov.; the type strain is strain SE10 (= DSM 9236). Additional isolation attempts revealed that S. ruminis is a common inhabitant of the rumina of cows that are fed production diets and of cows on pasture.
Copyright © 1995, International Union of Microbiological Societies | Published by the Microbiology Society
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