RT Journal Article SR Electronic(1) A1 ENGLE, MARCELLA A1 LI, YOUHONG A1 WOESE, CARL A1 WIEGEL, JUERGENYR 1995 T1 Isolation and Characterization of a Novel Alkalitolerant Thermophile, Anaerobranca horikoshii gen. nov., sp. nov. JF International Journal of Systematic and Evolutionary Microbiology, VO 45 IS 3 SP 454 OP 461 DO https://doi.org/10.1099/00207713-45-3-454 PB Microbiology Society, SN 1466-5034, AB Nine moderately alkalitolerant thermophilic bacteria with similar properties were isolated from water and soil samples obtained from Yellowstone National Park. These Gram-type-positive, rod-shaped bacteria produce cells with primary branches. The cells are peritrichous and exhibit only slight tumbling motility. At 60°C the pH range for growth is 6.9 to 10.3, and the optimum pH is 8.5. At pH 8.5 the temperature range for growth is 34 to 66°C, with an optimum temperature of 57°C. The strains are mainly proteolytic. The fermentation products from yeast extract are acetate, CO2, and H2. Fumarate added to minimal medium containing yeast extract is stoichiometrically converted to succinate, indicating that it is used as an alternative electron acceptor. The DNA G+C content is 33 to 34 mol%. On the basis of its unique properties, such as branch formation, growth at alkaline pH values at elevated temperatures, and the relative distance of its 16S rRNA sequence from those of other known bacteria, we propose that strain JW/YL-138T (T = type strain) and eight similar strains represent a new genus and species, Anaerobranca horikoshii. Strain JW/YL-138 is designated the type strain of the type species, A. horikoshii, which was named in honor of Koki Horikoshi, a pioneer in the field of alkaliphilic bacteria., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-45-3-454