- International Journal of Systematic and Evolutionary Microbiology
- Volume 45, Issue 3
f Partial Gene Sequences for the A Subunit of Methyl-Coenzyme M Reductase (mcrI) as a Phylogenetic Tool for the Family Methanosarcinaceae
- Authors: ERIK SPRINGER†, MATTHEW S. SACHS, CARL R. WOESE, DAVID R. BOONE†
- * Corresponding author. Mailing address: Department of Environmental Science and Engineering, Oregon Graduate Institute of Science & Technology, P.O. Box 91000, Portland, OR 97291-1000. Phon: (503) 690-1146. Fax: (503) 690-1273. Electronic mail addres: email@example.com.
- Int J Syst Evol Microbiol, July 1995 45: 554-559, doi: 10.1099/00207713-45-3-554
- Subject: Original Papers Relating To Systematic Bacteriology
- Published Online:
Representatives of the family Methanosarcinaceae were analyzed phylogenetically by comparing partial sequences of their methyl-coenzyme M reductase (mcrI) genes. A 490-bp fragment from the A subunit of the gene was selected, amplified by the PCR, cloned, and sequenced for each of 25 strains belonging to the Methanosarcinaceae. The sequences obtained were aligned with the corresponding portions of five previously published sequences, and all of the sequences were compared to determine phylogenetic distances by Fitch distance matrix methods. We prepared analogous trees based on 16S rRNA sequences; these trees corresponded closely to the mcrI trees, although the mcrI sequences of pairs of organisms had 3.01 ± 0.541 times more changes than the respective pairs of 16S rRNA sequences, suggesting that the mcrI fragment evolved about three times more rapidly than the 16S rRNA gene. The qualitative similarity of the mcrI and 16S rRNA trees suggests that transfer of genetic information between dissimilar organisms has not significantly affected these sequences, although we found inconsistencies between some mcrI distances that we measured and previously published DNA reassociation data. It is unlikely that multiple mcrI isogenes were present in the organisms that we examined, because we found no major discrepancies in multiple determinations of mcrI sequences from the same organism. Our primers for the PCR also match analogous sites in the previously published mcrII sequences, but all of the sequences that we obtained from members of the Methanosarcinaceae were more closely related to mcrI sequences than to mcrII sequences, suggesting that members of the Methanosarcinaceae do not have distinct mcrII genes.
Present address: Carl von Ossietzky Universität Oldenburg, AG Mikrobiologie, 26111 Oldenburg, Germany.
Copyright © 1995 International Union of Microbiological Societies | Published by the Microbiology Society
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