1887

Abstract

Analyses of the sequences of the small-subunit (18S) rRNA gene and two internal transcribed spacers (ITSs), ITS1 and ITS2, revealed that members of the yeast genera and are phylogenetically intermixed. Despite some minor differences in 18S rRNA-, ITS1-, and ITS2-derived trees, in general the patterns of the relationships inferred from the three chronometers were in good agreement. The ITS sequences of and species exhibited far greater interspecies differences than the 18S rRNA sequences and were better than 18S rRNA sequences for measuring close genealogical relationships. Despite the existence of interstrain ITS sequence variation in some species, it is possible to identify conserved regions in both ITSs that are useful in species differentiation.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-46-1-189
1996-01-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/46/1/ijs-46-1-189.html?itemId=/content/journal/ijsem/10.1099/00207713-46-1-189&mimeType=html&fmt=ahah

References

  1. Barnett J. A., Payne R. W., Yarrow D. 1990 Yeasts: characteristics and identification, 2nd. Cambridge University Press; Cambridge:
    [Google Scholar]
  2. Felsenstein J. 1993 PHYLIP: phylogenetic inference package, version 3,5 University of Washington; Seattle:
    [Google Scholar]
  3. Feng D. F., Doolittlte R. F. 1987; Progressive sequence alignment as a prerequisite to correct phylogenetic trees. J. MoL Evol 35:351–360
    [Google Scholar]
  4. Genetics Computer Group 1991 Program manual for the GCG package, version 7 Genetics Computer Group; Madison, Wis:
    [Google Scholar]
  5. Hori H., Osawa S. 1979; Evolutionary change in 5s RNA secondary structure and a phylogenetic tree of 54 5S RNA species. Proc. Natl. Acad. Sci. USA 76:381–385
    [Google Scholar]
  6. Hutson R. A., Thompson D. E., Collins M. D. 1993; Genetic interrelationships of saccharolytic Clostridium botulinum types B, E and F and related Clostridia as revealed by small-subunit rRNA gene sequences. FEMS Microbiol. Lett 108:103–110
    [Google Scholar]
  7. James S. A., Collins M. D., Roberts I. N. 1994; Genetic interrelationship among species of the genus Zygosaccharomyces as revealed by smallsubunit rRNA gene sequences. Yeast 10:871–881
    [Google Scholar]
  8. Kurtzman C. P. 1990; DNA relatedness among species of the genus Zygosaccharomyces. Yeast 6:213–219
    [Google Scholar]
  9. Kurtzman C. P., Robnett C. J. 1991; Phylogenetic relationships among species of Saccharomyces, Schizosaccharomyces, Debaryomyces and Schwanniomyces determined from partial ribosomal RNA sequences. Yeast 7:61–72
    [Google Scholar]
  10. Lott T. J., Kuykendall R. J., Reiss E. 1993; Nucleotide sequence analysis of the 5.8S rDNA and adjacent ITS2 region of Candida albicans and related species. Yeast 9:1199–1206
    [Google Scholar]
  11. Musters W., Boon K., van der Sande C. A. F. M., van Heerikhuizen H., Planta R. J. 1990; Functional analysis of transcribed spacers of yeast ribosomal DNA. EMBO J 9:3989–3996
    [Google Scholar]
  12. O’onnell K. 1992; Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambicum (Gibberella pulicaris). Curr. Genet 22:213–220
    [Google Scholar]
  13. Price C. W., Fuson G. B., Phaff H. J. 1978; Genome comparison in yeast systematics: delimitation within the genera Schwanniomyces, Saccharomyces, Debaryomyces and Pichia. Microbiol, Rev 42:161–193
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. EvoL 4:406–425
    [Google Scholar]
  15. Swofford D. L., Olsen G. 1990 Phylogenetic reconstruction. 411–501 Hillis D. M., Moritz C.ed Molecular systematics Sinauer Associates, Inc.; Publishers, Sunderland, Mass:
    [Google Scholar]
  16. van der Sande C. A. F. M., Kwa M., van Nues R. W., van Heerikhuizen H., Raue H. A., Planta R. J. 1992; Functional analysis of internal transcribed spacer 2 of Saccharomyces cerevisiae ribosomal DNA. J. Mol. Biol 223:899–910
    [Google Scholar]
  17. White T. J. M., Bruns T. D., Lee S., Taylor J. W. 1990 Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. 315–322 Innis M., Gelfand D. H, Sninsky J. J, White T. J.ed PCR protocols Academic Press; San Diego, Calif:
    [Google Scholar]
  18. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev 51:221–271
    [Google Scholar]
  19. Yarrow D. 1984 Zygosaccharomyces Barker. 449–465 Kregervan Rij N. J. W.ed The yeasts: a taxonomic study, 3rd. Elsevier Science Publishers; Amsterdam:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-46-1-189
Loading
/content/journal/ijsem/10.1099/00207713-46-1-189
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error