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Volume 46, Issue 1

sp. nov., a New Thermophilic Anaerobic Bacterium Isolated from Nonvolcanically Heated Geothermal Waters of the Great Artesian Basin of Australia Free

Abstract

A new thermophilic, carbohydrate-fermenting, obligately anaerobic bacterial species was isolated from a runoff channel formed from flowing bore water from the geothermally heated aquifer of the Great Artesian Basin of Australia. The cells of this organism were nonsporulating, motile, gram negative, and rod shaped and generally occurred singly or in pairs. The optimum temperature for growth was 65 to 68°C, and no growth occurred at temperatures below 44°C or above 80°C. Growth was inhibited by 10 μg of lysozyme per ml, 10 μg of penicillin per ml, 10 μg of tetracycline per ml, 10 μg of phosphomycin per ml, 10 μg of vancomycin per ml, and NaCl concentrations greater than 0.2%. The optimum pH for growth was 7.0, and no growth occurred at pH 5.5 or 8.5. The DNA base composition was 35 mol% guanine plus cytosine, as determined by thermal denaturation. The end products of glucose fermentation were lactate, acetate, ethanol, CO, and H. Sulfur, but not thiosulfate, sulfite, or sulfate, was reduced to sulfide. Phase-contrast microscopy of whole cells and an electron microscopic examination of thin sections of cells revealed the presence of single terminal spheroids, a trait common in members of the genus . However, a phylogenetic analysis of the 16S rRNA sequence revealed that the new organism could not be assigned to either of the two previously described species. On the basis of these observations, we propose that the new organism should be designated a new species, . The type strain of this species is strain AB39 (= Australian Collection of Microorganisms strain ACM 5017).

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Copyright © 1996 International Union of Microbiological Societies
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1996-01-01
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References

  1. Davey M. E., Wood W. A., Key R., Nakamura K., Stahl D. A. 1993; Isolation of three species of Geotoga and Petrotoga: two new genera representing a new lineage in the bacterial line of descent distantly related to the “Thermotogalesy”. Syst. Appl. Microbiol 16:191–200
     [Google Scholar]
  2. Denman S., Hampson K., Patel B. K. C. 1991; Isolation of strains of Thermus aquaticus from the Australian Artesian Basin and a simple and rapid procedure for the preparation of their plasmids. FEMS Microbiol. Lett 82:73–78
     [Google Scholar]
  3. Driscoll F. G. 1986 Groundwater and wells, 2nd. Johnson Division; St. Paul, Minn:
     [Google Scholar]
  4. Felsenstein J. 1993 PHYLIP (phylogenetic inference package), version 3.51c Department of Genetics; University of Washington, Seattle:
     [Google Scholar]
  5. Habermehl M. A. 1980; The Great Artesian Basin, Australia. BMR (Bur. Miner. Resour.) J. Aust. Geol. Geophys 5:9–38
     [Google Scholar]
  6. Huber R., Woese C. R., Langworthy T. A., Kristjansson J. K., Stetter K. O. 1990; Fervidobacterium islandicum sp. nov., a new extremely thermophilic eubacterium belonging to the “Thermotoga” Arch. Microbiol 154:105–111
     [Google Scholar]
  7. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism Academic Press; New York:
     [Google Scholar]
  8. Larsen N., Olsen G. J., Maidak B. L., McCaughey M. J., Overbeek R., Macke T. J., Marsh T. L., Woese C. R. 1993; The Ribosomal Database Project. Nucleic Acids Res 21:3021–3023
     [Google Scholar]
  9. Love C. A., Patel B. K. C., Nichols P. D., Stackebrandt E. 1993; Desulfotomaculum australicum, sp. nov., a thermophilic sulfate-reducing bacterium isolated from the Great Artesian Basin of Australia. Syst. Appl. Microbiol 16:244–251
     [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from bacteria. J. Mol. Biol 3:208–218
     [Google Scholar]
  11. Patel B. K. C., Morgan H. W., Daniel R. M. 1985; A simple and efficient method for preparing and dispensing anaerobic media. Biotechnol. Lett 7:277–288
     [Google Scholar]
  12. Patel B. K. C., Morgan H. W., Daniel R. M. 1985; Fervidobacterium nodosum gen. nov. and spec, nov., a new chemoorganotrophic, caldoactive-anaerobic bacterium. Arch. Microbiol 141:63–69
     [Google Scholar]
  13. Patel B. K. C., Skerratt J. H., Nichols P. D. 1991; The phospho ester-linked fatty acid composition of thermophilic bacteria. Syst, Appl. Microbiol 14:311–316
     [Google Scholar]
  14. Philips W. E., Perry J. J. 1976; Thermomicrobium fosteri sp. nov., a hydrocarbon-utilizing obligate thermophile. Int. J. Syst. Bacteriol 26:220–225
     [Google Scholar]
  15. Ramaley R. F., Bitzinger K. 1975; Types and distribution of obligate thermophilic bacteria in man-made and natural gradients. Appl. Microbiol 30:152–155
     [Google Scholar]
  16. Ravot G., Magot M., Fardeau M. L., Patel B. K. C., Prensier G., EgJUh A., Garcia J. L., Ollivier B. 1995; Thermotoga elfi sp. nov., a novel thermophilic bacterium from an African oil-producing well. Int. J. Syst. Bacteriol 45:308–314
     [Google Scholar]
  17. Redburn A. C., Patel B. K. C. 1993; Phylogenetic analysis of Desulfotomaculum thermobenzoicum using polymerase chain reaction-amplified 16S rRNA-specific DNA. FEMS Microbiol. Lett 113:81–86
     [Google Scholar]
  18. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol 44:846–849
     [Google Scholar]
  19. Stetter K. O., Fiala G., Huber G., Huber R., Segerer A. 1990; Hyperthermophilic microorganisms. FEMS Microbiol. Rev 75:117–124
     [Google Scholar]
  20. Van de Peer Y., De Wachter R. 1992; TREECON: a software package for the construction and drawing of evolutionary trees. Comput. AppL Biosci 9:177–182
     [Google Scholar]
  21. Winker S., Woese C. R. 1991; A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA characteristics. Syst. Appl. Microbiol 14:305–310
     [Google Scholar]
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Fervidobacterium gondwanense sp. nov., a New Thermophilic Anaerobic Bacterium Isolated from Nonvolcanically Heated Geothermal Waters of the Great Artesian Basin of Australia
Int J Syst Evol Microbiol 46, 265 (1996); https://doi.org/10.1099/00207713-46-1-265
/content/journal/ijsem/10.1099/00207713-46-1-265
/content/journal/ijsem/10.1099/00207713-46-1-265
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