1887

Abstract

A polyphasic taxonomic study of four strains of and four strains of (including duplicates of both type strains) supported the reclassification of both former species into one species, . Our conclusions were based on morphological and Analytab Products (API) tests, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins, gas chromatography of methylated fatty acids, pyrolysis mass spectrometry, DNA-DNA binding, and the following genomic fingerprinting methods: amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and AFLP analysis. The last method is a novel high-resolution DNA fingerprinting technique based on the selective amplification of restriction fragments. Despite more than 90% DNA relatedness between the strains studied, SDS-PAGE of whole-cell proteins, biochemical tests, and DNA fingerprinting (AFLP) distinguished between the and strains at the subspecies level. Taking this evidence along with differences in pathogenicity, we propose to reclassify the honeybee pathogens and as subsp. and subsp. . An emended description of the species and descriptions of the subspecies are given. The type strains are subsp. ATCC 9545 (LMG 9820) and subsp. NRRL B-3685 (LMG 6911 and ATCC 13537).

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1996-01-01
2024-03-28
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References

  1. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett. Appl. Microbiol 13:202–206
    [Google Scholar]
  2. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek J. Microbiol 64:253–260
    [Google Scholar]
  3. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol 101:738–754
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem 12:133–142
    [Google Scholar]
  5. De Ley J., Van Muylem J. 1963; Some applications of deoxyribonucleic acid base composition in bacterial taxonomy. Antonie van Leeuwenhoek J. Microbiol. Serol 29:344–358
    [Google Scholar]
  6. Dingman D. W., Stahly D. P. 1983; Medium promoting sporulation of Bacillus larvae and metabolism of medium components. Appl. Environ. Microbiol 46:860–869
    [Google Scholar]
  7. Drobnikova V., Richter V., Hausler J., Pytelova I. 1994; Characterization of Bacillus larvae and related bacilli by chromatography of cell fatty acids. J. Apic. Res 33:69–74
    [Google Scholar]
  8. Goodacre R., Berkeley R. C. W. 1990; Detection of small genotypic changes in Escherichia coll by pyrolysis mass spectrometry. FEMS Microbiol. Lett 71:133–138
    [Google Scholar]
  9. Gordon R. E., Haynes W. C., Pang C. H.-N. 1973 The genus Bacillus. Agriculture handbook no. 427. U.S. Department of Agriculture; Washington, D.C:
    [Google Scholar]
  10. Gutteridge C. S. 1987; Characterization of microorganisms by pyrolysis mass spectrometry. Methods Microbiol 19:227–272
    [Google Scholar]
  11. Gutteridge C. S., Vallis L., MacFie H. J. H. 1985 Numerical methods in the classification of micro-organisms by pyrolysis mass spectrometry. 369–401 Goodfellow M., Jones D., Priest F. G.ed Computer assisted bacterial systematics Academic Press; New York:
    [Google Scholar]
  12. Heyndrickx M., Vandemeulebroecke K., Scheldeman P., Hoste B., Kersters K., De Vos P., Logan N. A., Aziz A. M., Ali N., Berkeley R. C. W. 1995; Paenibacillus (formerly Bacillus) gordonae (Pichinoty ct al. 1986) Ash et al. 1994 is a later subjective synonym of Paenibacillus (formerly Bacillus) validus (Nakamura 1984) Ash et al. 1994: emended description of P. validus. Int. J. Syst. Bacteriol 45:661–669
    [Google Scholar]
  13. Hughes M. S., Skuce R. A., Beck L.-A., Neill S. D. 1993; Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences. J. Clin. Microbiol 31:3216–3222
    [Google Scholar]
  14. International Committee on Systematic Bacteriology 1993; Minutes of Subcommittee on the Taxonomy of the Genus Bacillus. Int. J, Syst. Bacteriol 43:185
    [Google Scholar]
  15. Janssen P. Universiteit Gent; 1995 Personal communication
  16. Katznelson H. 1950; Bacillus pulvifaciens (n. sp.), an organism associated with powdery scale of honeybee larvae. J. BacterioL 59:153–155
    [Google Scholar]
  17. Logan N. A. 1994 Bacterial systematics Blackwell Scientific Publications; Oxford:
    [Google Scholar]
  18. Logan N. A., Berkeley R. C. W. 1984; Identification of Bacillus strains using the API system. J. Gen. Microbiol 130:1871–1882
    [Google Scholar]
  19. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. BioL 3:208–218
    [Google Scholar]
  20. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. MoL Biol 5:109–118
    [Google Scholar]
  21. Nakamura L. K. 1984; Bacillus pulvifaciens sp. nov., nom. rev. Int. J. Syst. BacterioL 34:410–413
    [Google Scholar]
  22. Nei M., Li W.-H. 1979; Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76:5269–5273
    [Google Scholar]
  23. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacteria] genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol 8:151–156
    [Google Scholar]
  24. Pot B., Vandamme P., Kersters K. 1994 Analysis of electrophoretic whole organism protein fingerprints. 493–521 Goodfellow Az M., O’Donnell A. G.ed Chemical methods in prokaryotic systematics J. Wiley & Sons; Chichester, United Kingdom:
    [Google Scholar]
  25. Skerman V. B. D., McGowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. Int. J. Syst. BacterioL 30:225–420
    [Google Scholar]
  26. Vaneechoutte M., Rossau R., De Vos P., Gillis M., Janssens D., Paepe N., De Rouck A., Fiers T., Claeys G., Kersters K. 1992; Rapid identification of bacteria of the Comamonadaceae with amplified ribosomal DNA-restriction analysis (ARDRA). FEMS Microbiol. Lett 93:227–234
    [Google Scholar]
  27. Vauterin L., Vauterin P. 1992; Computer aided objective comparison of electrophoresis patterns for grouping and identification of microorganisms. Eur. Microbiol 1:37–41
    [Google Scholar]
  28. Vauterin L., Yang P., Hoste B., Vancanneyt M., Civerolo E. L., Swings J., Kersters K. 1991; Differentiation of Xanthomonas campestris pv. citri strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins, fatty acid analysis, and DNA-DNA hybridization. Int. J. Syst. BacterioL 41:535–542
    [Google Scholar]
  29. Vos P., Hogers R., Bleeker M., Reijans M., van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M., Zabeau M. 1995; AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
    [Google Scholar]
  30. White G. F. 1906 The bacteria of the apiary, with special reference to bee diseases Bureau of Entomology Technical Series no. 14. U.S. Department of Agriculture; Washington, D.C:
    [Google Scholar]
  31. Willems A., Busse J., Goor M., Pot B., Falsen E., Jantzen E., Hoste B., Gillis M., Kersters K., Auling G., De Ley J. 1989; Hydrogenophaga, a new genus of hydrogen-oxidizing bacteria that includes Hydrogenophaga flava comb. nov. (formerly Pseudomonas flava), Hydrogenophaga palleronii (formerly Pseudomonas palleronii), Hydrogenophaga pseudoftava (formerly Pseudomonas pseudoflava and “Pseudomonas carboxydofla”), and Hydrogenophaga taeniospiralis (formerly Pseudomonas taemospiralis). Int. J. Syst. BacterioL 39:319–333
    [Google Scholar]
  32. Willems A., Gillis M., De Ley J. 1991; Transfer of Rhodocyclus gelatinosus to Rubrivivax gelatinosus gen. nov., comb, nov., and phylogenetic relationships with Leptothrix, Sphaerotilus natans, Pseudomonas saccharophila, and Alcaligenes latus. Int. J. Syst. BacterioL 41:65–73
    [Google Scholar]
  33. Zabeau M., Vos P. 1993; Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Office publication 0 534 858
    [Google Scholar]
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