1887

Abstract

A study of DNA reassociation kinetics and electrophoretic karyotypes of several strains classified as , and strains revealed that the group included members of at least five distinct species. Two of the four strains classified as exhibited very high levels of DNA sequence homology with the type strain of , while the type strain and another strain demonstrated intermediate levels of relatedness to the type strains of the unrelated taxa and . Five strains that were classified as strains were not related to the type strains of the taxa studied. Two of these strains exhibited intermediate relatedness (65% base sequence homology), while another pair exhibited a level of DNA reassociation of 97%. The remaining strain was distinct from all the other strains studied on the basis of both DNA base sequences and electrophoretic karyotype data.

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1996-01-01
2024-04-24
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References

  1. Bernardi G., Faures M., Piperno G., Slonimski P. P. 1970; Mitochondrial DNAs from respiratory-sufficient and cytoplasmic respiratoiy-deficient mutants of yeasts. J. Mol. Biol 48:23–43
    [Google Scholar]
  2. Capriotti A. 1966; Saccharomyces castellii n. sp.: una nuova specie di lievito isolata da un terreno della Finlandia. Ann. Fac. Agrar. Sassari 14:453–458
    [Google Scholar]
  3. Casey G. P., Xiao W., Rank G. H. 1988; Application of pulsed field chromosome electrophoresis in the study of chromosome XIII and the electrophoretic karyotype of industrial strains of Saccharomyces yeasts. J. Inst. Brew 94:239–243
    [Google Scholar]
  4. Johnston J. R., Mortimer R. K. 1986; Electrophoretic karyotyping of laboratory and commercial strains of Saccharomyces and other yeasts. Int. J. Syst. Bacteriol 36:569–572
    [Google Scholar]
  5. Kaneko Y., Banno I. 1991; Reexamination of Saccharomyces bay anus strains by DNA-DNA hybridization and electrophoretic karyotyping. Inst. Ferment. Res. Commun, (Osaka) 15:30–41
    [Google Scholar]
  6. Kudriavzev V. L. 1960 Die Systematik der Hefen Akademie Verlag; Berlin:
    [Google Scholar]
  7. Kurtzman C. P. Personal communication
  8. Kurtzman C. P., Phaff H. J. 1987 Molecular taxonomy. 63–94 Rose A. H., Harrison J. S.ed The yeasts: biology of yeasts 1 Academic Press; London:
    [Google Scholar]
  9. Kurtzman C. P., Robnett C. J. 1991; Phylogenetic relationships among species of Saccharomyces, Schizosaccharomyces. Debaiyomyces and Schwanniomyces determined from partial ribosomal RNA sequences. Yeast 7:61–72
    [Google Scholar]
  10. Kurtzman C. P., Smiley M. J., Johnson C. J. 1980; Emendation of the genus Issatchenkia Kudriavzev and comparison of species by deoxyribonucleic acid reassociation, mating reaction, and ascospore ultrastructure. Int. J. Syst. Bacteriol 30:503–513
    [Google Scholar]
  11. Kurtzman C. P., Smiley M. J., Johnson C. J., Fuson G. B., Wickerham L. J. 1980; Two new closely related heterothallic species, Pichia amylophilia and Pichia mississippiensis\ characterization by hybridization and deoxyribonucleic acid reassociation. Int. J. Syst. Bacteriol 30:208–216
    [Google Scholar]
  12. Lodder J., Kreger-van Rij N. J. W. 1952 The yeasts–a taxonomic study North-Holland; Amsterdam:
    [Google Scholar]
  13. Meyer S. A., Smith M. T., Simione F. P. 1978; Systematics of Hanseniaspora Zikes and Kloeckera Janke. Antonie Leeuwenhoek 44:79–96
    [Google Scholar]
  14. Naganishi H. 1917; Three new species of yeasts. Bot. Mag. Tokyo 31:107–115
    [Google Scholar]
  15. Naumov G., Naumova E., Lantto R. A., Louis E. J., Korhola M. 1992; Genetic homology between Saccharomyces cerevisiae and its sibling species S. paradoxus and S. bayamis: electrophoretic karyotypes. Yeast 8:599–612
    [Google Scholar]
  16. Rank G. H., Casey G., Xiao W. 1988; Gene transfer in industrial Saccharomyces yeasts. Food Biotechnol 2:1–41
    [Google Scholar]
  17. Stelling-Dekker N. M. 1931; Die sporogenen Hefen. Vcrh. K. Ned. Akad. Wet. Afd. Natuurk 28:1–547
    [Google Scholar]
  18. van der Walt J. P. 1956; Saccharomyces transvaalensis nov. spec. A new yeast from soil. Antonie Leeuwenhoek 22:190–192
    [Google Scholar]
  19. van der Walt J. P. 1970 Saccharomyces emend. Reess. 555–718 Lodder J.ed The yeasts-a taxonomic study Elsevier Science Publishers; Amsterdam:
    [Google Scholar]
  20. van der Walt J. P. 1978; The genus Pachytichospora gen. nov. Bothalia 12:563–564
    [Google Scholar]
  21. van der Walt J. P., Liedenberg N. V. D. W. 1973; Agglutinative mating types of Saccharomyces transvaalensis. Antonie Leeuwenhoek 39:629–633
    [Google Scholar]
  22. van Uden N., do Carmo-Sousa L. 1962; On the intestinal yeast flora of free living baboons (Papio cynocephalus). Sabouraudia 2:8–11
    [Google Scholar]
  23. Vaughan-Martini A. 1989; Saccharomyces paradoxus comb. nov., a newly separated species of the Saccharomyces sensu stricto complex based upon nDNA/nDNA homologies. Syst. Appl. MicrobioL 12:179–182
    [Google Scholar]
  24. Vaughan-Martini A. Unpublished data
  25. Vaughan-Martini A., Kurtzman C. P. 1985; Deoxyribonucleic acid relatedness among species of the genus Saccharomyces sensu stricto. Int. J. Syst. Bacteriol 35:508–511
    [Google Scholar]
  26. Vaughan-Martini A., Kurtzman C. P. 1988; Deoxyribonucleic acid relatedness among species of Saccharomyces sensu lato. Mycologia 80:241–243
    [Google Scholar]
  27. Vaughan-Martini A., Martini A. 1987; Three newly delimited species of Saccharomyces sensu stricto. Antonie van Leeuwenhoek J. Microbiol. Serol 53:77–84
    [Google Scholar]
  28. Vaughan-Martini A., Martini A., Cardinall G. 1993; Electrophoretic karyotyping as a taxonomic tool in the genus Saccharomyces. Antonie Leeuwenhoek 63:145–156
    [Google Scholar]
  29. Yarrow D. 1984 Saccharomyces Meyer ex Reess. 379–395 Kreger-van Rij N. J. W.ed The yeasts: a taxonomic study, 3rd. Elsevier Science Publishers B.V.; Amsterdam:
    [Google Scholar]
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