1887

Abstract

, and (M.-C. Curk, J.-C. Hubert, and F. Bringel, Int. J. Syst. Bacteriol. 46:595–598, 1996) can hardly be distinguished on the basis of their phenotypes. Unlike and ferments glycerol and xylose but not melezitose. We identified two strains (CNRZ 1538 and CNRZ 1544) which ferment glycerol and melezitose but not xylose. α-Methyl--mannoside was fermented by 66% of the strains tested but not by strains. In this paper we describe a simple method to identify , and . This method is based on nonradioactive Southern-type hybridization between I DNA digests of the lactobacilli tested and a DNA probe ( genes from strain CCM 1904). A total of 68 lactobacilli were classified into five groups on the basis of the bands detected. Two groups contained strains; one of these groups contained 31 strains, including the type strain, and was characterized by bands at 7, 4, and 1 kb, and the other group contained strain LP 85-2 and was characterized by bands at 5 and 1.1 kb. Only one band (a band at around 7 kb) was detected in the strains belonging to the group, and two bands (at 4 and 1 kb) were found in the strains belonging to the group. No hybridization was detected in the last group, which contained , and strains.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-46-2-588
1996-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/46/2/ijs-46-2-588.html?itemId=/content/journal/ijsem/10.1099/00207713-46-2-588&mimeType=html&fmt=ahah

References

  1. Abdi A. 1992 Contribution á I’étude de la chaine de biosynthese de novo des nucleotides pyrimidiques chez Lactobacillus plantarum. These de Doctorat- Domaine de Biologie Cellulaire et Moleculaire. Universite Louis-Pasteur de Strasbourg; Strasbourg, France:
    [Google Scholar]
  2. Bouia A., Bringel F., Frey L., Belarbi A., Guyonvarch A., Kammerer B., Hubert J.-C. 1990; Cloning and structure of the pyrE gene of Lactobacillus plantarum CCM 1904. FEMS Microbiol. Lett 69:233–238
    [Google Scholar]
  3. Champomier M.-C., Montel M.-C., Talon R. 1989; Nucleic acid relatedness studies on the genus Camobacterium and related taxa. J. Gen. Microbiol 135:1391–1394
    [Google Scholar]
  4. Chevallier B., Hubert J.-C., Kammerer B. 1994; Determination of chromosome size and number of rm loci in Lactobacillus plantamm by pulsed-field gel electrophoresis. FEMS Microbiol. Lett 120:51–56
    [Google Scholar]
  5. Collins M. D., Phillips B. A., Zanoni P. 1989; Deoxyribonucleic acid homology studies of Lactobacillus casei, Lactobacillus paracasei sp. nov., subsp. paracasei and subsp. tolerans, and Lactobacillus rhamnosus sp. nov., comb. nov. Int. J. Syst. Bacteriol 39:105–108
    [Google Scholar]
  6. Collins M. D., Rodrigues U., Ash C., Aguirre M., Farrow J. A. E., Martinez-Murcia A., Phillips B. A., Williams A. M., Wallbanks S. 1991; Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA. FEMS Microbiol. Lett 77:5–12
    [Google Scholar]
  7. Curk M.-C., Hubert J.-C., Bringel F. 1996; Lactobacillus paraplantarum sp. nov., a new species related to Lactobacillus plantamm. Int. J. Syst. Bacteriol 46:595–598
    [Google Scholar]
  8. Curk M.-C., Peladan F., Hubert J.-C. 1994; Fourier transform infrared (FTIR) spectroscopy for identifying Lactobacillus species. FEMS Microbiol. Lett 123:241–248
    [Google Scholar]
  9. Dellaglio F., Bottazzi V., Vescovo M. 1975; Deoxyribonucleic acid homology among Lactobacillus species of the subgenus Streptobacterium Orla-Jensen. Int. J. Syst. Bacteriol 25:160–172
    [Google Scholar]
  10. Fred E. B., Peterson W. H., Anderson J. A. 1921; The characteristics of certain pentose-destroying bacteria, especially as concerns their action on arabinose and xylose. J. Biol. Chem 48:385–412
    [Google Scholar]
  11. Grimont P. A. D. 1984 DNA/DNA hybridization in bacterial taxonomy. 11–19 Sanna A, Morace G.ed New horizons in microbiology Elsevier Science Publishers B. V.; Amsterdam:
    [Google Scholar]
  12. Grimont P. A. D., Popoff M. Y., Grimont F., Coynault C., Lemelin M. 1980; Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures. Curr. Microbiol 4:325–330
    [Google Scholar]
  13. Kandler O., Weiss N. 1986 Regular, nonsporing Gram-positive rods, 1208–1234. Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology 2 The Williams and Wilkins Co.; Baltimore:
    [Google Scholar]
  14. Lee L.-J., Hansen J. B., Jagusztyn-Krynicka E. K., Chassy B. M. 1982; Cloning and expression of the p-d-phosphogalactoside galactohydrolase gene of Lactobacillus easel in Escherichia coll K-12. J. Bacteriol 152:1138–1146
    [Google Scholar]
  15. Lokman B. C., van Santen P., Verdoes J. C., Kriise J., Leer R. J., Posno M., Pouwels P. H. 1991; Organization and characterization of three genes involved in d-xylose catabolism in Lactobacillus pentosus. Mol. Gen. Genet 230:161–169
    [Google Scholar]
  16. Molin G., Jeppsson B., Johansson M.-L., Ahrne S., Nobaek S., Stahl M., Bengmark S. 1993; Numerical taxonomy of Lactobacillus spp. associated with healthy and diseased mucosa of the human intestines. J. AppL Bacteriol 74:314–323
    [Google Scholar]
  17. Rose A. H.ed 1982 Economic microbiology, fermented foods 7 Academic Press; London:
    [Google Scholar]
  18. Ruiz-Barba J. L., Piard J. C., Jimenez-Diaz R. 1991; Plasmid profiles and curing of plasmids in Lactobacillus plantamm strains isolated from green olive fermentations. J. AppL Bacteriol 71:417–421
    [Google Scholar]
  19. Sharpe M. E., Pettipher G. L. 1983 Food spoilage by lactic-acid bacteria. 199–223 Rose A. H.ed Economic microbiology, food microbiology 8 Academic Press; London:
    [Google Scholar]
  20. Southern E. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol 98:513–517
    [Google Scholar]
  21. Tamura C., Matsushita O. 1992; Melibiose transport system in Lactobacillus plantarum. Microbiol. ImmunoL 36:1119–1128
    [Google Scholar]
  22. Vanderslice P., Copeland W. C., Robertus J. D. 1986; Cloning and nucleotide sequence of wild type and a mutant histidine decarboxylase from Lactobacillus 30a. J. Biol. Chem 261:15186–15191
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol 37:463–464
    [Google Scholar]
  24. Zanoni P., Farrow J. A. E., Phillips B. A., Collins M. D. 1987; Lactobacillus pentosus (Fred, Peterson, and Anderson) sp. nov., nom. rev. Int. J. Syst. Bacteriol 37:339–341
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-46-2-588
Loading
/content/journal/ijsem/10.1099/00207713-46-2-588
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error