1887

Abstract

In order to establish the taxonomic value of 16S rRNA and 23S rRNA for distinguishing species, the complete 23S rRNA sequences for all species were determined by using the type strains. We designed and experimentally validated a universal 23S rRNA sequencing method, which included PCR amplification of the rDNA gene and direct cycle sequencing of the amplicon with eubacterial primers. The results of our sequence comparison indicated that the genus can be divided into two subgroups; one subgroup is composed of , and , whereas the other subgroup includes subsp. and subsp. A phylogenetic analysis revealed that these species diverged recently. These results are consistent with 16S rRNA sequence analysis data. For application purposes, one 16S rRNA region that can be used to distinguish each species except and has been described. In this study we found four 23S rRNA signature regions which, when used in combination, can be used to distinguish the species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-46-3-669
1996-07-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/46/3/ijs-46-3-669.html?itemId=/content/journal/ijsem/10.1099/00207713-46-3-669&mimeType=html&fmt=ahah

References

  1. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal sequences. Lett. Appl. Microbiol 13:202–206
    [Google Scholar]
  2. bioMerieux S.A. February 1995 French patent 95 01431
  3. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. 1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coll. J. Mol. Biol 148:107–127
    [Google Scholar]
  4. Cilia V., Lafay B., Christen R. Sequence heterogeneity exists among the 16S ribosomal RNA sequences of the seven operons in Escherichia coli strain PK3 that can affect phylogenetic analyses at the species level. Mol. Biol. EvoL in press
    [Google Scholar]
  5. Collins M. D., Wallbanks S., Lane D. J., Shah J., Nietupski R., Smida J., Dorsch M., Stackebrandt E. 1991; Phylogenetic analysis of the genus Listeria based on reverse transcriptase sequencing of 16S rRNA. Int. J. Syst. Bacterio1 41:240–246
    [Google Scholar]
  6. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism Academic Press; New York:
    [Google Scholar]
  7. Kimura M. 1968; Evolutionary rate at the molecular level. Nature (London) 217:624–626
    [Google Scholar]
  8. Ludwig W., Kirchhof G., Klugbauer N., Weizenegger M., Betzl D., Ehrmann M., Hertel C., Jilg S., Tatzel R., Zitzelsberger H., Liebl S., Hochbeier M., Shah J., Lane D., Wallndfer P. R., Schleifer K. H. 1992; Complete 23S ribosomal RNA sequences of Gram-positive bacteria with a low DNA G+C content. Syst. Appl. Microbiol 15:487–701
    [Google Scholar]
  9. Rocourt J., Boerlin P., Grimont F., Jacquet C., Piffaretti J. C. 1992; Assignment of Listeria grayi and Listeria murrayi to a single species, Listeria grayi, with a revised description of Listeria grayi. Int. J. Syst. Bacteriol 42:171–174
    [Google Scholar]
  10. Rossler D., Ludwig W., Schleifer K. H., Lin C., McGill T. J., Wisotzkey J. D., Jurtshuk P., Fox G. E. 1991; Phylogenetic diversity in the genus Bacillus as seen by 16S RNA sequencing studies. Syst. Appl. Microbiol 14:266–269
    [Google Scholar]
  11. Ruimy R., Riegel P., Boiron P., Monteil H., Christen R. 1995; Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences. Int. J. Syst. Bacteriol 45:740–746
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. EvoL 4:406–425
    [Google Scholar]
  13. Sallen B., Rajoharison A., Desvarenne S., Mabilat C. 1995; Molecular epidemiology of integron-associated antibiotic resistance genes in clinical isolates of Enterobacteriaceae. Microb. Drug Resist 1:195–202
    [Google Scholar]
  14. Swoflfbrd D. 1990 PAUP: phylogenetic analysis using parsimony, version 3.0 Illinois Natural History Survey; Champaign:
    [Google Scholar]
  15. Thompson D. E., Balsdon J. T., Cai J., Collins M. D. 1992; Studies on the ribosomal RNA operons of Listeria monocytogenes. FEMS Microbiol. Lett 75:219–224
    [Google Scholar]
  16. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev 51:221–227
    [Google Scholar]
  17. Zuckerkandl E., Pauling L. 1965; Molecules as documents of evolutionary history. J. Theor. Biol 8:357–366
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-46-3-669
Loading
/content/journal/ijsem/10.1099/00207713-46-3-669
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error