1887

Abstract

The name Katznelson 1955 was not included on the Approved Lists of Bacterial Names and thus lost standing in bactenal nomenclature. The genetic homogeneity of strains was assessed by determining their G+C contents by the buoyant density method and by measuring the levels of DNA relatedness by spectrophotometric reassociation procedures. The G+C contents of the 15 strains examined, ranged from 52 to 54 mol%. DNA reassociation revealed the presence of two clusters each with high levels of intragroup relatedness (60 to 100%). One cluster consisted of six strains highly related to , and the other consisted of nine strains related to the designated type strain of The strains in the second cluster were not closely related genetically to the type strains of organisms frequently associated with honey bees (namely, , and ). The strains in the second cluster were also phenotypically homogeneous and distinguishable from the previously described species. Comparative analyses of the 16S rRNA gene DNA sequence showed that the proper phylogenetic position of the second cluster was in the genus . These findings justify the proposal of a new species with the name . The type strain is NRRL NRS-1438.

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1996-07-01
2024-04-25
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References

  1. Ash C., Farrow J. A. E., Walksbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-ribosomal RNA sequences. Lett. AppL Microbiol 13:202–206
    [Google Scholar]
  2. Ash C, Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Walksbanks, and Collins) using a PCR probe test. Antonie Leeuwenhoek 64:253–260
    [Google Scholar]
  3. Claus D., Berkeley R. C. W. 1986 Genus Bacillus Cohn 1872. 1105–1139 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology 2 Williams & Wilkins; Baltimore:
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem 12:133–142
    [Google Scholar]
  5. Gordon R. E., Haynes W. C., Pang C. H.-N. 1973 The genus Bacillus Agricultural Handbook no. 427. U.S. Department of Agriculture; Washington, D.C:
    [Google Scholar]
  6. Haynes W. C., Wickeriiam L. J., Hesseltine C. W. 1955; Maintenance of cultures of industrially important microorganisms. AppL Microbiol 3:361–368
    [Google Scholar]
  7. Higgines D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment. Comput. Appl. Biosci 8:189–191
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism Academic Press; New York:
    [Google Scholar]
  9. Katznelson H. 1955; Bacillus apiarius n. sp., an aerobic spore-forming organism isolated from honey bee larvae. J. Bacteriol 70:635–636
    [Google Scholar]
  10. Lane D. L. 1991 16S/23S rRNA sequencing. 115–175 Stackebrandt E., Goodfellow M.ed Nucleic acid techniques in bacterial systematics John Wiley & Sons; New York:
    [Google Scholar]
  11. Larsen N. A., Olsen G. J., Maidak B. L., McCaughey M. J., Overbeek R., Marsh T. J., Woese C. R. 1993; The Ribosomal Database Project. Nucleic Acids Res 21:3021–3023
    [Google Scholar]
  12. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular cloning: a laboratory manual Cold Spring Harbor Laboratory Cold Spring Harbor; N.Y.:
    [Google Scholar]
  13. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  14. Nakamura L. K., Swezey J. 1983; Taxonomy of Bacillus circulans Jordan 1890: base composition and reassociation of deoxyribonucleic acid. Int. J. Syst. Bacteriol 33:46–52
    [Google Scholar]
  15. Olsen G. J. 1988; Phylogenetic analysis using ribosomal RNA. Methods Enzymol 164:793–812
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4:406–425
    [Google Scholar]
  17. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids Technical Note 101. Microbial ID, Inc.; Newark, Del:
    [Google Scholar]
  18. Skerman V. B. D., McGowan V., Sneath P. H. A.ed Approved lists of bacterial names. Int. J. Syst. BacterioL 30:225–420
    [Google Scholar]
  19. Sneath P. H. A., Sokal R. R. 1973 Numerical taxonomy. W. H. Freeman and Co.; San Francisco:
    [Google Scholar]
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