1887

Abstract

The outer membrane protein (OMP) and lipopolysaccharide (LPS) profiles of 30 untypeable isolates of were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and compared with the profiles of typeable isolates. The phylogenetic relationships of 28 isolates representing each of the serotypes of and , as well as untypeable isolates of , were determined by comparing 16S rRNA sequences. The analysis of the OMP and LPS profiles of the untypeable isolates revealed five groups, which were designated untypeable groups 1 (UG1) through UG5. The UG1 and UG2 isolates had OMP and LPS profiles identical to the profiles of certain serotype A1 and A2 isolates, respectively. Furthermore, UG1 isolates originating from cattle and sheep could be clearly differentiated on the basis of their OMP profiles. The OMP and LPS profiles of UG3 isolates were similar in appearance to the profiles of serotype A11 isolates, suggesting that these two groups are closely related. The OMP profiles of UG4 and UG5 isolates were unique and different from the OMP profiles of the UG1 through UG3 isolates. A comparison of 16S rRNA sequences revealed that typeable isolates of could be divided into the following three groups: (i) serotype A1, A5 through A9, A12 through A14, and A16 isolates, (ii) serotype A2 isolates, and (iii) serotype A11 isolates. The isolates belonging to the first group all had identical sequences, whereas the sequences of isolates belonging to the second and third groups differed from the sequences of the isolates belonging to the first group at two and four base positions, respectively. The sequence data for the untypeable isolates confirmed the conclusions derived from the OMP and LPS analysis. Isolates belonging to UG1 and UG2 were identical to serotype A1 and A2 isolates, respectively; isolates belonging to UG3 were related to serotype A11 isolates, although there was some sequence heterogeneity within this group; and isolates belonging to UG4 and UG5 were more distantly related to than were isolates belonging to UG1 through UG3 and were clearly members of two different species. As expected, isolates of were even more distantly related to than were the untypeable isolates, but there was significantly more sequence variation among the four serotypes of this species than there was among the serotypes of . The correlation of the OMP and LPS data with the 16S rRNA sequence data suggested that OMP and LPS analyses might be useful for preliminary screening and comparing large numbers of isolates in taxonomic and epidemiological studies of the .

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1996-07-01
2024-03-28
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References

  1. Aarsleff B., Biberstein E. L., Shreeve B. J., Thompson D. A. 1970; A study of untypable strains of Pasteurella haemolytica. J. Comp. Pathol 80:493–498
    [Google Scholar]
  2. Adlam C. 1989 The structure, function and properties of cellular and extracellular components of Pasteurella haemolytica. 75–92 Adlam C., Rutter M. J.ed Pasteurella and pasteurellosis Academic Press; London:
    [Google Scholar]
  3. Biberstein E. L. 1978; Biotyping and serotyping of Pasteurella haemolytica. Methods Microbiol 10:253–269
    [Google Scholar]
  4. Biberstein E. L., Francis C. K. 1968; Nucleic acid homologies between the A and T types of Pasteurella haemolytica. J. Med. Microbiol 1:105–108
    [Google Scholar]
  5. Bisgaard M. 1984; Comparative investigations of Pasteurella haemolytica sensu stricto and so-called P. haemolytica isolated from different pathological lesions in pigs. Acta Pathol. Microbiol. Immunol. Scand. Sect. B 92:201–207
    [Google Scholar]
  6. Bisgaard M., Mutters R. 1986; Re-investigations of selected bovine and ovine strains previously classified as Pasteurella haemolytica and description of some new taxa within the Pasteurella haemolytica complex. Acta Pathol. Microbiol. Immunol. Scand. Sect. B 94:185–193
    [Google Scholar]
  7. Bisgaard M., Phillips J. E., Mannheim W. 1986; Characterization and identification of bovine and ovine Pasteurellaceae isolated from the oral cavity and rumen of apparently normal cattle and sheep. Acta Pathol. Microbiol. Immunol. Scand. Sect. B 94:9–17
    [Google Scholar]
  8. Clayton R. A., Sutton G., Hinkle P. S., Bult C., Fields C. 1995; Intraspecific variation in small-subunit rRNA sequences in GenBank: why single sequences may not adequately represent prokaryotic taxa. Int. J. Syst. Bacteriol 45:595–599
    [Google Scholar]
  9. Davies R L., Ali Q., Parton R., Coote J. G., Gibbs H. A., Freer J. H. 1991; Optimal conditions for the analysis of Pasteurella haemolytica lipopolysaccharide by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. FEMS Microbiol. Lett 90:23–28
    [Google Scholar]
  10. Davies R. L., Donachie W. 1996; Intra-specific diversity and host specificity within Pasteurella haemolytica based on variation of capsular polysaccharide, lipopolysaccharide and outer membrane proteins. Microbiology 142:1895–1907
    [Google Scholar]
  11. Davies R. L., Parton R., Coote J. G., Gibbs H. A., Freer J. H. 1992; Outer membrane protein and lipopolysaccharide variation in Pasteurella haemolytica Al under different growth conditions. J. Gen. Microbiol 138:909–922
    [Google Scholar]
  12. Davies R. L., Quirie M. 1996; Intra-specific diversity within Pasteurella trehalosi based on variation of capsular polysaccharide, lipopolysaccharide and outer membrane proteins. Microbiology 142:551–560
    [Google Scholar]
  13. Dewhirst F. Er, Paster B. J., Olsen I., Fraser G. J. 1992; Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J. Bacteriol 174:2002–2013
    [Google Scholar]
  14. Donachie W., Fraser J., Quirie M., Gilmour N. J. L. 1984; Studies on strains of Pasteurella haemolytica not typable by the indirect haemagglutination test. Res. Vet. Sci 37:188–193
    [Google Scholar]
  15. Filip C., Fletcher G., Wulff J. L., Earhart C. F. 1973; Solubilization of the cytoplasmic membrane of Escherichia coll by the ionic detergent sodium lauryl sarcosinate. J. Bacteriol 115:717–722
    [Google Scholar]
  16. Frank G. H. 1989 Pasteurellosis of cattle. 197–222 Adlam C., Rutter M. J.ed Pasteurella and pasteurellosis Academic Press; London:
    [Google Scholar]
  17. Fraser J., Laird S., Gilmour N. J. L. 1982; A new serotype (biotype T) of Pasteurella haemolytica. Res. Vet. Sci 32:127–128
    [Google Scholar]
  18. Frederiksen W. 1973; Pasteurella taxonomy and nomenclature. Contrib. Microbiol. Immunol 2:170–176
    [Google Scholar]
  19. Gilmour N. J. L., Gilmour J. S. 1989 Pasteurellosis of sheep. 223–261 Adlam C., Rutter M. J.ed Pasteurella and pasteurellosis Academic Press; London:
    [Google Scholar]
  20. Hitchcock P. J., Brown T. M. 1983; Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol 154:269–277
    [Google Scholar]
  21. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism 3 Academic Press, Inc.; New York:
    [Google Scholar]
  22. Kusukawa N., Uemori T., Asada K., Kato I. 1990; Rapid and reliable protocol for direct sequencing of material amplified by the polymerase chain reaction. BioTechniques 9:66–72
    [Google Scholar]
  23. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
    [Google Scholar]
  24. Markwell M. A. K., Haas S. M., Bieber L. L., Tolbert N. E. 1978; A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem 87:206–210
    [Google Scholar]
  25. Mutters R., Bisgaard M., Pohl S. 1986; Taxonomic relationship of selected biogroups of Pasteurella haemolytica as revealed by DNArDNA hybridizations. Acta Pathol. MicrobioL Immunol. Scand. Sect. B 94:195–202
    [Google Scholar]
  26. Mutters R., Ihm P., Pohl S., Frederiksen W., Mannheim W. 1985; Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella cams, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa. Int. J. Syst. Bacteriol 35:309–322
    [Google Scholar]
  27. Mutters R., Mannheim W., Bisgaard M. 1989 Taxonomy of the group. 3–34 Adlam C., Rutter M. J.ed Pasteurella and pasteurellosis Academic Press; London:
    [Google Scholar]
  28. Paster B. J., Dewhirst F. E. 1988; Phylogeny of Campylobacters, wolinellas, Bacteroides gracilis, and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int. J. Syst. Bacteriol 38:56–62
    [Google Scholar]
  29. Quirie M., Donachie W., Gilmour N. J. L. 1986; Serotypes of Pasteurella haemolytica from cattle. Vet. Rec 119:93–94
    [Google Scholar]
  30. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4:406–425
    [Google Scholar]
  31. Sneath P. H. A., Stevens M. 1985; A numerical taxonomic study of Actinobacillus, Pasteurella and Yersinia. J. Gen. Microbiol 131:2711–2738
    [Google Scholar]
  32. Sneath P. H. A., Stevens M. 1990; Actinobacillus rossii sp. nov., Actinobacillus seminis sp. nov., nom. rev., Pasteurella bettii sp. nov., Pasteurella lymphangitidis sp. nov., Pasteurella main sp. nov., and Pasteurella trehalosi sp. nov. Int. J. Syst. Bacteriol 40:148–153
    [Google Scholar]
  33. Studier J., Keppler K. 1988; A note on the neighbor-joining algorithm of Saitou and Nei. Mol. Biol. Evol 5:729–731
    [Google Scholar]
  34. Tsai C. M., Frasch C. E. 1982; A sensitive silver stain for detecting lipopolysaccharide in polyacrylamide gels. Anal. Biochem 119:115–119
    [Google Scholar]
  35. Younan M., Fodor I. 1995; Characterization of a new Pasteurella haemolytica serotype (A17). Res. Vet. Sci 58:98
    [Google Scholar]
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