- International Journal of Systematic and Evolutionary Microbiology
- Volume 46, Issue 4
f Phylogenetic Positions of Clostridium chauvoei and Clostridium septicum Based on 16S rRNA Gene Sequences
- Authors: PETER KUHNERT*, SELJA E. CAPAUL, JACQUES NICOLET, JOACHIM FREY
- *Corresponding author. Mailing address: Institute of Veterinary Bacteriology, Laenggass-Str. 122, CH-3012 Bern. Switzerland. Phone: 41-31-6312369. Fax: 41-31-6312634. Electronic mail address: Kuhnert@vbi.unibe.ch.
- Int J Syst Evol Microbiol, October 1996 46: 1174-1176, doi: 10.1099/00207713-46-4-1174
- Subject: Original Papers Relating To Systematic Bacteriology
- Published Online:
The sequences of the 16S rRNA genes (rrs genes) of Clostridium chauvoei, the causative agent of blackleg in cattle, and the phenotypically related organism Clostridium septicum were determined. After amplification of 1,507-bp PCR fragments from the corresponding rrs genes, the sequences were determined in a single round of sequencing by using conserved region primers. A sequence similarity analysis of the sequences revealed the close phylogenetic relationship of C. chauvoei and C. septicum in Clostridium cluster I (M. D. Collins, P. A. Lawson, A. Willems, J. J. Cordoba, J. Fernandez-Garayzabal, P. Garcia, J. Cai, H. Hippe, and J. A. E. Farrow, Int. J. Syst. Bacteriol. 44:812-826. 1994), which includes Clostridium carnis, Clostridium perfringens, Clostridium botulinum, and Clostridium tetani. We found that 99.3% of the nucleotides in the genes of C. chauvoei and C. septicum are identical.
Copyright © 1996 International Union of Microbiological Societies | Published by the Microbiology Society
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