1887

Abstract

Hydrolysis of arginine is a classical diagnostic test for species in the mollicute order . In this paper we report data for arginine utilization by spiroplasmas, as determined by standard methods. In addition, modified methods were developed for fastidious spiroplasmas, such as strain LD-1 (T = type strain), the Colorado potato beetle spiroplasma. Twenty-one spiroplasma strains representing 13 groups or subgroups and eight ungrouped spiroplasmas (seven of which represent putative groups) were studied. The arginine reactions of eight strains were the same as the reactions reported previously, but previously reported positive tests for spiroplasma subgroups I-5 and I-6 () could not be repeated, and the data for the latter taxa are corrected. Although other workers have reported that addition of carbohydrate to media may be necessary for the utilization of arginine, the presence of glucose tended to obscure arginine hydrolysis in our studies.

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1996-10-01
2024-04-27
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References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for characterization of Mycoplasma species. Int. J. Syst. Bacteriol 20:35–58
    [Google Scholar]
  2. Barile M. 1983 Arginine hydrolysis. 345–349 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press, Inc.; New York:
    [Google Scholar]
  3. Barile M. F., Schmike R. T., Riggs D. B. 1966; Presence of arginine dihydrolase pathway in Mycoplasma. J. Bacteriol 91:189–192
    [Google Scholar]
  4. Hackett K. J., Lynn D. E. 1995 Insect cell culture approaches in cultivating spiroplasmas. 55–64 Razin S., Tully J. G.ed Molecular and diagnostic procedures in mycoplasmology 1 Academic Press, Inc.; San Diego, Calif:
    [Google Scholar]
  5. Hackett K. J., Whitcomb R. F. 1995 Cultivation of spiroplasmas in undefined and defined media. 41–54 Razin S., Tully J. G.ed Molecular and diagnostic procedures in mycoplasmology 1 Academic Press, Inc.; San Diego, Calif:
    [Google Scholar]
  6. Hackett K. J., Whitcomb R. F., Clark T. B., Henegar R. B., Lynn D. E., Wagner A. G., Tully J. G., Gasparich G. E., Rose D. L., Carle P., Bove J. M., Konai M., Clark E. A., Adams J. R., Williamson D. L. 1996; Spiroplasma leptinotarsae sp. nov., a new species from the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Int. J. Syst. Bacteriol 46:906–911
    [Google Scholar]
  7. Miles R. J. 1992; Catabolism in Mollicutes. J. Gen. Microbiol 138:1773–1783
    [Google Scholar]
  8. Olson L. D., Renshaw C. A., Rottem S., Boal J. H. 1993; Arginine utilization by Mycoplasma fermentans is not regulated by glucose metabolism: a 13C-NMR study. FEMS Microbiol. Lett 108:47–52
    [Google Scholar]
  9. Patterson-Curtis S. I., Johnson E. A. 1992; Roles of arginine in growth of Clostridium botulinum Okra B. Appl. Environ. Microbiol 58:2334–2337
    [Google Scholar]
  10. Reinsch C. H. 1967; Smoothing by spline functions. Numerische Math 10:177–183
    [Google Scholar]
  11. Saglio P., L’Hospital M., Lafleche D., Dupont G., Bove J. M., Tully J. G., Freundt E. A. 1973; Spiroplasma citri gen. and sp. n.: a mycoplasma-like organism associated with “stubborn” disease of citrus. Int. J. Syst. Bacteriol 23:191–204
    [Google Scholar]
  12. SAS Institute, Inc 1990 SAS graph software, version 6. , 1st. 1 SAS Institute, Inc.; Cary, N. C.:
    [Google Scholar]
  13. Shirazi I., Tarshis M., Rottem S. 1995; An arginine-ornithine exchange system in Spiroplasma melliferum cells. Microbiology 141:2323–2328
    [Google Scholar]
  14. Stevens C., Cody R. M., Gudauskas R. T. 1983; Effects of arginine on growth and in prolonging longevity of corn stunt spiroplasma in vitro. J. Gen. Appl. Microbiol 29:1–7
    [Google Scholar]
  15. Townsend R. 1976; Arginine metabolism by Spiroplasma citri. J. Gen. Microbiol 94:417–420
    [Google Scholar]
  16. Tully J. G., Bove J. M., Laigret F., Whitcomb R. F. 1993; Revised taxonomy of the class Mollicutes: proposed elevation of a major cluster of arthropod-associated mollicutes to ordinal rank (Entomoplasmatales ord. nov.), with provision for familial rank to separate species with nonhelical morphology (Entomoplasmataceae) from helical species (Spiroplasmataceae), and emended descriptions of the order Mycoplasmatales, family Mycoplasma taceae. Int. J. Syst. Bacteriol 43:378–385
    [Google Scholar]
  17. Tully J. G., Razin S.ed 1995 Molecular and diagnostic procedures in mycoplasmology. 2460–462 Academic Press, Inc.; San Diego, Calif:
    [Google Scholar]
  18. Tully J. G., Rose D. L., Clark E., Carle P., Bove J. M., Henegar R. B., Whitcomb R. F., Colflesh D. E., Williamson D. L. 1987; Revised group classification of the genus Spiroplasma (class Mollicutes), with proposed new groups XII to XXIII. Int. J. Syst. Bacteriol 37:357–364
    [Google Scholar]
  19. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Van Etten J., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J. Bacteriol 171:6455–6467
    [Google Scholar]
  20. Whitcomb R. F., Hackett K. J. 1995 Identification of mollicutes from insects. 313–322 Tully J. G., Razin S.ed Molecular and diagnostic procedures in mycoplasmology 2 Academic Press, Inc.; San Diego, Calif:
    [Google Scholar]
  21. Williamson D. L. 1989 The genus Spiroplasma. 71–111 Whitcomb R. F., Tully J. G.ed The mycoplasmas 5 Academic Press, Inc.; New York:
    [Google Scholar]
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