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Volume 47, Issue 1

sp. nov., from the Southern Corn Rootworm Beetle, (Coleoptera: Chrysomelidae) Free

Abstract

Abstract

Spiroplasma strain DU-1 (T = type strain), which was isolated from hemolymph of the corn rootworm (Coleoptera: Chrysomelidae), was serologically distinct from other spiroplasma species, groups, and subgroups. Cells of strain DU-1 were shown by light microscopy to be helical motile filaments. Electron microscopy revealed cells bounded by a single cytoplasmic membrane, with no evidence of a cell wall. The organism was not sensitive to 500 U of penicillin per ml. Strain DU-1 grew well in SM-1, M1D, and SP-4 liquid media, in broth supplemented with 1% bovine serum fraction or conventional horse serum, and under both aerobic and anaerobic conditions. This organism did not appear to have a sterol requirement for growth, as has been reported for several other species or strains. Optimal growth occurred at 32°C, with a doubling time of 0.9 h; strain DU-1 multiplied at 10 to 41°C but failed to grow at 5 or 43°C. It produced acid from glucose but hydrolyzed neither arginine nor urea. The results of reciprocal serologic tests in which antigens or antisera to established species, groups, subgroups, and putative groups were used indicated that strain DU-1 was serologically distinct. This organism has a DNA guanine-plus-cytosine content of 25 ± 1 mol% and a genome size of 1,350 kbp. Strain DU-1 is a member of a cluster of fast-growing insect-associated spiroplasmas, as determined by sequence analysis of 16S rRNA. On the basis of the results of this study and previously published data, strain DU-1 (= ATCC 43210) is designated the type strain of a new species, .

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Copyright 1997 International Union of Microbiological Societies
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1997-01-01
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Spiroplasma diabroticae sp. nov., from the Southern Corn Rootworm Beetle, Diabrotica undecimpunctata (Coleoptera: Chrysomelidae)
Int J Syst Evol Microbiol 47, 78 (1997); https://doi.org/10.1099/00207713-47-1-78
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