- International Journal of Systematic and Evolutionary Microbiology
- Volume 47, Issue 2
f Phylogeny of Thiobacillus cuprinus and Other Mixotrophic Thiobacilli: Proposal for Thiomonas gen. nov.
- Authors: DAVID MOREIRA, RICARDO AMILS*
- * Corresponding author. Mailing address: Centro de Biología Molecular “Severo Ochoa,” Lab. C-101, Universidad AutÓnoma de Madrid, Cantoblanco, 28049 Madrid, Spain. Phone: (34) 1 397 8077. Fax: (34) 1 397 8087. E-mail: firstname.lastname@example.org.
- First Published Online: 01 April 1997, International Journal of Systematic and Evolutionary Microbiology 47: 522-528, doi: 10.1099/00207713-47-2-522
- Subject: Original Papers Relating To Systematic Bacteriology
- Issue Published:
The complete 5S and 16S ribosomal DNA (rDNA) sequences of the facultatively chemolithotrophic bacterium Thiobacillus cuprinus and results of a comparison of these sequences with homologous sequences from several proteobacterial species supported affiliation of T. cuprinus with the β subgroup of the Proteobacteria. T. cuprinus, Thiobacillus intermedius, Thiobacillus perometabolis, and Thiobacillus thermosulfatus form a phylogenetic cluster that comprises some of the thiobacilli capable of mixotrophic growth. This cluster is related to some pseudomonads and Alcaligenes species belonging to the β subclass. In addition, a low-frequency restriction fragment analysis (LFRFA) of some mixotrophic thiobacilli and some related species was carried out by using pulsed-field gel electrophoresis (PFGE) to determine the Spe I and Xba I macrorestriction patterns and genome sizes of these organisms. The correlation of the LFRFA results and the 16S rDNA analysis results and the usefulness of the two analyses are discussed. The PFGE fingerprints suggested that Thiobacillus sp. strain ATCC 27793 is related to T. intermedius rather than to T. perometabolis, as described previously. The distinctive characteristics of the mixotrophic species analyzed in this work, their phylogenetic relatedness, and their physiological differences from other groups belonging to the Proteobacteria, including other thiobacilli, suggest that these organisms should be transferred to a new genus, the genus Thiomonas gen. nov.
Copyright © 1997 International Union of Microbiological Societies | Published by the Microbiology Society
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