- International Journal of Systematic and Evolutionary Microbiology
- Volume 47, Issue 2
f Thermoterrabacterium ferrireducens gen. nov., sp. nov., a Thermophilic Anaerobic Dissimilatory Fe(III)-Reducing Bacterium from a Continental Hot Spring
- Authors: ALEXANDER SLOBODKIN, ANNA-LOUISE REYSENBACH, NATHALIE STRUTZ, MICHAEL DREIER, JUERGEN WIEGEL*
- * Corresponding author. Mailing address: Department of Microbiology, 215 Biological Sciences Building, University of Georgia, Athens, GA 30602-2605. Phone: (706) 542-2651. Fax: (706) 542-2674. Electronic mail address: firstname.lastname@example.org.
- Int J Syst Evol Microbiol, April 1997 47: 541-547, doi: 10.1099/00207713-47-2-541
- Subject: Original Papers Relating To Systematic Bacteriology
- Published Online:
A strain of a thermophilic, anaerobic, dissimilatory, Fe(III)-reducing bacterium, Thermoterrabacterium ferrireducens gen. nov., sp. nov. (type strain JW/AS-Y7T; DSM 11255), was isolated from hot springs in Yellowstone National Park and New Zealand. The gram-positive-staining cells occurred singly or in pairs as straight to slightly curved rods, 0.3 to 0.4 by 1.6 to 2.7 μm, with rounded ends and exhibited a tumbling motility. Spores were not observed. The temperature range for growth was 50 to 74°C with an optimum at 65°C. The pH range for growth at 65°C was from 5.5 to 7.6, with an optimum at 6.0 to 6.2. The organism coupled the oxidation of glycerol to reduction of amorphous Fe(III) oxide or Fe(III) citrate as an electron acceptor. In the presence as well as in the absence of Fe(III) and in the presence of CO2, glycerol was metabolized by incomplete oxidation to acetate as the only organic metabolic product; no H2 was produced during growth. The organism utilized glycerol, lactate, 1,2-propanediol, glycerate, pyruvate, glucose, fructose, mannose, and yeast extract as substrates. In the presence of Fe(III) the bacterium utilized molecular hydrogen. The organism reduced 9,10-anthraquinone-2,6-disulfonic acid, fumarate (to succinate), and thiosulfate (to elemental sulfur) but did not reduce MnO2, nitrate, sulfate, sulfite, or elemental sulfur. The G+C content of the DNA was 41 mol% (as determined by high-performance liquid chromatography). The 16S ribosomal DNA sequence analysis placed the isolated strain as a member of a new genus within the gram-type-positive Bacillus-Clostridium subphylum.
Copyright © 1997 International Union of Microbiological Societies | Published by the Microbiology Society
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