RT Journal Article SR Electronic(1) A1 Liu, Yitai A1 Karnauchow, Tim M. A1 Jarrell, Ken F. A1 Balkwill, David L. A1 Drake, Gwendolyn R. A1 Ringelberg, David A1 Clarno, Ronald A1 Boone, David R.YR 1997 T1 Description of Two New Thermophilic Desulfotomaculum spp., Desulfotomaculum putei sp. nov., from a Deep Terrestrial Subsurface, and Desulfotomaculum luciae sp. nov., from a Hot Spring JF International Journal of Systematic and Evolutionary Microbiology, VO 47 IS 3 SP 615 OP 621 DO https://doi.org/10.1099/00207713-47-3-615 PB Microbiology Society, SN 1466-5034, AB Abstract Six strains of thermophilic, endospore-forming, sulfate-reducing bacteria were enriched and isolated from 2.7 km below the earth’s surface in the Taylorsville Triassic Basin in Virginia. The cells of these strains were motile rods that were 1 to 1.1 µm in diameter and 2 to 5 µm long. The cells grew by oxidizing H2, formate, methanol (weakly), lactate (incompletely, to acetate and CO2), or pyruvate (incompletely) while reducing sulfate to sulfide; acetate did not serve as a catabolic substrate. Thiosulfate or sulfite could replace sulfate as an electron acceptor. The results of a phylogenetic analysis of the 16S rRNA gene indicated that these strains belong to the genus Desulfotomaculum, but are distinct from previously described Desulfotomaculum species. Thus, we propose a new species, Desulfotomaculum putei, for them, with strain TH-11 (= SMCC W459) as the type strain. The results of our phylogenetic analysis also indicated that strain SLTT, which was isolated from a hot spring and has been described previously (T. M. Karnauchow, S. F. Koval, and K. F. Jarrell, Syst. Appl. Microbiol. 15:296–310, 1992), is also a member of the genus Desulfotomaculum and is distinct from other species in this genus. We therefore propose the new species Desulfotomaculum luciae for this organism; strain SLT (= SMCC W644) is the type strain of D. luciae., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-47-3-615