1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 47, Issue 3

sp. nov., a Urease-Negative Species Isolated from Laboratory Mice Free

Abstract

Abstract

A spiral-shaped bacterium with bipolar, single, nonsheathed flagella was isolated from the intestines of laboratory mice. The organism grew at 37 and 42°C under microaerobic and anaerobic conditions, did not hydrolyze urea, was weakly positive for catalase and oxidase, reduced nitrate to nitrite, did not hydrolyze indoxyl acetate or hippurate, and was resistant to cephalothin and nalidixic acid. This is the first urease-negative, murine spp. isolated from intestines. Also, and this bacterium are unique among the genus in having nonsheathed flagella. The new bacterium appears to be part of the normal intestinal flora; although its pathogenic potential is unknown, this organism was also isolated from mice with diarrhea that were co-infected with On the basis of 16S rRNA gene sequence analysis data and biochemical and phenotypic criteria, the new organism is classified as a novel helicobacter, for which we propose the name The type strain is MIT 95-1707 (= ATCC 700285).

  • Published Online:
© Society for General Microbiology, 1997
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-3-627
1997-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/3/ijs-47-3-627.html?itemId=/content/journal/ijsem/10.1099/00207713-47-3-627&mimeType=html&fmt=ahah

References

  1. Archer J. R., Romero S., Ritchie A. E. 1988; Characterization of an unclassified microaerophilic bacterium associated with gastroenteritis. J. Clin. Microbiol. 26:101–105
     [Google Scholar]
  2. Bode G., Mauch F., Ditschuneit H., Malfertheiner P. 1993; Identification of structures containing polyphosphate in Helicobacter pylori. J. Gen. Microbiol. 139:3029–3033
     [Google Scholar]
  3. Eaton K. A., Dewhirst F. E., Radin M. J. 1993; Helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. Int. J. Syst. Bacteriol. 43:99–106
     [Google Scholar]
  4. Forman D., Newell D. G., Fullerton F. 1991; Association between infection with Helicobacter pylori and risk of gastric cancer: evidence from a prospective investigation. Br. Med. 302:1302–1305
     [Google Scholar]
  5. Fox J., Shomer N. Unpublished data
     [Google Scholar]
  6. Fox J. G., Blanco M., Murphy J. C., Taylor N. S., Lee A., Kabok Z., Pappo J. 1993; Local and systemic immune response in murine Helicobacter felis active chronic gastritis. Infect. Immun. 61:2309–2315
     [Google Scholar]
  7. Fox J. G., Correa P., Taylor N. S., Lee A., Otto G., Murphy J. C., Rose R. 1990; Helicobacter mustelae associated gastritis in ferrets: an animal model of Helicobacter pylori gastritis in humans. Gastroenterology 99:352–361
     [Google Scholar]
  8. Fox J. G., Dewhirst F. E., Tully J. G., Paster B. J., Yan L., Taylor N. S., Collins M. J., Gorelick P. L., Ward J. M. 1994; Helicobacter hepaticus sp. nov., a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice. J. Clin. Microbiol. 32:1238–1245
     [Google Scholar]
  9. Fox J. G., Lee A. 1993; Gastric helicobacter infection in animals: natural and experimental infections. 407–430 Goodwin C. S., Worsley B. W. Biology and clinical practice CRC Press; Boca Raton, Fla.:
     [Google Scholar]
  10. Fox J. G., Li X., Yan L., Cahill R. J., Hurley R., Lewis R., Murphy J. C. 1996; Chronic proliferative hepatitis in A/JCr mice associated with persistent Helicobacter hepaticus infection: a model of helicobacter-induced carcinogenesis. Infect. Immun. 64:1548–1558
     [Google Scholar]
  11. Fox J. G., Yan L., Dewhirst F. E., Paster B. J., Shames B., Murphy J. C., Hayward A., Belcher J. C., Mendes E. N. 1995; Helicobacter bilis sp. nov., a novel helicobacter isolated from bile, livers, and intestines of aged, inbred mouse strains. J. Clin. Microbiol. 33:445–454
     [Google Scholar]
  12. Fox J. G., Yan L., Shames B., Campbell J., Murphy J. C., Li X. 1996; Persistent hepatitis and enterocolitis in germfree mice infected with Helicobacter hepaticus. Infect. Immun. 64:3673–3681
     [Google Scholar]
  13. Gebhart C. J., Fennell C. L., Murtaugh M. P., Stamm W. E. 1989; Campylobacter cinaedi is normal intestinal flora in hamsters. J. Clin. Microbiol. 27:1692–1694
     [Google Scholar]
  14. Hanninen M. L., Happonen I., Saari S. 1996; Culture and characteristics of Helicobacter bizzozeronii, a new canine gastric Helicobacter sp. Int. J. Syst. Bacteriol. 46:160–166
     [Google Scholar]
  15. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. 21–132 Munro H. N. Mammalian protein metabolism Academic Press; New York, N.Y.:
     [Google Scholar]
  16. Lee A., Philips M. W., O’Rourke J. L., Paster B. J., Dewhirst F. E., Fraser G. J., Fox J. G., Sly L. I., Romaniuk P. J., Trust T. J., Kouprach S. 1992; Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. Int. J. Syst. Bacteriol. 42:27–36
     [Google Scholar]
  17. Mendes E. N., Queiroz D. M. M., Dewhirst F. E., Paster B. J., Moura S. B., Fox J. G. 1996; Helicobacter trogontum sp. nov., isolated from the rat intestine. Int. J. Syst. Bacteriol. 46:916–921
     [Google Scholar]
  18. Nomura A., Stenimerman G. N., Chyou P. H., Kato I., Perez-Perez G. I., Blaser M. J. 1991; Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii. N. Engl. J. Med. 325:1132–1136
     [Google Scholar]
  19. Parsonnet J., Friedman G. D., Vandersteen D. P., Chang Y., Vogelman J. H., Orentreich N., Sibley R. K. 1991; Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 325:1127–1131
     [Google Scholar]
  20. Paster B. J., Dewhirst F. E. 1988; Phylogeny of Campylobacter, wolinellas, Bacteroides gracilis, and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int. J. Syst. Bacteriol. 38:56–62
     [Google Scholar]
  21. Queiroz D. M. M., Contigli C., Coimbra R. S. 1992; Spiral bacterium associated with gastric, ileal and caecal mucosa of mice. Lab. Anim. 26:288–294
     [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
     [Google Scholar]
  23. Schauer D. B., Ghori N., Falkow S. 1993; Isolation and characterization of “Flexispira rappini” from laboratory mice. J. Clin. Microbiol. 31:2709–2714
     [Google Scholar]
  24. Shames B., Fox J. G., Dewhirst F. E., Yan L., Shen Z., Taylor N. S. 1995; Identification of widespread Helicobacter hepaticus infection in feces in commercial mouse colonies by culture and PCR assay. J. Clin. Microbiol. 33:2968–2972
     [Google Scholar]
  25. Stanley J., Linton D., Bürens A. P. 1994; Helicobacter pullorum sp. nov.—genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis. Microbiology 140:3441–3449
     [Google Scholar]
  26. Totten P. A., Fennel C. L., Tenover F. C. 1985; Campylobacter cinaedi (sp. nov.) and Campylobacter fennelliae (sp. nov.): two new Campylobacter species associated with enteric disease in homosexual men. J. Infect. Dis. 151:131–139
     [Google Scholar]
  27. Ward J. M., Anver M. R., Haines D. C., Melhorn J. M., Gorelick P., Yan L., Fox J. G. 1996; Inflammatory large bowel disease in immunodeficient mice naturally infected with Helicobacter hepaticus. Lab. Anim. Sci. 46:15–20
     [Google Scholar]
  28. Ward J. M., Fox J. G., Anver M. R., Haines D. C., George C. V., Collins M. J., Gorelick P. L., Nagashima K., Gonda M. A., Gilden R. V., Tully J. G., Russell R. J., Benveniste R. E., Paster B. J., Dewhirst F. E., Donovan J. C., Anderson L. M., Rice J. M. 1994; Chronic active hepatitis and associated liver tumors in mice caused by a persistent bacterial infection with a novel Helicobacter species. J. Natl. Cancer Inst. 86:1222–1227
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-3-627
Loading
Helicobacter rodentium sp. nov., a Urease-Negative Helicobacter Species Isolated from Laboratory Mice
Int J Syst Evol Microbiol 47, 627 (1997); https://doi.org/10.1099/00207713-47-3-627
/content/journal/ijsem/10.1099/00207713-47-3-627
/content/journal/ijsem/10.1099/00207713-47-3-627
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error