1887

Abstract

Ten strains of a hitherto undescribed catalase-negative, facultatively anaerobic, coryneform bacterium were isolated or collected by workers at three European clinical bacteriology laboratories or reference centers. These strains were isolated from humans, and most came from abscess material. Biochemical and chemotaxonomic characterization revealed that the strains belonged to the genus . The phenotypic features of the 10 strains were incompatible with the descriptions of the previously established species. A comparative 16S rRNA gene sequence analysis demonstrated that the previously undescribed strains constitute a new line in the genus . The name sp. nov. is proposed for these clinical isolates. The type strain is CCUG 32789A.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-3-687
1997-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/3/ijs-47-3-687.html?itemId=/content/journal/ijsem/10.1099/00207713-47-3-687&mimeType=html&fmt=ahah

References

  1. Bernard K. A., Bellefeuille M., Ewan E. P. 1991; Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods. J. Clin. Microbiol. 29:83–89
    [Google Scholar]
  2. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387–395
    [Google Scholar]
  3. Felsenstein J. 1989; PHYLIP—phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  4. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284
    [Google Scholar]
  5. Funke G., Martinetti-Lucchini G., Pfyffer G. E., Marchiani M., von Graevenitz A. 1993; Characteristics of CDC group 1 and group 1-like coryneform bacteria isolated from clinical specimens. J. Clin. Microbiol. 31:2907–2912
    [Google Scholar]
  6. Funke G., Pascual Ramos C., Collins M. D. 1995; Identification of some clinical strains of CDC coryneform group A-3 and group A-4 bacteria as Cellulomonas species and proposal of Cellulomonas hominis sp. nov. for some group A-3 strains. J. Clin. Microbiol. 33:2091–2097
    [Google Scholar]
  7. Funke G., Pascual Ramos C., Fernandez-Garayzabal J., Weiss N., Collins M. D. 1995; Description of human-derived Centers for Disease Control coryneform group 2 bacteria as Actinomyces bemardiae sp. nov. Int. J. Syst. Bacteriol. 45:57–60
    [Google Scholar]
  8. Funke G., von Graevenitz A., Clarridge J. E. III, Bernard K. A. 1997; Clinical microbiology of coryneform bacteria. Clin. Microbiol. Rev. 10:125–159
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C.ed 1977 Anaerobe laboratory manual, 4th. Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  10. Hollis D. G., Weaver R. E. 1981 Gram-positive organisms: a guide to identification Special Bacteriology Section, Centers for Disease Control; Atlanta, Ga.:
    [Google Scholar]
  11. Hutson R. A., Thompson D. E., Collins M. D. 1993; Genetic interrelationships of saccharolytic Clostridium botulinum types B, E and F and related Clostridia as revealed by small-subunit rRNA gene sequences. FEMS Microbiol. Lett. 108:103–110
    [Google Scholar]
  12. Johnson J. L., Moore L. V. H., Kaneko B., Moore W. E. C. 1990; Actinomyces georgiae sp. nov., Actinomyces gerencseriae sp. nov., designation of two genospecies of Actinomyces naeslundii, and inclusion of A. naeslundii serotypes II and III and Actinomyces viscosus serotype II in A. naeslundii genospecies 2. Int. J. Syst. Bacteriol. 40:273–286
    [Google Scholar]
  13. Lindquist D., Wong J. 1993; ActinomycespyogenesAike bacteria. J. Clin. Microbiol. 31:3353–3354 Letter to the editor
    [Google Scholar]
  14. Pascual Ramos C., Foster G., Collins M. D. 1997; Phylogenetic analysis of the genus Actinomyces based on 16S rRNA gene sequences: description of Arcanobacterium phocae sp. nov., Arcanobacterium bemardiae comb, nov., and Arcanobacterium pyogenes comb. nov. Int. J. Syst. Bacteriol. 47:46–53
    [Google Scholar]
  15. Pot B., Vandamme P., Kersters K. 1994 Analysis of electrophoretic whole-organism protein fingerprints. 493–521 Goodfellow M., O’Donnell A. G.ed Modern microbial methods. Chemical methods in prokaryotic systematics J. Wiley and Sons Ltd.; Chichester, United Kingdom:
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  17. Schaal K. P. 1986 Genus Actinomyces. 1383–1418 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore, Md:
    [Google Scholar]
  18. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36:407–477
    [Google Scholar]
  19. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44:846–849
    [Google Scholar]
  20. von Graevenitz A., Funke G. 1996; An identification scheme for rapidly and aerobically growing gram-positive rods. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. 284:246–254
    [Google Scholar]
  21. von Graevenitz A., Osterhaut G., Dick J. 1991; Grouping of some clinically relevant gram-positive rods by automated fatty acid analysis. APMIS 99:147–154
    [Google Scholar]
  22. Weiss N. Unpublished data
  23. Wiist J., Martinetti Lucchini G., Liithy-Hottenstein J., Brun F., Altwegg M. 1993; Isolation of gram-positive rods that resemble but are clearly distinct from Actinomyces pyogenes from mixed wound infections. J. Clin. Microbiol. 31:1127–1135
    [Google Scholar]
  24. Wiist J., Stubbs S., Weiss N., Funke G., Collins M. D. 1995; Assignment of Actinomyces pyogenesAike (CDC coryneform group E) bacteria to the genus Actinomyces as Actinomyces radingae sp. nov. and Actinomyces turicensis sp. nov. Lett. Appl. Microbiol. 20:76–81
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-3-687
Loading
/content/journal/ijsem/10.1099/00207713-47-3-687
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error