1887

Abstract

Spiroplasma strain PUP-1was isolated from the gut fluids of a firefly beetle () collected in Maryland. Cells of the strain were shown by dark-field microscopy to be helical, motile filaments. Ultrastructural examination by electron microscopy revealed filamentous cells bounded by a single cytoplasmic membrane and no evidence of a cell wall. The cells were not sensitive to 500 U of penicillin per ml and grew under aerobic conditions in M1D, SP-4, and M-2 broth formulations, as well as in conventional mycoplasma medium. The doubling times at 15, 20, 25, and 30°C were 83.1, 32.0, 14.9, and 9.8 h, respectively. Suboptimal growth occurred at 37°C, and no growth was apparent in cultures maintained at 10 or 40°C. The organism required cholesterol for growth and produced acid from glucose, fructose, and trehalose; arginine and urea were not hydrolyzed. The results of previous serological analyses of strain PUP-1indicated that the organism was not related to the then currently established species or group representatives, and the organism was classified as the representative of group XIX. Additional testing of strain PUP-1against recently recognized species or group representatives by both metabolism inhibition and deformation tests confirmed the unique serological status of the organism. The guanine-plus-cytosine content of the DNA was 26 ± 1 mol%, and the genome size was 1,375 kbp. These values clearly differentiate strain PUP-1from group XXI strain W115, with which it cross-reacted reciprocally at a low level in deformation and metabolism inhibition tests. We propose that strain PUP-1 (= ATCC 43206) should be recognized as the type strain of a new species, .

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1997-07-01
2024-03-28
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References

  1. Abalain-Colloc M. L., Chastel C., Tully J. G., Bove J. M., Whitcomb R. F., Gilot B., Williamson D. L. 1987; Spiroplasma sabaudiense sp. nov. from mosquitoes collected in France. Int. J. Syst. Bacteriol. 37:260–265
    [Google Scholar]
  2. Adams J. R., Whitcomb R. F., Tully J. G., Clark E. A., Rose D. L., Carle P., Konai M., Bove J. M., Henegar R. B., Williamson D. L. 1997; Spiroplasma alleghenese sp. nov., a new species from the scorpion fly Planorpa helena (Mecoptera: Planorpidae). Int. J. Syst. Bacteriol. 47:759–762
    [Google Scholar]
  3. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
    [Google Scholar]
  4. Carle P., Laigret F., Tully J. G., Bove J. M. 1995; Heterogeneity of genome sizes within the genus Spiroplasma. Int. J. Syst. Bacteriol. 45:178–181
    [Google Scholar]
  5. Chastel C., Humphrey-Smith I. 1991; Mosquito spiroplasmas. Adv. Dis. Vector Res. 7:149–205
    [Google Scholar]
  6. Clark T. B., Henegar R. B., Rosen L., Hackett K. J., Whitcomb R. F., Lowry J. E., Saillard C., Bové J. M., Tully J. G., Williamson D. L. 1987; New spiroplasmas from insects and flowers: isolation, ecology, and host association. Isr. J. Med. Sci. 23:687–690
    [Google Scholar]
  7. Clark T. B., Whitcomb R. F., Tully J. G., Mouches C., Saillard C., Bové J. M., Wroblewski H., Carle P., Rose D. L., Henegar R. B., Williamson D. L. 1985; Spiroplasma melliferum, a new species from the honeybee (Apis mellifera). Int. J. Syst. Bacteriol. 35:296–308
    [Google Scholar]
  8. Hackett K. J., Clark T. B. 1989 Ecology of spiroplasmas. 113–200 Whitcomb R. F., Tully J. G.ed The mycoplasmas 5 Academic Press; San Diego, Calif:
    [Google Scholar]
  9. Hackett K. J., Whitcomb R. F., Clark T. B., Henegar R. B., Lynn D. E., Wagner A. G., Tully J. G., Gasparich G. E., Rose D. L., Carle P., Bové J. M., Konai M., Clark E. A., Adams J. R., Williamson D. L. 1996; Spiroplasma leptinotarsae sp. nov., a mollicute uniquely adapted to its host, the Colorado potato beetle, Leptinotarsa decemlineata (Coleóptera: Chrysomelidae). Int. J. Syst. Bacteriol. 46:906–911
    [Google Scholar]
  10. Hackett K. J., Whitcomb R. F., Tully J. G., Lloyd J. E., Anderson J. J., Clark T. B., Henegar R. B., Rose D. L., Clark E. A., Vaughn J. L. 1992; Lampyridae (Coleóptera): a plethora of mollicute associations. Microb. Ecol. 23:181–193
    [Google Scholar]
  11. Hung S. H. Y., Chen T. A., Whitcomb R. F., Tully J. G., Chen Y. X. 1987; Spiroplasma culicicola sp. nov. from the salt marsh mosquito Aedes sollicitans. Int. J. Syst. Bacteriol. 37:365–370
    [Google Scholar]
  12. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Moflientes 1995; Revised minimum standards for description of new species of the class Mollicutes (division Tenericutes). Int. J. Syst. Bacteriol. 45:605–612
    [Google Scholar]
  13. Jones A. L., Whitcomb R. F., Williamson D. L., Coan M. E. 1977; Comparative growth and primary isolation of spiroplasmas in media based on insect tissue culture formulations. Phytopathology 67:738–746
    [Google Scholar]
  14. Konai M., Clark E. A., Camp M., Koch A. L., Whitcomb R. F. 1996; Temperature ranges, growth optima, and growth rates of Spiroplasma (Spiroplasmataceae, class Mollicutes) species. Curr. Microbiol. 32:1–7
    [Google Scholar]
  15. Liao C. H., Chen T. A. 1977; Culture of corn stunt spiroplasma in a simple medium. Phytopathology 67:802–807
    [Google Scholar]
  16. Markham P. G., Clark T. B., Whitcomb R. F. 1983; Culture techniques for spiroplasmas from arthropods. Methods Mycoplasmol. 2:217–223
    [Google Scholar]
  17. Senterfit L. B. 1983; Preparation of antigens and antisera. Methods Mycoplasmol. 1:401–404
    [Google Scholar]
  18. Shaikh A. A., Johnson W. E. Jr., Stevens C., Tang A. Y. 1987; The isolation of spiroplasmas from mosquitoes in Macon Country, Alabama. J. Am. Mosq. Control Assoc. 3:289–295
    [Google Scholar]
  19. Tully J. G., Bové J. M., Laigret F., Whitcomb R. F. 1993; Revised taxonomy of the class Mollicutes: proposed elevation of a monophyletic cluster of arthropod-associated mollicutes to ordinal rank (Entomoplasmatales ord. nov.), with provision for familial rank to separate species with nonhelical morphology (Entomoplasmataceae fam. nov.) from helical species (Spiroplasmataceae), and emended descriptions of the order Mycoplasmatales, family Mycoplasmataceae. Int. J. Syst. Bacteriol. 43:378–385
    [Google Scholar]
  20. Tully J. G., Rose D. L., Clark E., Carle P., Bove J. M., Henegar R. B., Whitcomb R. F., Colflesh D. E., Williamson D. L. 1987; Revised group classification of the genus Spiroplasma (class Mollicutes), with proposed new groups XII to XXIII. Int. J. Syst. Bacteriol. 37:357–364
    [Google Scholar]
  21. Tully J. G., Razin S.ed 1996 Molecular and diagnostic procedures in mycoplasmology, vol. 2. 460–462 Academic Press; San Diego, Calif.:
    [Google Scholar]
  22. Tully J. G., Whitcomb R. F. 1991 The genus Spiroplasma. 1960–1980 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K. H.ed The prokaryotes, 2nd. 2 Springer-Verlag; New York, N.Y.:
    [Google Scholar]
  23. Wedincamp J. Jr. 1994; Laboratory infection and release of Spiroplasma (Entomoplasmatales: Spiroplasmataceae) from horse flies (Tabanidae: Diptera). M.S. thesis Georgia Southern University; Statesboro:
    [Google Scholar]
  24. Whitcomb R. F. 1983; Culture media for spiroplasmas. Methods Mycoplasmol. 1:147–158
    [Google Scholar]
  25. Whitcomb R. F., Bove J. M., Chen T. A., Tully J. G., Williamson D. L. 1987; Proposed criteria for an interim serogroup classification for members of the genus Spiroplasma (class Mollicutes). Int. J. Syst. Bacteriol. 37:82–84
    [Google Scholar]
  26. Whitcomb R. F., Tully J. G. 1984 The family Spiroplasmataceae, genus Spiroplasma. 781–787 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 Williams and Wilkins; Baltimore, Md:
    [Google Scholar]
  27. Whitcomb R. F., Tully J. G., Clark T. B., Williamson D. L., Bove J. M. 1982; Revised serological classification of spiroplasmas, new provisional groups, and recommendations for serotyping of isolates. Curr. Microbiol. 7:291–296
    [Google Scholar]
  28. Whitcomb R. F., French F. E., Tully J. G., Gasparich G. E., Rose D. L., Carle P., Bove J. M., Henegar R. B., Konai M., Hackett K. J., Adams J. R., Clark T. B., Williamson D. L. 1997; Spiroplasma chrysopicola sp. nov., Spiroplasma gladiatoris sp. nov., Spiroplasma helicoides sp. nov., and Spiroplasma tabanidicola sp. nov., from tabanid (Diptera: Tabanidae) flies. Int. J. Syst. Bacteriol. 47:713–719
    [Google Scholar]
  29. Williamson D. L., Whitcomb R. F., Tully J. G. 1978; The spiroplasma deformation test, a new serological method. Curr. Microbiol. 1:203–207
    [Google Scholar]
  30. Williamson D. L., Tully J. G., Whitcomb R. F. 1979; Serological relationships of spiroplasmas as shown by combined deformation and metabolism inhibition tests. Int. J. Syst. Bacteriol. 29:345–351
    [Google Scholar]
  31. Williamson D. L., Tully J. G., Whitcomb R. F. 1989 The genus Spiroplasma. 71–111 Whitcomb R. F., Tully J. G.ed The mycoplasmas 5 Academic Press; San Diego, Calif:
    [Google Scholar]
  32. Williamson D. L., Tully J. G., Rosen L., Rose D. L., Whitcomb R. F., Abalain-Colloc M.-L., Carle P., Bove J. M., Smyth J. 1996; Spiroplasma diminutum sp. nov., from Culex annulus mosquitoes collected in Taiwan. Int. J. Syst. Bacteriol. 46:229–233
    [Google Scholar]
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