1887

Abstract

On the basis of DNA-DNA hybridization data, nine intestinal spirochete strains were grouped into five genospecies. Three of these genospecies were previously recognized species, (type strain, B78), (type strain, B256), and (type strain, P43/6/78; previously “”). The other two genospecies were found to be new species, for which we propose the names sp. nov. (with type strain PWS/A) and sp. nov. (with type strain 56–150). and cells had a typical spirochete ultrastructure with 22 to 28 periplasmic flagella per cell. Various soluble sugars were growth substrates for and . During growth in basal heart infusion broth supplemented with fetal calf serum beneath an O-N (1:99) atmosphere, cells of these new species consumed oxygen and glucose and produced H, CO, acetate, butyrate, and ethanol. The G+C content of the DNA of 56–150 was 27 mol%, and the G+C content of the DNA of PWS/A was 25 mol%. In addition, a restriction fragment length polymorphism-PCR assay for the detection of intestinal spirochetes was developed. The assay was based on generation and restriction endonuclease analysis (with 3A, and II) of a 558-bp amplicon of ribosomal DNA (rDNA) encoding 16S rRNA. The PCR amplification was specific for species and . Four restriction digest patterns were found for the five species. fl. restriction differentiated and from the other species. 3A and I restrictions gave unique fragment patterns for and , respectively. and DNAs gave the same fragment pattern regardless of the enzyme tested. was differentiated from the species by II digestion of the 16S rDNA amplicon.

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1997-10-01
2024-03-28
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References

  1. Barrett S. P. 1990; Intestinal spirochaetes in a Gulf Arab population. Epidemiol. Infect. 104:261–266
    [Google Scholar]
  2. Binek M., Szynkiewicz Z. M. 1984; Physiological properties and classification of strains of Treponema sp. isolated from pigs in Poland. Comp. Immunol. Microbiol. Infect. Dis. 7:141–148
    [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc. Natl. Acad. Sci. USA 75:4801–4805
    [Google Scholar]
  4. Çrôsa J. H., Brenner D. J., Falkow S. 1973; Use of a single-strandspecific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacteriol. 115:904–911
    [Google Scholar]
  5. Cwyk W. M., Canale-Parola E. 1979; Treponema succinifaciens sp. nov., an anaerobic spirochete from the swine intestine. Arch. Microbiol. 122:231–239
    [Google Scholar]
  6. Elder R. O., Duhamel G. E., Schafer R. W., Mathiesen M. R., Ramanathan M. 1994; Rapid detection of Serpulina hyodysenteriae in diagnostic specimens by PCR. J. Clin. Microbiol. 32:1497–1502
    [Google Scholar]
  7. Fellstrôm C., Gunnarsson A. 1995; Phenotypic characterization of intestinal spirochaetes isolated from pigs. Res. Vet. Sci. 59:1–4
    [Google Scholar]
  8. Fellstrôm C., Pettersson B., Uhlen M., Gunnarsson A., Johansson K. E. 1995; Phylogeny of Serpulina based on sequence analyses of the 16S rRNA gene and comparison with a scheme involving biochemical classification. Res. Vet. Sci. 59:5–9
    [Google Scholar]
  9. Fournié-Amazouz E., Baranton G., Carlier J. P., Chambreuil G., Cohadon F., Collin P., Gougeon Jolivet A, Hermès I., Lemarie C., Saint Girons I. 1995; Isolations pf intestinal spirochaetes from the blood of human patients. J. Hosp. Infect. 30:160–162
    [Google Scholar]
  10. Grimont P. A. D., Popoff M. Y., Grimont F., Coynault C., Lemelin M. 1980; Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures. Curr. Microbiol. 4:325–330
    [Google Scholar]
  11. Hampson D. J., Trott D. J. 1995 Intestinal spirochaetal infections of pigs: an overview with an Australian perspective. 139–169 Manipulating pig production V. Australasian Pig Science Association; Werribee, Victoria, Australia:
    [Google Scholar]
  12. Harel J., Forget C. 1995; DNA probe and polymerase chain reaction procedure for the specific detection of Serpulina hyodysenteriae. Mol. Cell. Probes 9:111–119
    [Google Scholar]
  13. Harris D. L., Glock R. D., Christensen C. R., Kinyon J. M. 1972; Swine dysentery: inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction of the disease. Vet. Med. Small Anim. Clin. 67:61–64
    [Google Scholar]
  14. Hovind-Hougen K., Birch-Andersen A., Henrik-Nielsen R., Orholm M., Pedersen J. O., Teglbjærg P. S., Thaysen E. H. 1982; Intestinal spirochetosis: morphological characterization and cultivation of the spirochete Brachyspira aalborgi gen. nov., sp. nov. J. Clin. Microbiol. 16:1127–1136
    [Google Scholar]
  15. Hudson M. J., Alexander T. J. L., Lysons R. J. 1976; Diagnosis of swine dysentery: spirochaetes which may be confused with Treponema hyodysenteriae. Vet. Rec. 99:498–500
    [Google Scholar]
  16. Jensen N. S. National Animal Disease Center; Personal communication
  17. Jensen N. S., Casey T. A., Stanton T. B. 1990; Detection and identification of Treponema hyodysenteriae by using oligodeoxynucleotide probes complementary to’ 16S rRNA. J. Clin. Microbiol. 28:2717–2721
    [Google Scholar]
  18. Jensen N. S., Stanton T. B., Swayne D. E. 1996; Identification of the swine pathogen Serpulina hyodysenteriae in rheas (Rhea americana). Vet, Microbiol. 52:259–269
    [Google Scholar]
  19. Lee J. I., Hampson D. J. 1992; Intestinal spirochaetes colonizing aborigines from communities in the remote north of Western Australia. Epidemiol. Infect. 109:133–141
    [Google Scholar]
  20. Lee J. L, Hampson D. J. 1994; Genetic characterisation of intestinal spirochaetes and their association with disease. J. Med. Microbiol. 40:365–371
    [Google Scholar]
  21. Lee J. I., Hampson D. J., Lymbery A. J., Harders S. J. 1993; The porcine intestinal spirochaetes: identification of new genetic groups. Vet. Microbiol. 34:273–285
    [Google Scholar]
  22. Lemcke R. M., Burrows M. R. 1981; A comparative study of spirochaetes from the porcine alimentary tract. J. Hyg. 86:173–182
    [Google Scholar]
  23. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol. 12:195–206
    [Google Scholar]
  24. McLaren A. J., Trott D. J., Swayne D. E., Oxberry S. L., Hampson D. J. 1997; Genetic and phenotypic characterization of intestinal spirochetes colonizing chickens and allocation of known pathogenic isolates to three distinct genetic groups. J. Clin. Microbiol. 35:412–417
    [Google Scholar]
  25. Mhoma J. R. L., Hampson D. J., Robertson I. D. 1992; A serological survey to establish the prevalence of infection with Treponema hyodysenteriae in Western Australia. Aust. Vet. J. 69:81–84
    [Google Scholar]
  26. Park N. Y., Chung C. Y., McLaren A. J., Atyeo R. F., Hampson D. J. 1995; Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis. FEMS Microbiol. Lett. 125:225–230
    [Google Scholar]
  27. Pettersson B., Fellstrbm C., Andersson A., Uhlen M., Gunnarsson A., Johansson K.-E. 1996; The phylogeny of intestinal spirochetes (Serpulina species) based on sequence analysis of the 16S rRNA gene. J. Bacteriol. 178:4189–4199
    [Google Scholar]
  28. Sagartz J. E., Swayne D. E., Eaton K. A., Hayes J. R., Amass K. D., Wack R., Kramer L. 1992; Necrotizing typhlocolitis associated with a spirochete in rheas (Rhea americana). Avian Dis. 36:282–289
    [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual, 2nd. Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y.:
    [Google Scholar]
  30. Sanna A., Dettori G., Agliand A M., Branca G., Grillo R., Leone F., Rossi A., Parisi G. 1984; Studies of treponemes isolated from human gastrointestinal tract. L’lgiene Moderna 81:959–973
    [Google Scholar]
  31. Smibert R. M. 1984 Genus III. Treponema Schaudinn 1905, I728AL. 4957 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams and Wilkins Co; Baltimore, Md.:
    [Google Scholar]
  32. Stanton T. B. 1992; Proposal to change the genus designation Serpula to Serpulina gen. nov. containing the species Serpulina hyodysenteriae comb, nov. and Serpulina innocens comb. nov. Int. J. Syst. Bacteriol. 42:189–190
    [Google Scholar]
  33. Stanton T. B. 1997 Physiology of ruminal and intestinal spirochaetes. 7 Hampson D. J., Stanton T. B.ed Intestinal spirochaetes in domestic animals and humans CAB International Press; Wallingford, England.:
    [Google Scholar]
  34. Stanton T. B., Jensen N. S., Casey T. A., Tordoff L. A., Dewhirst F. E., Paster B. J. 1991; Reclassification of Treponema hyodysenteriae and Treponema innocens in a new genus, Serpula gen. nov., as Serpula hyodysenteriae comb. nov. and Serpula innocens comb. nov. Int. J. Syst. Bacteriol. 41:50–58
    [Google Scholar]
  35. Stanton T. B., Lebo D. F. 1988; Treponema hyodysenteriae growth under various culture conditions. Vet. Microbiol. 18:177–190
    [Google Scholar]
  36. Stanton T. B., Trott D. J., Lee J. I., McLaren A. J., Hampson D. J., Paster B. J., Jensen N. S. 1996; Differentiation of intestinal spirochetes by multilocus enzyme electrophoresis analysis and 16S rRNA sequence comparisons. FEMS Microbiol. Lett. 136:181–186
    [Google Scholar]
  37. Taylor D. J., Simmons J. R., Laird H. M. 1980; Production of diarrhoea and dysentery in pigs by feeding pure cultures of a spirochaete differing from Treponema hyodysenteriae. Vet. Rec. 106:326–332
    [Google Scholar]
  38. Trott D. J., Huxtable C. R., Hampson D. J. 1996; Experimental infection of newly weaned pigs with human and porcine strains of Serpulina pilosicoli. Infect. Immun. 64:4648–4654
    [Google Scholar]
  39. Trott D. J., Atyeo R. F., Lee J. I., Swayne D. E., Stoutenburg J. W., Hampson D. J. 1996; Genetic relatedness amongst intestinal spirochaetes isolated from rats and birds. Lett. Appl. Microbiol. 23:431–436
    [Google Scholar]
  40. Trott D. J., Jensen N. S., Saint Girons I., Oxberry S. L., Stanton T. B., Lindquist D., Hampson D. J. 1997; Identification and characterization of Serpulina pilosicoli isolates recovered from the blood of critically ill patients. J. Clin. Microbiol. 35:482–485
    [Google Scholar]
  41. Trott D. J., Stanton T. B., Jensen N. S., Duhamel G. E., Johnson J. L., Hampson D. J. 1996; Serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. Int. J. Syst. Bacteriol. 46:206–215
    [Google Scholar]
  42. Trott D. J., Stanton T. B., Jensen N. S., Hampson D. J. 1996; Phenotypic characteristics of Serpulina pilosicoli, the agent of intestinal spirochaetosis. FEMS Microbiol. Lett. 142:209–214
    [Google Scholar]
  43. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol. 37:463–464
    [Google Scholar]
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