1887

Abstract

The recently reported chemotaxonomic and genotypic description of two well-separated subgroups (I and II) and their affiliation to and the unnamed DNA hybridization group (HG) 11 (G. Huys, M. Altwegg, M.-L. HÄnninen, M. Vancanneyt, L. Vauterin, R. Coopman, U. U. Torck, J. Lüthy-Hottenstein, P. Janssen, and K. Kersters, Syst. Appl. Microbiol. 19:616-623, 1996) has questioned the original species descriptions of and . In order to elucidate the uncleartaxonomic status of these taxa in the genus , we have further investigated a collection of 14 reference strains and 14 related isolates encompassing the taxa subgroups I and II, , and HG11 by DNA-DNA hybridization (on 17 of the 28 strains) and phenotypic characterization (on all 28 strains). Genotypically, the investigated strains could be grouped into two DNA hybridization groups that exhibited between-group homologies ranging from 42 to 52%. The members of DNA homology group I (DNA binding, 76 to 100%) were strains of subgroup I, including the type strain LMG 3774, and two -like isolates, leading to the conclusion that these strains should be considered true representatives of the species A eucrenophila. The strains of subgroup II, HG11, and , on the other hand, were closely joined in DNA homology group II (DNA binding, 74 to 105%) together with two presumptive isolates. The fact that strain LMG 16330 was the only type strain residing in DNA homology group I1 implies that HG11 and subgroup II should be classified in the species . Except for the somewhat aberrant phenotypic positions of HG11 strains LMG 13075 and LMG 13076, the establishment of DNA homology groups I and II was supported by the delineation of phena 1 and 2 (level of correlation, 90%), respectively, as revealed by numerical analysis of 136 phenotypic test results. These data indicate and are phenotypically highly related but can be easily separated by testing the production of acid from -cellobiose. and lactose and the assimilation of -cellobiose. Extended descriptions of both species are given.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-4-1157
1997-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/4/ijs-47-4-1157.html?itemId=/content/journal/ijsem/10.1099/00207713-47-4-1157&mimeType=html&fmt=ahah

References

  1. Abott S. L., Cheung W. K. W., Kroske-Bystrom S., Malekzadeh T., Janda M. J. 1992; Identification of Aeromonas strains to the genospecies level in the clinical laboratory. J. Clin. Microbiol. 30:1262–1266
    [Google Scholar]
  2. Ali A., Carnahan A. M., Altwegg M., Liithy-Hottenstein J., Joseph S. W. 1996; Aeromonas bestiarum sp. nov. (formerly genomospecies DNA group 2 A. hydrophila), a new species isolated from non-human sources. Med; Microbiol. Lett. 5:156–165
    [Google Scholar]
  3. Allen D. A., Austin B., Colwell R. R. 1983; Aeromonas media, a new species isolated from river water. Int. J. Syst. Bacteriol. 33:599–604
    [Google Scholar]
  4. Altwegg M. 1993; A polyphasic approach to the classification and identification of Aeromonas strains. Med. Microbiol. Lett. 2:200–205
    [Google Scholar]
  5. Altwegg M., Liithy-Hottenstein J. 1991; Methods for the identification of DNA hybridization groups in the genus Aeromonas. Experientia 47:403–406
    [Google Scholar]
  6. Altwegg M., Steigerwalt A. G., Altwegg-Bissig R., Liithy-Hottenstein J., Brenner D. J. 1990; Biochemical identification of Aeromonas genospecies isolated from humans. J. Clin. Microbiol. 28:258–264
    [Google Scholar]
  7. Carnahan A., Fanning G. R., Joseph S. W. 1991; Aeromonas jandaei (formerly genospecies DNA group 9 A. sobria), a new sucrose-negative species isolated from clinical specimens. J. Clin. Microbiol. 29:560–564
    [Google Scholar]
  8. Carnahan A. M., Altwegg M. 1996 Taxonomy. 1–38 Austin B., Altwegg M., Gosling P. J., Joseph S.ed The genus Aeromonas John Wiley & Sons; Chichester, United Kingdom.:
    [Google Scholar]
  9. Carnahan A. M., Chakraborty T., Fanning G. R., Verma D., Ali A., Janda J. M., Joseph S. W. 1991; Aeromonas trota sp. nov., an ampicillin-susceptible species isolated from clinical specimens. J. Clin. Microbiol. 29:1206–1210
    [Google Scholar]
  10. Carnahan A. M., Joseph S. W. 1993; Systematic assessment of geographically and clinically diverse aeromonads. Syst. Appl. Microbiol. 16:72–84
    [Google Scholar]
  11. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol. 101:738–754
    [Google Scholar]
  12. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  13. Edwards P. R., Fife M. A. 1956; Cyanide media in the differentiation of enteric bacteria. Appl. Microbiol. 4:46
    [Google Scholar]
  14. Esteve C. 1995; Numerical taxonomy of Aeromonadaceae and Vibrionaceae associated with reared fish and surrounding fresh and brackish water. Syst. Appl. Microbiol. 18:391–402
    [Google Scholar]
  15. Esteve C. 1997; Is AFLP fingerprinting a true alternative to the DNA-DNA pairing method to assess genospecies in the genus Aeromonas? Int. J. Syst. Bacteriol. 47:245–246
    [Google Scholar]
  16. Esteve C., Gutierrez M. C., Ventosa A. 1995; Aeromonas encheleia sp. nov., isolated from European eels. Int. J. Syst. Bacteriol. 45:462–466
    [Google Scholar]
  17. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur. J. Biochem. 12:143–153
    [Google Scholar]
  18. Hänninen M.-L., Salmi S., Siitonen A. 1995; Maximum growth temperature ranges of Aeromonas spp. isolated from clinical and environmental sources. Microb. Ecol. 29:259–267
    [Google Scholar]
  19. Hickman-Brenner F. W., Fanning G. R., Arduino M. J., Brenner D. J., Farmer J. J. III 1988; Aeromonas schubertii, a new mannitol-negative species found in human clinical specimens. J. Clin. Microbiol. 26:1561–1564
    [Google Scholar]
  20. Hickman-Brenner F. W., MacDonald K. L., Steigerwalt A. G., Fanning G. R., Brenner D. J., Farmer J. J. III 1987; Aeromonas veronii, a new ornithine decarboxylase-positive species that may cause diarrhea. J. Clin. Microbiol. 25:900–906
    [Google Scholar]
  21. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. J. Bacteriol. 66:24–27
    [Google Scholar]
  22. Huys G., Altwegg M., Hänninen M.-L., Vancanneyt M., Vauterin L., Coopman R., Torek U., Lüthy-Hottenstein J., Janssen F., Kersters K. 1996; Genotypic and chemotaxonomic description of two subgroups in the species Aeromonas eucrenophila and their affiliation to A. encheleia and Aeromonas DNA hybridization group 11. Syst. Appl. Microbiol. 19:616–623
    [Google Scholar]
  23. Janda J. M. 1991; Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with the genus Aeromonas. ClinMicrobiol. Rev. 4:397–410
    [Google Scholar]
  24. Kämpfer P. 1990; Evaluation of the Titertek-Enterobac-Automated system (TTE-AS) for identification of members of the family Enterobacteriaceae. Zentralbl. Bakteriol. 273:341–351
    [Google Scholar]
  25. Kämpfer P., Altwegg M. 1992; Numerical classification and identification of Aeromonas genospecies. J. Appl. Bacteriol. 72:341–351
    [Google Scholar]
  26. Lingappa Y., Lockwood J. L. 1961; A chitin medium for isolation, growth and maintenance of actinomycetales. Nature 189:158–159
    [Google Scholar]
  27. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  28. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  29. Martinez-Murcia A. J., Esteve C., Garay E., Collins M. D. 1992; Aeromonas allosaccharophila sp. nov., a new mesophilic member of the genus Aeromonas. FEMS Microbiol. Lett. 91:199–206
    [Google Scholar]
  30. National Committee for Clinical Laboratory Standards 1993 Approved standard M7-A3. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically National Committee for Clinical Laboratory Standards; Villanova, Pa:
    [Google Scholar]
  31. National Committee for Clinical Laboratory Standards 1993 Tentative standard M6-T. Evaluating production lots of dehydrated Mueller-Hinton agar National Committee for Clinical Laboratory Standards; Villanova, Pa:
    [Google Scholar]
  32. Noterdaeme L., Bigawa S., Steigerwalt A. G., Brenner D. J., Ollevier F. 1996; Numerical taxonomy and biochemical identification of fish associated motile Aeromonas spp. Syst. Appl. Microbiol. 19:624–633
    [Google Scholar]
  33. Popoff M. 1984 Genus Aeromonas Kluyver and van Niel 1936, 398AI. 545–548 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  34. Schubert R. H. W. Personal communication
  35. Schubert R. H. W., Hegazi M. 1988; Aeromonas eucrenophila species nova Aeromonas caviae a later and illegitimate synonym of Aeromonaspunctata. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Grig. Reihe A 268:34–39
    [Google Scholar]
  36. Smibert R. M., Krieg N. R. 1981 General characterization. 409–443 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Briggs Phillips G.ed Manual of methods for general microbiology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  37. Sneath P. H. A., Sokal R. R. 1973 Numerical taxonomy. The principles and practice of numerical classification. Freeman and Co.; San Francisco, Calif:
    [Google Scholar]
  38. Vandamme P., Pot B., Gillis M., De Vos P., Kersters K., Swings J. 1996; Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol. Rev. 60:407–436
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-4-1157
Loading
/content/journal/ijsem/10.1099/00207713-47-4-1157
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error