1887

Abstract

During a study of the prevalence and distribution of gastric helicobacters in domestic pets, a novel group of -like organisms were identified. These “ group 2 strains were initially distinguished from the species and by their cellular morphology and the type of motility exhibited. Bacterial cells were only slightly spiral, 5 to 7 µm long, and 0.8 to 1.2 µm wide and showed an unusual slow wavelike motion. Each cell had tufts of sheathed flagella at one or both ends. Phylogenetic analysis by 16S ribosomal DNA sequence comparison revealed that ” 2, and the new group of helicobacters formed a distinct cluster with intraspecies similarity values of more than 98%. These taxa were clearly separated from all other known species. Dot blot DNA-DNA hybridization studies indicated that the group 2 strains are genetically homogeneous and distinct from other canine and feline gastric helicobacters. Quantitative DNA-DNA hybridization experiments showed that group 2 strains exhibit >;90% DNA homology to each other, but <39% homology to the phylogenetically related taxa and . We propose the name for the novel group 2 strains. The type strain is Inkinen (= CCUG 37845).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-4-975
1997-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/4/ijs-47-4-975.html?itemId=/content/journal/ijsem/10.1099/00207713-47-4-975&mimeType=html&fmt=ahah

References

  1. Andersen L. P., Nørgaard A., Hoick S., Blom J., Elsborg L. 1996; Isolation of a “Helicobacter heilmannii”- like organism from the human stomach. Eur. J. Clin. Microbiol. Infect. Dis. 15:95–96 (Letter.)
    [Google Scholar]
  2. Archer J. R., Romero S., Ritchie A. E., Hamacher M. E., Steiner B. M., Bryner J. H., Schell R. F. 1988; Characterization of an unclassified microaerophilic bacterium associated with gastroenteritis. J. Clin. Microbiol. 26:101–105
    [Google Scholar]
  3. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol. 101:738–754
    [Google Scholar]
  4. Dent J. C., McNulty C. A. M., Uff J. C., Wilkinson S. P., Gear M. W. L. 1987; Spiral organisms in the gastric antrum. Lancet ii:96 (Letter.)
    [Google Scholar]
  5. Eaton K. A., Dewhirst F. E., Paster B. J., Tzellas N., Coleman B. E., Paola J., Sherding R. 1996; Prevalence and varieties of Helicobacter species in dogs from random sources and pet dogs: animal and public health implications. J. Clin. Microbiol. 34:3165–3170
    [Google Scholar]
  6. Fox J. G., Yan L. L., Dewhirst F. E., Paster B. J., Shames B., Murphy J. C., Hayward A., Belcher J. C., Mendes E. N. 1995; Helicobacter bills sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice. J. Clin. Microbiol. 33:445–454
    [Google Scholar]
  7. Franklin C. L., Beckwith C. S., Livingston R. S., Riley L. K., Gibson S. V., Besch-Williford C. L., Hook R. R. Jr. 1996; Isolation of a novel Helicobacter species, Helicobacter cholecystus sp. nov., from the gall bladders of Syrian hamsters with cholangiofibrosis and centrilobular pancreatitis. J. Clin. Microbiol. 34:2952–2958
    [Google Scholar]
  8. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur. J. Biochem. 12:143–153
    [Google Scholar]
  9. Goodwin C. S., Armstrong J. A. 1990; Microbiological aspects of Helicobacter pylori (Campylobacter pylori). Eur. J. Clin. Microbiol. Infect. Dis. 9:1–13
    [Google Scholar]
  10. Hänninen M.-L. 1989; Rapid method for the detection of DNase of Campylobacters. J. Clin. Microbiol. 27:2118–2119
    [Google Scholar]
  11. Hänninen M.-L., Jalava K., Saari S., Happonen I., Westermarck E. 1995; Culture of “Gastrospirillum” from gastric biopsies of dogs. Eur. J. Clin. Microbiol. Infect. Dis. 14:145 (Letter.)
    [Google Scholar]
  12. Hänninen M.-L., Happonen I., Saari S., Jalava K. 1996; Culture and characteristics of Helicobacter bizzozeronii, a new canine gastric Helicobacter sp. Int. J. Syst. Bacteriol. 46:160–166
    [Google Scholar]
  13. Happonen L., Saari S., Castren L., Tyni O., Hanninen M.-L., Westermarck E. 1996; Comparison of diagnostic methods for detecting gastric Helicobacter-like organisms in dogs and cats. J. Comp. Pathol. 115:117–27
    [Google Scholar]
  14. Happonen L., Saari S., Castren L., Tyni O., Hanninen M.-L., Westermarck E. 1996; Occurrence and topographical mapping of gastric Helicobacter-like organisms and their association with histological changes in apparently healthy dogs and cats. J. Vet. Med. Assoc. 43:305–315
    [Google Scholar]
  15. Hartford T., Sneath P. H. A. 1993; Optical DNA-DNA homology in the genus Listeria. Int. J. Syst. Bacteriol. 43:26–1
    [Google Scholar]
  16. Heilmann K. L., Borchard F. 1991; Gastritis due to spiral shaped bacteria other than Helicobacter pylori: clinical, histological and ultrastructural findings. Gut 32:137–40
    [Google Scholar]
  17. Henry G. A., Long P. H., Burns J. L., Charbonneau D. L. 1987; Gastric spirillosis in beagles. Am. J. Vet. Res. 48:831–836
    [Google Scholar]
  18. Huss V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst. Appl. Microbiol. 4:184–192
    [Google Scholar]
  19. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. 21–132 Munro H. N. Mammalian protein metabolism 3 Academic Press, Inc.; New York, N.Y.:
    [Google Scholar]
  20. Kirkbride C. A., Gates C. E., Collins J. E., Ritchie A. E. 1985; Ovine abortion associated with an anaerobic bacterium. J. Am. Vet. Med. Assoc. 186:789–791
    [Google Scholar]
  21. Lee A., Hazell S. L., O’Rourke J., Kouprach S. 1988; Isolation of a spiral-shaped bacterium from the cat stomach. Infect. Immun. 56:2843–2850
    [Google Scholar]
  22. Lockard V. G., Boler R. K. 1970; Ultrastructure of a spiraled microorganism in the gastric mucosa of dogs. Am. J. Vet. Res. 31:1453–1462
    [Google Scholar]
  23. Mendes E. N., Queiroz D. M. M., Dewhirst F. E., Paster B. J., Moura S. B., Fox J. G. 1996; Helicobacter trogontum sp. nov., isolated from the rat intestine. Int. J. Syst. Bacteriol. 46:916–921
    [Google Scholar]
  24. On S. L. W. 1996; Identification methods for Campylobacters, helicobacters and related organisms. Clin. Microbiol. Rev. 9:405–422
    [Google Scholar]
  25. Paster B. J., Dewhirst F. E. 1988; Phylogeny of Campylobacters, wolinellas, Bacteroides gracilis, and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int. J. Syst. Bacteriol. 38:56–62
    [Google Scholar]
  26. Paster B. J., Lee A., Fox J. G., Dewhirst F. E., Tordoff L. A., Fraser G. J., O’Rourke J. L., Taylor N. S., Ferrero R. 1991; Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae, and related bacteria. Int. J. Syst. Bacteriol. 41:31–38
    [Google Scholar]
  27. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol. 8:151–156
    [Google Scholar]
  28. Queiroz D. M. M., Rocha G. A., Mendes E. N., Lage A. P., Carvalho A. C. T., Barbosa A. J. A. 1990; A spiral microorganism in the stomach of pigs. Vet. Microbiol. 24:199–204
    [Google Scholar]
  29. Rappin G. 1881 Contribution à l’étude des bactéries de la bouche à létat normal et dans la fièvre typhoide Collège de France; Nantes: Ph.D. Thesis
    [Google Scholar]
  30. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  31. Salomon H. 1898; Ueber das Spirillum des Sâugetiermagens und sein Verhalten zu den Belegzellen. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Orig. Abt. 1 19:433–42
    [Google Scholar]
  32. Solnick J. V., O’Rourke J., Lee A., Paster B. J., Dewhirst F. E., Tompkins L. S. 1993; An uncultured gastric spiral organism is a newly identified Helicobacter in humans. J. Infect. Dis. 168:379–385
    [Google Scholar]
  33. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44:846–849
    [Google Scholar]
  34. Ursing J. B., Lior H., Owen R. J. 1994; Proposal of minimal standards for describing new species of the family Campylobacteraceae. Int. J. Syst. Bacteriol. 44:842–845
    [Google Scholar]
  35. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. 1991; Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int. J. Syst. Bacteriol. 41:88–103
    [Google Scholar]
  36. Marshall B. J. 1983; Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet i:1273–1275
    [Google Scholar]
  37. Weber A. F., Schmittdiel E. F. 1962; Electron microscopic and bacteriologic studies of spirilla isolated from the fundic stomachs of cats and dogs. Am. J. Vet. Res. 23:422–427
    [Google Scholar]
  38. Wu H., Pelkonen E., Knuutila S., Kaartinen M. 1995; A human follicular lymphoma B cell line hypermutates its functional immunoglobulin genes in vitro. Eur. J. Immunol. 25:3263–3269
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-4-975
Loading
/content/journal/ijsem/10.1099/00207713-47-4-975
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error