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Volume 48, Issue 1

sp. nov. is one of three rhizobial genotypes identified which nodulate and form nitrogen-fixing symbioses with [(L.) Ledebour] Free

Abstract

[(L.) Ledebour] is native to Inner Mongolia where rhizosphere samples were collected for the isolation of 106 rhizobial cultures. Besides nodulating the original trap host, the isolates formed nitrogen-fixing symbioses with Only half of the isolates nodulated alfalfa (). but these did not form nitrogen-fixing symbioses. also formed nitrogen-fixing symbioses with A total of 56 distinctive muitilocus electrophoretic types (ETs) were identified among 94 of the 106 isolates which were analysed for variation in electrophoretic mobility of 12 enzyme loci. One isolate (USDA 1920) possessed a unique ET, while the ETs of the other isolates formed two weakly divergent subgroups approximately equal in size. It was concluded from small subunit rRNA gene sequences of eight isolates of that they belonged to the genus and not to the genus which is more commonly associated with Genomic similarity, determined from DNA hybridization analysis, between USDA 1920 and the strain representing the remaining isolates (USDA 1844) was lower than 20%. Based upon these observations it was concluded that at least three genomic species of rhizobia form nitrogen-fixing symbioses with One of these genomic species is , another is represented by the single isolate USDA 1920 and the name is proposed for the third genomic species represented by USDA 1844.

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Keyword(s): Medicago ruthenica , Rhizobium mongolense sp. nov. and root-nodule bacteria
© Society for General Microbiology, 1998
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1998-01-01
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Rhizobium mongolense sp. nov. is one of three rhizobial genotypes identified which nodulate and form nitrogen-fixing symbioses with Medicago ruthenica [(L.) Ledebour]
Int J Syst Evol Microbiol 48, 13 (1998); https://doi.org/10.1099/00207713-48-1-13
/content/journal/ijsem/10.1099/00207713-48-1-13
/content/journal/ijsem/10.1099/00207713-48-1-13
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